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Tiroglobulin Ölçümlerinin Düsük Titrelerdeki Anti-Tiroglobulinle İnterferansının Arastırılması

Year 2019, Volume: 26 Issue: 3, 296 - 304, 01.09.2019
https://doi.org/10.17343/sdutfd.463906

Abstract

Sonuç:
Bu çalı
şmanın sonuçları TgAb mevcudiyetinin
sük titrelerde bile Tg
ölçümlerini önemli oranda interfere etti
ğini, dilüsyon oranın bu etkiyi değiştirmediğini göstermektedir.
Ayrıca TgAb dı
şındaki
di
ğer otoimmun
antikorların Tg ölçümlerinde interferansa sebep olmadı
ğı ya da çok az olabileceğini söyleyebiliriz.



Bulgu: TgAb
pozitif numunelerdeki recovery sonuçları % 21- % 56 oranında de
ğisirken, TgAb negatif
numunelerininki % 88 - % 96 arasında bulundu.



Gereç-Yöntem:
Bu amaçla dü
sük
ve yüksek TgAb konsantrasyonuna sahip serum havuzlarından alınan örnekler Tg
standartları içerisine ilave edilerek 1/2, 1/10 ve 1/20 dilüsyonla TgAb
konsantrasyonu 5, 10, 20, 40, 60, 80 ve 100 IU/ml olan numuneler hazırlandı.
Aynı sulandırma i
şlemi
TgAb negatif serum havuzları için de yapıldı ve örneklerde Tg ölçümleri
yapılarak geri kazanımlar (recovery) hesaplandı.



Amaç:
Tiroglobulin (Tg), tiroid follikülü için spesifik bir marker olarak kullanılır.
Ancak Tg ölçümlerinde ve dolayısıyla klinik olarak de
ğerlendirilmesindeki en
önemli zorluk tiroglobulin antikorlarının (TgAb) sebep oldu
ğu interferanstır.
Yüksek konsantrasyondaki TgAb’ların serum Tg ölçümünü interfere etti
ği bilinmektedir. Bu
çalı
şmada; düsük konsantrasyonda TgAb
bulunan serumların ve negatif TgAb’lu otoimmun tiroiditli hasta serumlarının Tg
standartlarına ilavesinin Tg ölçümü üzerine interferansının ara
ştırılması amaçlandı.




References

  • 1. Berksöz E. Tiroid Hormonları İnsan Biyokimyası. Onat T, Emerk K, Sönmez EY. editör. 2. baskı. Palme Yayıncılık;2006. p.517-520.
  • 2. Fureraj, T., et al..Role of Thyroglobulin in the Follow Up of the Differentiated Thyroid Cancer in Albania. Cancer 2009;19: 1167-1214.
  • 3. Emerk K. Kanser İnsan Biyokimyası. Onat T, Emerk K, Sönmez EY. editör. 2. baskı. Palme Yayıncılık;2006. p. 717-727.
  • 4. Giovanella, L., et al. Unstimulated highly sensitive thyroglobulin in follow-up of differentiated thyroid cancer patients: a meta-analysis. J Clin Endocrinol Metab 2014; 99(2): 440-447
  • 5. Taylor, P. N., et al. "A review of the clinical consequences of variation in thyroid function within the reference range. J Clin Endocrinol Metab 2013; 98(9): 3562-3571.
  • 6. Ajjan, R. and A. Weetman. Thyroid autoantibody measurement. Thyroid Function Testing, Springer 2010; 109-124.
  • 7. Gao Y, Yuan Z, Yu Y, Lu H Mutual interference between serum thyroglobulin and antithyroglobulin antibody in an automated chemiluminescent immunoassay. Clin Biochem 2007; 735-738-40.
  • 8. Ajjan, R. and A. Weetman Thyroid autoantibody measurement. Thyroid Function Testing, Springer 2010 109-124.
  • 9. Giovanella, L., et al.. Thyroglobulin measurement by highly sensitive assays: focus on laboratory challenges. Clin Chem Lab Med 2015; 53(9): 1301-1314.
  • 10. Gharib, H., et al. American Association of Clinical Endocrinologists, American College of Endocrinology, and Associazione Medici Endocrinologi Medical Guidelines for Clinical practice for the diagnosis and management of thyroid nodules–2016 update. Endocr Pract 2016; 22(s1): 1-60.
  • 11.Okosieme OE, Evans C, Moss L, Parkes AB, Premawardhana LD, Lazarus JH Thyroglobulin antibodies in serum of patients with differentiated thyroid cancer: relationship between epitope specificities and thyroglobulin recovery. Clin Chem 2005; 51:729-34.
  • 12.Rosario PW, Maia FF, Fagundes TA, Vasconcelos FP, Cardoso LD, Purisch S. Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance. Arg Bras Endocrinol Metabol 2004; 48:487-92.
  • 13.Park, J. Y. and L. J. Kricka Interferences in immunoassay. The Immunoassay Handbook (Fourth Edition), Elsevier 2013; 403-416.
  • 14.Spencer, C. A.. Clinical utility of thyroglobulin antibody (TgAb) measurements for patients with differentiated thyroid cancers (DTC). J Clin Endocrinol Metab 2011; 96(12): 3615-3627.
  • 15 Stanojevic, M., et al. Comparison of the influence of thyroglobulin antibodies on serum thyroglobulin values from two different immunoassays in post surgical differentiated thyroid carcinoma patients. J Clin Lab Anal 2009; 23(5): 341-346.
  • 16.Spencer, C. and S. Fatemi. Thyroglobulin antibody (TgAb) methods–Strengths, pitfalls and clinical utility for monitoring TgAb-positive patients with differentiated thyroid cancer. Best Pract Res Clin Endocrinol Metab 2013; 27(5): 701-712.
  • 17.Dufour, D. R.). Thyroglobulin Antibodies and Their Measurement. Thyroid Cancer, Springer 2016; 443-447.
  • 18.Dufour, D. R.. Thyroglobulin antibodies—failing the test, 2011;Oxford University Press.
  • 19.Perros, P., et al. Guidelines for the management of thyroid cancer. Clin Endocrinol 2014; 81(s1): 1-122.
Year 2019, Volume: 26 Issue: 3, 296 - 304, 01.09.2019
https://doi.org/10.17343/sdutfd.463906

Abstract

References

  • 1. Berksöz E. Tiroid Hormonları İnsan Biyokimyası. Onat T, Emerk K, Sönmez EY. editör. 2. baskı. Palme Yayıncılık;2006. p.517-520.
  • 2. Fureraj, T., et al..Role of Thyroglobulin in the Follow Up of the Differentiated Thyroid Cancer in Albania. Cancer 2009;19: 1167-1214.
  • 3. Emerk K. Kanser İnsan Biyokimyası. Onat T, Emerk K, Sönmez EY. editör. 2. baskı. Palme Yayıncılık;2006. p. 717-727.
  • 4. Giovanella, L., et al. Unstimulated highly sensitive thyroglobulin in follow-up of differentiated thyroid cancer patients: a meta-analysis. J Clin Endocrinol Metab 2014; 99(2): 440-447
  • 5. Taylor, P. N., et al. "A review of the clinical consequences of variation in thyroid function within the reference range. J Clin Endocrinol Metab 2013; 98(9): 3562-3571.
  • 6. Ajjan, R. and A. Weetman. Thyroid autoantibody measurement. Thyroid Function Testing, Springer 2010; 109-124.
  • 7. Gao Y, Yuan Z, Yu Y, Lu H Mutual interference between serum thyroglobulin and antithyroglobulin antibody in an automated chemiluminescent immunoassay. Clin Biochem 2007; 735-738-40.
  • 8. Ajjan, R. and A. Weetman Thyroid autoantibody measurement. Thyroid Function Testing, Springer 2010 109-124.
  • 9. Giovanella, L., et al.. Thyroglobulin measurement by highly sensitive assays: focus on laboratory challenges. Clin Chem Lab Med 2015; 53(9): 1301-1314.
  • 10. Gharib, H., et al. American Association of Clinical Endocrinologists, American College of Endocrinology, and Associazione Medici Endocrinologi Medical Guidelines for Clinical practice for the diagnosis and management of thyroid nodules–2016 update. Endocr Pract 2016; 22(s1): 1-60.
  • 11.Okosieme OE, Evans C, Moss L, Parkes AB, Premawardhana LD, Lazarus JH Thyroglobulin antibodies in serum of patients with differentiated thyroid cancer: relationship between epitope specificities and thyroglobulin recovery. Clin Chem 2005; 51:729-34.
  • 12.Rosario PW, Maia FF, Fagundes TA, Vasconcelos FP, Cardoso LD, Purisch S. Antithyroglobulin antibodies in patients with differentiated thyroid carcinoma: methods of detection, interference with serum thyroglobulin measurement and clinical significance. Arg Bras Endocrinol Metabol 2004; 48:487-92.
  • 13.Park, J. Y. and L. J. Kricka Interferences in immunoassay. The Immunoassay Handbook (Fourth Edition), Elsevier 2013; 403-416.
  • 14.Spencer, C. A.. Clinical utility of thyroglobulin antibody (TgAb) measurements for patients with differentiated thyroid cancers (DTC). J Clin Endocrinol Metab 2011; 96(12): 3615-3627.
  • 15 Stanojevic, M., et al. Comparison of the influence of thyroglobulin antibodies on serum thyroglobulin values from two different immunoassays in post surgical differentiated thyroid carcinoma patients. J Clin Lab Anal 2009; 23(5): 341-346.
  • 16.Spencer, C. and S. Fatemi. Thyroglobulin antibody (TgAb) methods–Strengths, pitfalls and clinical utility for monitoring TgAb-positive patients with differentiated thyroid cancer. Best Pract Res Clin Endocrinol Metab 2013; 27(5): 701-712.
  • 17.Dufour, D. R.). Thyroglobulin Antibodies and Their Measurement. Thyroid Cancer, Springer 2016; 443-447.
  • 18.Dufour, D. R.. Thyroglobulin antibodies—failing the test, 2011;Oxford University Press.
  • 19.Perros, P., et al. Guidelines for the management of thyroid cancer. Clin Endocrinol 2014; 81(s1): 1-122.
There are 19 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research Articles
Authors

Erdem Çokluk

Ramazan Şekeroğlu

Bedia Bati This is me

Mustafa Özturk This is me

Publication Date September 1, 2019
Submission Date September 26, 2018
Acceptance Date February 19, 2019
Published in Issue Year 2019 Volume: 26 Issue: 3

Cite

Vancouver Çokluk E, Şekeroğlu R, Bati B, Özturk M. Tiroglobulin Ölçümlerinin Düsük Titrelerdeki Anti-Tiroglobulinle İnterferansının Arastırılması. Med J SDU. 2019;26(3):296-304.

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