PERİODONTİTİS DERECESİ İLE DİŞETİ OLUĞU SIVISI TNF-α VE MIP-1α SEVİYELERİ ARASINDAKİ İLİŞKİNİN DEĞERLENDİRİLMESİ: ÖN ÇALIŞMA

Year 2022, Volume: 29 Issue: 2, 243 - 248, 30.06.2022

Umut Yiğit Fatih Karaaslan Ahu Dikilitaş Esra Özge Aydın

https://doi.org/10.17343/sdutfd.1034024

Abstract

Amaç
Periodontitisin patogenezindeki sitokin ve kemokinlerin
rolü,periodontitisin başlaması ve ilerlemesinde
önemli fonksiyonlara sahip olan ile Tümör Nekroz
Faktör alfa (TNF-α) ve Makrofaj İnflamatuar Protein
1α ile (MIP 1α) gösterilmiştir. Bununla birlikte, farklı
periodontitis derecelerinin sitokin ve kemokin profilleri
hala belirsizdir ve periodontitisin ilerleme hızı ile ilişkili
biyobelirteçler hakkında henüz kesin bilgiler bildirilmemiştir.
Bu nedenle, bu çalışmanın amacı Derece A,
B ve C'deki dişeti oluğu sıvısındaki (DOS) MIP-1α ve
TNF-α'nın düzeylerini tahmin etmek ve periodontitis
derecesini belirlemede güvenilir biyobelirteçler olarak
rollerini değerlendirmektir.
Gereç ve Yöntem
Bireyler periodontitis derecelerine göre Evre IV periodontitis
tanısı alan ve Derece A (Derece A, n = 21),
Evre IV periodontitis tanısı alan ve Derece B (Derece
B, n =21) ve Evre IV periodontitis tanısı alan Derece
C bireyler (Derece C, n = 21) olmak üzere üç gruba
ayrıldı.
Bulgular
Ortalama TNF-α seviyeleri açısından gruplar arasında
anlamlı bir fark olmamasına rağmen, Derece
C’deki ortalama MIP-1α seviyesi Derece B ve Derece
A'dan anlamlı derecede yüksekti. Derece B'deki ortalama
MIP-1α seviyesi Derece A'dan önemli ölçüde
daha yüksek (p < 0.05, Kruskal–Wallis testi) bulundu.
Sonuç: MIP-1α, periodontitis derecesi için tanımlayıcı
bir biyobelirteç olarak klinik kullanıma sahip olabilir.

Keywords

kemokin, sitokin, dişeti oluğu sıvısı ,, periodontitis

Supporting Institution

destekleyen kurum yok

Project Number

proje numarası yok

THE ASSOCIATION BETWEEN GRADE OF PERIODONTITIS AND GCF LEVELS OF TNF-α AND MIP-1α: A PRELIMINARY STUDY

Abstract

Objective
The role of cytokines and chemokines in the
pathogenesis of periodontitis indicates that tumor
necrosis factor alpha (TNF-α) and macrophage
inflammatory protein 1α (MIP1α) have crucial functions
in the initiation and progression of periodontitis.
However, the cytokine and chemokine profiles of
different grades of periodontitis are still unclear, and
no conclusive information has yet been reported
on biomarkers associated with the progression rate
of periodontitis. Thus, the aim of the present study
was to estimate the gingival crevicular fluid (GCF)
levels of MIP-1α and TNF-α in Grades A, B, and C
and to evaluate their role as reliable biomarkers in
determining the grade of periodontitis.
Materials and Methods
Individuals were divided into three groups according
to their grade of periodontitis: individuals diagnosed as
Stage IV periodontitis with Grade A (Grade A, n = 21),
individuals diagnosed as Stage IV periodontitis with
Grade B (Grade B, n = 21), and individuals diagnosed
as Stage IV periodontitis with Grade C (Grade C,
n = 21).
Results
Although there were no significant differences between
groups in terms of mean TNF-α levels, the mean MIP-
1α level of Grade C was significantly higher than that
of Grade B and Grade A. The mean MIP-1α level of
Grade B was significantly higher than that of Grade A
(p < 0.05, Kruskal–Wallis test).
Conclusion
MIP-1α could have clinical utility as a screening
biomarker for the grade of periodontitis.

Keywords

Chemokines, cytokines, gingival crevicular fluid, periodontitis

Project Number

proje numarası yok

References

• 1. Slots J. Periodontitis: facts, fallacies and the future. Periodontology 2000. 2017;75(1):7-23.
• 2. Stadler A, Angst P, Arce R, Gomes S, Oppermann R, Susin C. Gingival crevicular fluid levels of cytokines/chemokines in chronic periodontitis: a meta-analysis. Journal of Clinical Periodontology. 2016;43(9):727-745.
• 3. Afacan B, Öztürk VÖ, Paşalı Ç, Bozkurt E, Köse T, Emingil G. Gingival crevicular fluid and salivary HIF-1α, VEGF, and TNF-α levels in periodontal health and disease. Journal of Periodontology 2019;90(7):788-797.
• 4. Afacan B, Keleş Yücel Z, Paşali Ç, Atmaca İlhan H, Köse T, Emingil G. Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients. Journal of Periodontology. 2020;91(11):1495-1502.
• 5. Nisha K, Suresh A, Anilkumar A, Padmanabhan S. MIP-1α and MCP-1 as salivary biomarkers in periodontal disease. The Saudi Dental Journal. 2018;30(4):292-298.
• 6. Miranda T, Figueiredo N, Figueiredo L, Silva H, Rocha F, Duarte P. Cytokine profiles of healthy and diseased sites in individuals with periodontitis. Archives of Oral Biology. 2020;120:104957.
• 7. Tonetti M, Greenwell H, Kornman K. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. Journal of Periodontology. 2018;89:S159-S172.
• 8. Caton J, Armitage G, Berglundh T, Chapple I, Jepsen S, Kornman K et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. Journal of Clinical Periodontology. 2018;45:S1-S8.
• 9. Graetz C, Mann L, Krois J, Sälzer S, Kahl M, Springer C et al. Comparison of periodontitis patients’ classification in the 2018 versus 1999 classification. Journal of Clinical Periodontology. 2019;46(9):908-917.
• 10. Karaaslan F, Dikilitaş A. The association between stage‐grade of periodontitis and sleep quality and oral health‒related quality of life. Journal of Periodontology. 2019;90(10):1133-1141.
• 11. Silness J, Löe H. Periodontal Disease in Pregnancy II. Correlation Between Oral Hygiene and Periodontal Condition. Acta Odontologica Scandinavica. 1964;22(1):121-135.
• 12. Löe H. The Gingival Index, the Plaque Index and the Retention Index Systems. Journal of Periodontology. 1967;38(6):610-616.
• 13. Dietrich T, Ower P, Tank M, West N, Walter C, Needleman I et al. Periodontal diagnosis in the context of the 2017 classification system of periodontal diseases and conditions – implementation in clinical practice. British Dental Journal. 2019;226(1):16-22.
• 14. Papapanou P, Sanz M, Buduneli N, Dietrich T, Feres M, Fine D et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. Journal of Periodontology. 2018;89:S173-S182.
• 15. Shiau H, Reynolds M. Sex Differences in Destructive Periodontal Disease: A Systematic Review. Journal of Periodontology. 2010;81(10):1379-1389.
• 16. Shiau H. Periodontal Disease in Women and Men. Current Oral Health Reports. 2018;5(4):250-254.
• 17. Fine D, Markowitz K, Fairlie K, Tischio-Bereski D, Ferrandiz J, Godboley D et al. Macrophage Inflammatory Protein-1α Shows Predictive Value as a Risk Marker for Subjects and Sites Vulnerable to Bone Loss in a Longitudinal Model of Aggressive Periodontitis. PLoS ONE. 2014;9(6):e98541.
• 18. Ryu O, Choi S, Linares A, Song I, Kim Y, Jang K et al. Gingival Epithelial Cell Expression of Macrophage Inflammatory Protein-1α Induced by Interleukin-1β and Lipopolysaccharide. Journal of Periodontology. 2007;78(8):1627-1634.
• 19. Emingil G, Atilla G, Baskesen A, Berdeli A. Gingival crevicular fluid EMAP-II, MIP-1alpha and MIP-1beta levels of patients with periodontal disease. Journal of Clinical Periodontology. 2005;32(8):880-885.
• 20. Politou M, Terpos E, Anagnostopoulos A, Szydlo R, Laffan M, Layton M et al. Role of receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin and macrophage protein 1-alpha (MIP-1a) in monoclonal gammopathy of undetermined significance (MGUS). British Journal of Haematology. 2004;126(5):686-689.