isubu jaf Ziraat Fakültesi Dergisi 1304-9984 2687-3419 Isparta University of Applied Sciences Agricultural Engineering Ziraat Mühendisliği Arpada Hücresel Apoptoz Hassasiyet (CAS) Geninin Susturularak Külleme Hastalığına Karşı Dirençte Etkisinin Belirlenmesi Ersoy Figen 10 12 2018 13 1 40 51 02 02 2017 06 06 2018 Copyright © 2006, Journal of Agriculture Faculty 2006 Journal of Agriculture Faculty

Ekonomik olarak önemlibir tahıl bitkisi olan arpa, dünyada ekim alanı açısından buğday, çeltik vemısırdan sonra gelmektedir. Blumeria graminis f. sp. hordei (Bgh)tarafından oluşturulan külleme hastalığı arpanın en önemli hastalıklarındanbiridir. Hücresel Apoptoz Hassasiyet (Cellular apoptosis susceptiblity; CAS) geninin, arpada külleme hastalığısırasında ekspresyonunun arttığı tespit edilmiştir. R geni tarafındanyönetilen sinyal iletim mekanizmaları hakkında kısıtlı bilgi bulunduğu için,CAS proteininin külleme direnci veya hassasiyeti sırasındaki fonksiyonuanlaşılmaya çalışılmıştır. CAS proteininin patojene karşı oluşan tepkisırasında rolünü anlamak için Arpa Çizgili Mozaik Virüsü (BSMV) tarafından indüklenengen susturma metodu uygulanmıştır. HvCAS geni susturulan bitkiörneklerinde kontrol grubuna (BSMV:00) göre mRNA seviyesi % 54 azalmıştır.Fakat HvCAS geni susturulan bitki örneklerinde külleme hastalığına karşıdirenç veya hassasiyet tepkisinde önemli bir değişim saptanmamıştır.

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