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Farklı Periodontal Hastalık Tiplerinde Tükürük ve Dişeti Oluğu Sıvısı Developmental Endothelial Locus-1 (Del-1) ve Lymphocytw function-associated antigen-1 (LFA-1) Seviyelerinin Değerlendirilmesi

Year 2021, Volume: 8 Issue: 1, 61 - 68, 30.04.2021
https://doi.org/10.15311/selcukdentj.635681

Abstract

Amaç: Bu
çalışmanın amacı; farklı yıkım paterni gösteren periodontal hastalığa sahip
bireylerde nötrofil adezyon yolağının sıkı adezyon basamağında düzenleyici
sitokinler olan
Developmental Endothelial
Locus
-1(Del-1) ve Lymphocyte
function-associated antigen
-1(LFA-1) tükürük ve dişeti oluğu sıvısı
seviyelerinin değerlendirilmesidir.

Gereç ve Yöntemler:Selçuk
Üniversitesi, Diş Hekimliği Fakültesi Periodontoloji Anabilim Dalı’na başvuran
sağlıklı ve sigara içmeyen 42 birey çalışmaya dahil edildi. Klinik ve
radyolojik muayene sonucu
Grade B periodontitis(n=23) ve Grade C
periodontitis (n=19) grubuna dahil edilen bireylerin sondlama cep
derinliği(SCD), klinik ataçman seviyesi(KAS), plak indeks(Pİ), gingival
indeks(Gİ), sondlamada kanama yüzdesi(SKY) gibi klinik kayıtlarının alınmasını
takiben tükürük ve dişeti oluğu sıvısı(DOS) örnekleri toplandı. Del-1 ve LFA-1
sitokin seviyeleri ELISA yöntemi ile değerlendirildi. İstatistiksel analiz için
gruplar arası karşılaştırmalarda Mann-Whitney U testi kullanıldı.p<0.05
istatistiksel anlamlılık düzeyi olarak belirlendi.

Bulgular::Klinik parametreler değerlendirildiğinde; SCD, Pİ ve SKY değerleri
anlamlı şekilde, KAS ve Gİ değerlerinin anlamlı olmaksızın
Grade C periodontitis
grubunda yüksek olduğu görüldü.ELISA analizleri sonucu LFA-1 ve Del-1 tükürük
ve DOS seviyelerinde gruplar arasında anlamlı fark izlenmedi.Proenflamatuvar LFA-1
tükürük ve DOS seviyelerinin, görece daha şiddetli periodontal hastalığa sahip
bireylerin bulunduğu
Grade C grubunda daha yüksek olma eğiliminde olduğu
görüldü.LFA-1 antogonisti olarak bilinen Del-1 tükürük seviyelerinin
Grade
B grubunda, DOS Del-1 seviyelerinin ise
Grade C grubunda yüksek olma
eğilimi olduğu tespit edildi.

Sonuçlar:Bu çalışma ile, nötrofillerin transendotelyal
migrasyonunda rol oynayan Del-1 ve LFA-1 sitokin düzeylerinin periodontal
hastalık şiddeti ile ilgili bilgi verebileceği ancak bu sitokinlerin
periodontal hastalıktaki rollerinin kesin olarak belirlenebilmeleri için ileri
çalışmalara ihtiyaç olduğu görülmektedir.

Anahtar Kelimeler:Dişeti
Oluğu Sıvısı,Periodontitis, Sitokin, Tükürük

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştıma Projeleri Koordinatörlüğü

Project Number

17102013

Thanks

Sitokinlerin analizinde ELISA deneylerinin gerçekleştirilmesindeki yardımları için Niyazi Dündar’a, proje desteği için Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğüne teşekkür ederiz. Bu çalışma Emine Elif Mutafcılar’ın uzmanlık tezinden türetilmiştir.

References

  • Referans1. Lang NP, Lindhe J. Clinical periodontology and implant dentistry, 2 Volume Set: John Wiley & Sons; 2015.
  • Referans2. Hajishengallis G, Chavakis TJTii. Endogenous modulators of inflammatory cell recruitment. 2013;34(1):1-6.
  • Referans3. Shin J, Hosur KB, Pyaram K, Jotwani R, Liang S, Chavakis T, et al. Expression and function of the homeostatic molecule Del-1 in endothelial cells and the periodontal tissue. Clinical and developmental immunology. 2013;2013.
  • Referans4. Rezaee M, Penta K, Quertermous T. Del1 mediates VSMC adhesion, migration, and proliferation through interaction with integrin αvβ3. American Journal of Physiology-Heart and Circulatory Physiology. 2002;282(5):H1924-H32.
  • Referans5. Kourtzelis I, Li X, Mitroulis I, Grosser D, Kajikawa T, Wang B, et al. DEL-1 promotes macrophage efferocytosis and clearance of inflammation. 2019;20(1):40.
  • Referans6. Verma NK, Kelleher DJTJoI. Not just an adhesion molecule: LFA-1 contact tunes the T lymphocyte program. 2017;199(4):1213-21.
  • Referans7. Armitage GCJAop. Development of a classification system for periodontal diseases and conditions. 1999;4(1):1-6.
  • Referans8. Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. Journal of periodontology. 2018;89:S159-S72.
  • Referans9. Silness J, Löe HJAos. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. 1964;22(1):121-35.
  • Referans10. Löe H, Silness JJAos. Periodontal disease in pregnancy I. Prevalence and severity. 1963;21(6):533-51.
  • Referans11. Löe HJTJoP. The gingival index, the plaque index and the retention index systems. 1967;38(6P2):610-6.
  • Referans12. Choi EY, Chavakis E, Czabanka MA, Langer HF, Fraemohs L, Economopoulou M, et al. Del-1, an endogenous leukocyte-endothelial adhesion inhibitor, limits inflammatory cell recruitment. 2008;322(5904):1101-4.
  • Referans13. Eskan MA, Jotwani R, Abe T, Chmelar J, Lim J-H, Liang S, et al. The leukocyte integrin antagonist Del-1 inhibits IL-17-mediated inflammatory bone loss. 2012;13(5):465.
  • Referans14. İnönü E, Seyit Ali Kayıs, Eskan MA, HAKKI SS. Salivary Del-1, IL-17, and LFA-1 Levels in Periodontal Health and Disease. Journal of Periodontal Research. 2019.
  • Referans15. Verma NK, Dempsey E, Long A, Davies A, Barry SP, Fallon PG, et al. Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-β signaling. 2012;287(32):27204-16.
  • Referans16. Smith A, Stanley P, Jones K, Svensson L, McDowall A, Hogg NJIr. The role of the integrin LFA‐1 in T‐lymphocyte migration. 2007;218(1):135-46.
  • Referans17. Ramgolam VS, DeGregorio SD, Rao GK, Collinge M, Subaran SS, Markovic-Plese S, et al. T cell LFA-1 engagement induces HuR-dependent cytokine mRNA stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 signaling cascade. 2010;5(12):e14450.

Salivary and Gingival Crecivular Fluid levels of Developmental Endothelial Locus-1 (Del-1) and Lymphocyte function-associated antigen-1(LFA-1) in Different Types of Periodontal Diseases

Year 2021, Volume: 8 Issue: 1, 61 - 68, 30.04.2021
https://doi.org/10.15311/selcukdentj.635681

Abstract

Background:The
aim of this study is to evaluate salivary and gingival crevicular fluid(GCF)
levels of Developmental Endothelial Locus-1(Del-1) and Lymphocyte
function-associated antigen-1(LFA-1) which are regulators of firm adhesion of
neutrophils in neutrophil recruitment in different grades of periodontitis.

Methods:A
total of 42 non-smoker and healthy individuals who applied to Selcuk University,
Faculty of Dentistry, Department of Periodontology were included in this study.
After clinical and radiographical examination, patients were grouped as Grade
B(n=23) or Grade C(n=19).Probing pocket depth(PPD), clinical attachment
level(CAL), plaque(Pİ) and, gingival index(Gİ) and bleeding sites on probing(BOP)
were recorded.Saliva and GCF samples were collected and cytokine analysis were
performed by ELISA. Mann-Whitney U test is used for statistical analysis and level
of significance
was
determined as
p ≤0.05.

Results:All clinical parameters were found higher in Grade C
periodontitis group, while CAL and GI values showed no significance.In
biochemical analysis, there was no significance between groups in salivary and GCF
levels of LFA-1 and Del-1.Besides these findings;there was a tendency to be
higher in salivary and GCF levels of LFA-1 in Grade C group.As an antagonist
mediator of LFA-1, salivary Del-1 tends to be higher in Grade B periodontitis
group while GCF levels of
  Del-1 tends to
be higher in Grade C periodontitis group.

Conclusions:Findings of this study suggested that salivary
and GCF level of Del-1 and LFA-1 might give information about disease severity.Further
longitudinal studies with greater numbers of participants are required to define
the roles of these cytokines in periodontal pathogenesis.

Keywords: cytokine, gingival crevicular fluid, periodontitis, salivary

Project Number

17102013

References

  • Referans1. Lang NP, Lindhe J. Clinical periodontology and implant dentistry, 2 Volume Set: John Wiley & Sons; 2015.
  • Referans2. Hajishengallis G, Chavakis TJTii. Endogenous modulators of inflammatory cell recruitment. 2013;34(1):1-6.
  • Referans3. Shin J, Hosur KB, Pyaram K, Jotwani R, Liang S, Chavakis T, et al. Expression and function of the homeostatic molecule Del-1 in endothelial cells and the periodontal tissue. Clinical and developmental immunology. 2013;2013.
  • Referans4. Rezaee M, Penta K, Quertermous T. Del1 mediates VSMC adhesion, migration, and proliferation through interaction with integrin αvβ3. American Journal of Physiology-Heart and Circulatory Physiology. 2002;282(5):H1924-H32.
  • Referans5. Kourtzelis I, Li X, Mitroulis I, Grosser D, Kajikawa T, Wang B, et al. DEL-1 promotes macrophage efferocytosis and clearance of inflammation. 2019;20(1):40.
  • Referans6. Verma NK, Kelleher DJTJoI. Not just an adhesion molecule: LFA-1 contact tunes the T lymphocyte program. 2017;199(4):1213-21.
  • Referans7. Armitage GCJAop. Development of a classification system for periodontal diseases and conditions. 1999;4(1):1-6.
  • Referans8. Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. Journal of periodontology. 2018;89:S159-S72.
  • Referans9. Silness J, Löe HJAos. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. 1964;22(1):121-35.
  • Referans10. Löe H, Silness JJAos. Periodontal disease in pregnancy I. Prevalence and severity. 1963;21(6):533-51.
  • Referans11. Löe HJTJoP. The gingival index, the plaque index and the retention index systems. 1967;38(6P2):610-6.
  • Referans12. Choi EY, Chavakis E, Czabanka MA, Langer HF, Fraemohs L, Economopoulou M, et al. Del-1, an endogenous leukocyte-endothelial adhesion inhibitor, limits inflammatory cell recruitment. 2008;322(5904):1101-4.
  • Referans13. Eskan MA, Jotwani R, Abe T, Chmelar J, Lim J-H, Liang S, et al. The leukocyte integrin antagonist Del-1 inhibits IL-17-mediated inflammatory bone loss. 2012;13(5):465.
  • Referans14. İnönü E, Seyit Ali Kayıs, Eskan MA, HAKKI SS. Salivary Del-1, IL-17, and LFA-1 Levels in Periodontal Health and Disease. Journal of Periodontal Research. 2019.
  • Referans15. Verma NK, Dempsey E, Long A, Davies A, Barry SP, Fallon PG, et al. Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-β signaling. 2012;287(32):27204-16.
  • Referans16. Smith A, Stanley P, Jones K, Svensson L, McDowall A, Hogg NJIr. The role of the integrin LFA‐1 in T‐lymphocyte migration. 2007;218(1):135-46.
  • Referans17. Ramgolam VS, DeGregorio SD, Rao GK, Collinge M, Subaran SS, Markovic-Plese S, et al. T cell LFA-1 engagement induces HuR-dependent cytokine mRNA stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 signaling cascade. 2010;5(12):e14450.
There are 17 citations in total.

Details

Primary Language Turkish
Subjects Dentistry
Journal Section Research
Authors

Emine Mutafcilar 0000-0003-1882-553X

Seyit Kayıs

Sema Hakkı 0000-0001-8665-6235

Project Number 17102013
Publication Date April 30, 2021
Submission Date October 22, 2019
Published in Issue Year 2021 Volume: 8 Issue: 1

Cite

Vancouver Mutafcilar E, Kayıs S, Hakkı S. Farklı Periodontal Hastalık Tiplerinde Tükürük ve Dişeti Oluğu Sıvısı Developmental Endothelial Locus-1 (Del-1) ve Lymphocytw function-associated antigen-1 (LFA-1) Seviyelerinin Değerlendirilmesi. Selcuk Dent J. 2021;8(1):61-8.