Determination of the Effects of Heavy Metal Stress on Plant Development and Physiology in Feed Soybean (Glycine Max L.)

Year 2023, Volume: 37 Issue: 3, 430 - 443, 29.12.2023

Gönül Sağlam Koçak Zeynep Gül

Abstract

This study was carried out to determine the tolerance level of cadmium and lead applications at different concentrations on physiological and morphological parameters in soybean (Glycine max L.) The experiment was carried out in Atatürk University Plant Production Application and Research Center greenhouses. Yeşilsoy variety of feed soybean (Glycine max L.) was used as plant material. Plant growth (seedling height, stem diameter, fresh and dry weight, etc.), physiological properties (tissue proportional water content, tissue electrical conductivity) and biochemical parameters (chlorophyll amount, superoxide distumase (SOD), catalase (CAT), heavy metal applications) peroxidase (POD) enzyme activities, etc.) were investigated. All treatments had negative effects on all parameters compared to the control group. Growth retardation was experienced at the highest concentrations applied, but plant deaths were not observed. Although it is known that cadmium metal is more toxic than lead metal, the wet weight values of the plants to which the highest dose of both metals were applied were included in the same statistical group.

Keywords

Soybean, Heavy Metal Stress, Cadmium, Lead, Phytoremediation

References

• Agarwal S, Pandey V (2004). Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Pantarum 48(4): 555-560.
• Ahmad P, Nabi G, Ashraf M (2011). Cadmium-induced oxidative damage in mustard [(Brassica juncea L.) Czern.& Coss.] plants can be alleviated by salicylic acid. South African Journal of Botany 77(1): 36-44.
• Aksoy A (1995). Kayseri-Kırşehir karayolu kenarında yetişen bitkilerde ağır metal kirlenmesi. II. Ulusal Ekoloji Ve Çevre Kongresi 1-8.
• Aksu G (2019). Kadmiyum ile kirli alanlarda bitki besin elementlerinin alınımı üzerine indol asetik asitin etkisi. Toprak Bilimi ve Bitki Besleme Dergisi 7 (2): 80- 85.
• Alım Z (2020). Hümik Asit Uygulamalarının Ağır Metal Stresi Altında Yetiştirilen Terede Bitki Gelişimi ile Bazı Fizyolojik ve Biyokimyasal Özllikler Üzerine Etkileri. Yüksek Lisans Tezi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Erzurum.
• Alyemeni MN, Ahanger MA, Wijaya L, Alam P, Ahmad P (2017). Contrasting tolerance among soybean genotypes subjected to different levels of cadmium stress. Pak. J. Bot. 49(3): 903-911.
• Angelini R, Manes F, Federico R (1990). Spatial and functional correlation between diamine-oxidase and peroxidase activities and their dependence upon de-etiolation and wounding in chick-pea stems. Planta 182(1): 89-96.
• Anonim (2021). Web Adresi:https://arastirma.tarimorman.gov.tr/tepge/Belgeler/PDF%20Tar% C4%B1m%20%C3%9Cr%C3%BCnleri%20Piyasalar%C4%B1/2021-Ocak%20Tar% C4%B1m%20%C3%9Cr%C3%BCnleri%20Raporu/Soya,%20Ocak-2021,%20Tar% C4%B1m%20%C3%9Cr%C3%BCnleri%20Piyasa%20Raporu--.pdf, Erişim Tarihi: 16.08.2022.
• Arıoğlu HH (2007). Yağ bitkileri yetiştirme ve ıslahı ders kitabı. Çukurova Üniversitesi Ziraat Fakültesi ofset atölyesi, Adana.
• Aslam MU, Raza MAS, Saleem MF, Wagas M, Iqbal R, Ahmad S, Haider I (2020). Improving strategic growth stage-based drought tolerance in quinoa by rhizobacterial inoculation. Community Soil Science Plant Anal. 51 (5): 1-16.
• Asri FÖ, Sönmez S (2007). Kadmiyumun çevre ve insan sağlığı üzerine etkileri. Derim, 24(1): 32-39.
• Bachir LD, Wu F, Zhang G, Wu H (2004). Genotypic difference in effect of cadmium on development and mineral concentrations of cotton. Communications in Soil Science and Plant Analysis 35(1-2): 285-299.
• Baker AJ, Brook R (1989). Terrestrial higher plants which hyperaccumulate metallic elements. A review of their distribution, ecology and phytochemistry. Biorecover, 1(2): 81-126.
• Balcı T (2019). Ağır metal stresinin roka (Eruca sativa L.)’da bitki gelişimi ve fizyolojisi üzerine etkisi. Yüksek Lisans Tezi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Erzurum.
• Barcelo J, Poschenrieder C, Andreu I, Gunse B (1986). Cadmium-induced decrease of water stress resistance in bush bean plants (Phaseolus vulgaris L. cv. Contender). I. Effects of Cd on water potential, relative water content and cell wall elasticity. J. Plant Physiol, 125: 17-25.
• Bayçu G (1997). Picea abies’te Kadmiyum Toksisitesi ve Köklerde Kadmiyum Birikimi. XIII. Ulusal Biyoloji Kongresi, 17(20): 433-442.
• Bera AK, Bera A, Samadrita BR (2005). Impact of heavy metal pollution in plants. Developments in physiology biochemstry and molecular biology of plants 1: 105-124.
• Bhaduri AM, Fulekar MH (2012). Antioxidant enzyme responses of plants to heavy metal stress. Reviews in Environmental Science and Bio-Technology 11: 55–69.
• Cihangir N, Sağlam N (1999). Removal of cadmium by Pleurotus sajor-caju basidiomycetes. Acta Biotechnologica, 19(2): 171-177.
• Clemens S, Palmgren MG, Kramer U (2002). A long way ahead: understanding and engineering plant metal accumulation. Trends in Plant Science, 7(7): 309-315.
• Costa G, Morel JL (1994). Water relations, gas exchange and amino acid content in Cd-treated lettuce. Plant Physiol, Biochem 32: 561-570.
• Çağlarırmak N, Hepçimen AZ (2010). Ağır metal toprak kirliliğinin gıda zinciri ve insan sağlığına etkisi. Akademik Gıda 8(2): 31-35.
• Çatak E, Çolak G, Tokur S, Bilgiç O (2000). Bazı domates ve tütün genotiplerinde kadmiyum etkilerini inceleyen istatiksel bir çalışma. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2(1): 13-41.
• De Jhonge WR, Adams FC (1986). Biogeochemical cycling of organic lead compounds. Toxic metals in the atmosphere 561-594.
• Doğru A (2020). Bitkilerde kurşın toksisitesi ve kurşun toleransı. Black Sea Journal of Agriculture 3(4): 329-339.
• Dushenkov S, Kapulnik Y, Blaylock M, Sorochisky B, Raskin I, Ensley B (1997). Phytoremediation: a novel approach to an old problem. In Studiesin Environmental Science 66: 563-572.
• Ehlert C, Maurel C, Tardieu F, Simonneau T (2009). Aquaporin mediated reduction in maize root hydraulic conductivity impacts cell turgor and leaf elongation even without changing transpiration. Plant Physiology 150(2): 1093-1104.
• Fahr M, Laplaze L, Bendaou N, Hocher V, Mzibri ME, Bogusz D (2013). Effect of lead on root growt. Frontiers in Plant Science 4: 175.
• FAO (2019). Crop description and climate. FAO Stat. Food and agriculture organization of the United Nations.
• Finger-Teixeira A, Ferrarese MDLL, Soares AR, Da Silva D, Ferrarese-Filho O (2010). Cadmium-induced lignification restricts soybean root growth. Ecotoxicology and Environmental Safety 73(8): 1959-1964.
• Gardea-Torresdey JL, Peralta-Videa JR., De La Rosa G, Parsons JG (2005). Phytoremediation of heavy metals and study of the metal coordination by X-ray absorption spectrosopy. Coordination Chemistry Reviews 249(17-18): 1797-1810.
• Glass DJ, Raskin I, Ensley BD (1999). Economic patential of phytoremediation. Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment. New York, USA: John Wiley, Sans.
• Groppa MD, Rosales EP, Lannone MF, Benavides MP (2008). Nitric oxide, polyamines and Cd-induced phytotoxicity in wheat roots. Phytochemistry 69(14): 2609-2615.
• Gupta D, Nicoloso F, Schetinger M, Rossato L, Pereira L, Castro G, Srivastava S, Tripathi R (2009). Antioxidant defense mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. J Hazard Mater 172(1): 479-484.
• Gullap MK, Severoglu S, Karabacak T, Yazici A, Ekinci M, Turan M, Yildirim E (2022). Biochar derived from hazelnut shells mitigates the impact of drought stress on soybean seedlings. New Zealand Journal of Crop and Horticultural Science 1-19.
• Hanna WJ, Grant CL (1962). Spectrochemical analysis of the foliage of certain trees and ornamentals for 23 elements. Bulletin of the Torrey Botanical Club 293-302.
• Havir EA, McHale NA (1987). Biochemical and developmental characterization of multiple forms of catalese in tobacco leaves. Plant physiology 84(2): 450-455.
• Huang CY, Bazzaz FA, Vanderhoef LN (1974). The inhibition of soybean metabolism by cadmium and lead. Plant physiology 54(1): 122-124.
• Jayakumar K, Jaleel CA, Vijayarengan P (2007). Changes in growth, biochemical constituents, and antioxidant potentials in radish (Raphanus sativus L.) under cobalt stress. Turkish Journal of Biology 31(3):127–136.
• John R, Ahmad P, Gadgil K, Sharma S (2009). Heavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. International journal of plant production 3(3): 66-75.
• Kabata-Pendias A, Pendias H (1999). Biogeochemistry of trace elements. Pwn, Warszava, 400.
• Kahvecioğlu Ö, Kartal G, Güven A, Timur S (2007). Metallerin çevresel etkileri-I. Metalurji dergisi 136: 47-53.
• Kahvecioğlu Ö, Kartal G, Güven A, Timur S (2010). Metallerin çevresel etkileri- 1. İTÜ Metalürji ve Malzeme Mühendisliği Bölümü.
• Kalinowska A (1984). Lead concentrations in the slug Arion rufus from sites at different distances from a tourist road. Ecolocigal bulletins, 46-49.
• Karanlık S Ergün N, Tiryakioğlu M (2013). Farklı kadmiyum düzeylerinin pamuk bitkisinde (Gossipium hirsutum L.) büyüme, Cd, Fe, Zn konsantrasyonu ve antioksidatif enzim aktiviteleri üzerine etkisi. Tarım Bilimleri Araştırma Dergisi 6 (2): 83-88.
• Kaya C, Higgs D, Ince F, Amador BM, Cakir A, Sakar E (2003). Ameliorative effects of potassium phosphate on salt-stressed pepper and cucumber. Journal of Plant Nutrition 26: 807-820.
• Langille WM, Maclean KS (1976). Some essential nutrient elements in forest plants as related to species, plant part, season and location. Plant And Soil 45(1): 17-26.
• Lichtenthaler H, Wellburm AR (1983). Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents. Biochemical Society Transactions 11(5):591–593.
• Long SP, Ainsworth EA, Davey PA, Bernacchi CJ, Dermody OC (2002). A meta-analysis of elevated [CO2] effects on soybean (Glycine max.) physiology, growth and yield. Global Change Biology 8(8): 695-709.
• Luck H (1965). Catalase. In Bergmeyer, H.U., Ed., Method of Enzymatic Analysis, Academic Press, New York and London 885-894.
• Lyu J, Park J, Pandey KL, Choid S, Leed H, Saeger JD, Depuydt S, Han T (2018). Testing the toxicity of metals, phenol, effluents, and receiving waters by root elongation in Lactuca sativa L., Ecotoxicology and Environmental Safety 149: 225-232.
• Manousaki E, Kalogerakis N (2009). Phytoextraction of Pb and Cd by the Mediterranean saltbush (Atriplex halimus L.). Metal Uptake in Relation to Salinity, Environ. Sci. Pollut. R. 16:844-854.
• Marschner H (2008). Mineral nutrition of higher plants. Academic press, Second edition. London.
• Melo LCA, Alleoni LRF, Carvalho G, Azevedo RA (2011). Cadmium and barium toxicity effects on growth and antioxidant capacity of soybean (Glycine max L.) plants, grown in two soil types with different physicochemical properties. Journal of Plant Nurition and Soil Science 174(5): 847-859.
• Mengel KK (2001). Principles of plant nutrition.Dortrecht: Kluwer academic publishers.
• Mengoni A, Gonnelli C, Galardi F, Gabbrielli R, Bazzicalupo M (2000). Genetic diversity and heavy metal tolerance in populations of Silene paradoxa L. (Caryophyllaceae): a random amplified polymorphic DNA analysis. Molecular Ecology 9:1319-1324.
• Metin M, İlker E (2016). Ana ürün koşullarında bazı soya (Glycine max. L. Merill) hat ve çeşitlerinin Aksaray bölgesine adaptasyonu üzerine çalışmalar. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 25(2): 176-181.
• Mikhailenko AV, Ruban DA, Ermolaev VA, Van Loon AJ (2020). Cadmium pollution in the tourism environment: a literature review. Geoscienses 6(10): 242.
• Munzuroğlu Ö, Gür N (2000). Ağır Metallerin Elma (Malus sylvestris Miller cv. Golden)’da Polen Çimlenmesi ve Polen Tüpü Gelişimi Üzerine Etkileri. Türk Biyoloji Dergisi 24: 677-684. Nazlıcan AN (2010). Soya yetiştiriciliği. www.cukurovataem.gov.tr/upload/ 2010/.../soyayetistiriciligi_ l.pdf. (Erişim Tarihi:13.10.2017).
• Niess DH (1999). Microbial heavy-metal resistance. Applied Microbiology And Biotechnology 51(6):730-750.
• Okçu M, Tozlu E, Kumlay M, Pehluvan M (2009). Ağır metallerin bitkiler üzerine etkileri. Alinteri Journal of Agriculture Science 17(2): 14-26.
• Özay H (2013). Comparison study of low cost fly ash supported Cu, Co and Ni metal catalyst systems for the reduction of 4-nitrophenol. Science of Advenced Materials 5(6): 575-582.
• Öktüren FA, Sönmez S (2006). Ağır metal toksisitesinin bitki metabolizması üzerine etkileri. Derim 23(2): 36-45.
• Öner T (2006). Soya sektör raporu. İstatistik Şubesi 5(2017): 1-84.
• Özay C, Mammadov R (2013). Ağır metaller ve süs bitkilerinin fitoremediasyonda kullanılabilirliği. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 15(1): 68-77.
• Özbek K (2015). Hiperakümülasyon Ve Türkiye Florasındaki Hiperakümülatör Türler Kürşad Özbek. Toprak Bilimi Ve Bitki Besleme Dergisi 3(1): 37-43.
• Özkan G (2009). Endüstriyel bölge komşuluğunda kıyısal kırsal alandaki hava kalitesi; Muallimköy’de partikül maddede ve topraktaki ağır metal kirliliği (Yayımlanmamış Yüksek Lisans Tezi). GYTE Fen Bilimleri Enstitüsü, Gebze 9: 12-22.
• Poschenrieder C, Guns, B, Barcelo J (1989). Influence of cadmium on water relations, stomatal resistance and abscisic acid content in expanding bean leaves. Plant physiol 90: 1365-1371.
• Robinson B, Russell C, Hedley M, Clothier B (2001). Cadmium adsorption by rhizobacteria: implications for New Zealand pastureland. Agriculture, Ecosystems and Environment 87(3): 315-321.
• Safarzadeh S, Ronaghi A, Karimian N (2013). Effect of cadmium toxicity on micronutrient concentration, uptake and partitioning in seven rice cultivars. Archives Of Agronomy and Soil Science 59(2): 231-245.
• Sahin U, Ekinci M, Ors S, Turan M, Yıldız S, Yıldırım E (2018). Effects of individual and combined effects of salinity and drought on physiological, nutritional and biochemical properties of cabbage (Brassica oleracea var. capitata). Scientia Horticulturae 240: 196-204.
• Salt DE, Benhamou N, Leszczyniecka M, Raskin I, Chet I (1999). A possible role for rhizobacteria in water treatment by plant roots. International Journal of Phytoremediation 1(1): 67-79.
• Saygıdeğer S (1995). Lypcopersicum esculentum L. bitkisinin çimlenmesi ve gelişimi üzerine kurşunun etkileri. II. Ulusal Ekoloji ve Çevre Kongresi 588-597.
• Sharma P, Dubey RS (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology 17(1): 35-52.
• Sheoran IS, Aggarwal N, Singh R (1990). Effects of cadmium and nickel on in vivo carbon dioxide exchange rate of pigeon pea (Cajanus cajan L.). Plant and Soil 129(2): 243-249.
• Sönmez O, Kılıç FN (2021). Toprakta ağır metal kirliliği ve giderim yöntemleri. Turkish Journal of Agricultural Engineering Research 2(2): 493-507.
• Thuzar M, Puteh AB, Abdullah NAP, Lassim MBM, Jusaff K (2010). The effects of temperature stress o the quality and yield of soybean. Journal of Agricultare Science 2: 1-8.
• Tiryakioğlu M, Eker S, Ozkutlu F, Husted S, Cakmak I (2006). Antioxidant defense system and cadmium uptake in barley genotyes differing in cadmium tolerance. Journal of Trace Elements in Medicine and Biology 20(3): 181-189.
• Tunçtürk R, Tunçtürk M, Oral E (2021). Kuraklık stresi koşullarında yetiştirilen soya fasulyesinin (Glycine max L.) bazı fizyolojik özellikleri üzerine rizobacterium (PGPR) uygulamalarının etkisi. ÇOMÜ Ziraat Fakültesi Dergisi 9 (2): 359-368. DOI: 10.33202/comuagri.881226.
• Turhan S (2019). Farklı humik asit dozlarının soya (Glycine max L. merrill) çeşitlerinde verim ve kalite üzerine etkisi. Yüksek Lisans tezi, Iğdır Üniversitesi, Tarla Bitkileri Ana Bilim Dalı.
• Tüfekçi Ş (2019). Konya-Ereğli ticaret borsası 2019 AR-GE soya fasülyesi raporu, Ziraat mühendisi.
• TÜİK (2021). Türkiye İstatistik Kurumu. Web Sayfası. http://www.tuik.gov.tr (Erişim Tarihi 16.08.2022)
• Velikova V, Yordanov I, Edreva A (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant science 151(1): 59-66.
• Yerli C, Çakmakcı T, Sahin U, Tüfenkçi Ş (2020). Ağır metallerin toprak, bitki, su ve insane sağlığına etkileri. Türk Doğa ve Fen Dergisi 9 (özel sayı): 103-114.
• Yıldız N, Bircan H (1991). Araştırma ve deneme metotları. Atatürk Üniversitesi Yayınları.
• Yılmaz HŞ, Kökten K (2019). Kadmiyum (Cd) uygulamasının tane sorgumda (Sorghum bicolor L.) bazı morfolojik özellikler üzerine etkisinin belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi 6(3): 447-456.
• Yordanova RY, Christov KN, Popova LP (2004). Antioxidative enzymes in barley plants subjected to soil flooding. Environmental And Experimental Botany 51(2): 93-101.
• Zengin KF, Munzuroğlu Ö (2005). Fasulye fidelerinin (Phaseolus vulgaris L. Strike) klorofil ve karotenoid miktarı üzerine bazı ağır metallerin (Ni2+, Co2+, Cr3+, Zn2+) etkileri. FÜ Fen ve Mühendislik Bilimleri Dergisi 17(1): 164-172