soma myo teknik bilimler dergisi Soma Meslek Yüksekokulu Teknik Bilimler Dergisi 1304-6330 Manisa Celal Bayar University 10.47118/somatbd.1588884 Bioengineering (Other) Biyomühendislik (Diğer) Tip 2 Diyabet tedavisine yönelik Safranın Antidiyabetik Potansiyeli ve Araştırma Metodolojilerine Üzerine Derleme A Review on the Antidiabetic Potential of Saffron in the Treatment of Type 2 Diabetes Mellitus and Its Research Methodologies https://orcid.org/0009-0006-6740-1169 Sazlı Nil MANISA CELAL BAYAR UNIVERSITY https://orcid.org/0000-0002-8176-4883 Karataş Deniz MANISA CELAL BAYAR UNIVERSITY 12 31 2024 2 38 20 28 11 20 2024 12 13 2024 Copyright © 2004, Soma Vocational School Technical Sciences Journal 2004 Soma Vocational School Technical Sciences Journal

Bu çalışma Tip 2 Diyabet tedavisine yönelik Safran bitkisinin antidiyabetik potansiyeli ve araştırma metodolojileri üzerine bir derlemedir. Bu proje çalışmasının deneysel tasarımında, safran bitkisinden krosin elde edilmesi amaçlanmıştır. Krosin, yüksek antioksidan ve antidiyabetik özellikleri sayesinde potansiyel bir doğal ilaç etkeni olarak görülmektedir. Ancak, bu doğal etkeni elde etmede krosinin en yüksek verimini elde etmek için, doğru ekstraksiyon tekniklerini, fenolik içeriği, antioksidan ve antidiyabetik analizleri seçerek optimizasyonu sağlamak çok önemlidir. Bu projede, safran olarak da bilinen Crocus sativus bitkisinden krosin elde etmek için uygun koşullar altında ekstraksiyon tekniklerinin uygulanması amaçlanmıştır. Ayrıca, krosinin yüksek saflığını elde etmek için HPLC, toplam fenolik içeriğini belirlemek için TPC analizi, antioksidan kapasitesini belirlemek için ORAC ve DPPH analizleri ve antidiyabetik potansiyeli belirlemek için α-Amilaz İnhibisyon Testi ve α-Glukosidaz İnhibisyon Testi tekniklerini uygulamak üzere bir deneysel tasarım oluşturulmuştur. İstenilen sonuçlar elde edildiğinde, krosinin doğal bir pazar ilacı haline getirilmesi için deneysel çalışmalar başlatılacaktır.

This study is a review of the antidiabetic potential of the Saffron plant in the treatment of Type 2 Diabetes and research methodologies. The experimental design of this project study aimed to obtain crocin from the saffron plant. Crocin is considered a potential natural drug agent thanks to its high antioxidant and antidiabetic properties. However, in order to obtain the highest yield of crocin in obtaining this natural agent, it is very important to ensure optimization by selecting the right extraction techniques, phenolic content, antioxidant and antidiabetic analyses. In this project, it was aimed to apply extraction techniques under appropriate conditions to obtain crocin from the Crocus sativus plant, also known as saffron. In addition, an experimental design was created to apply HPLC to obtain high purity of crocin, TPC analysis to determine total phenolic content, ORAC and DPPH analyses to determine antioxidant capacity, and α-Amylase Inhibition Test and α-Glucosidase Inhibition Test techniques to determine antidiabetic potential. Once the desired results are achieved, experimental studies will be initiated to develop crocin into a natural market drug.

 Saffron Antidiabetic Natural Agent Crocin Medicine. Safran Antidiyabetik Doğal İlaç Krosin İlaç. El Midaoui A., Ghzaiel I., Vervandier-Fasseur D., Ksila M., Zarrouk A., Nury T., et al. (2022). "Saffron (Crocus sativus L.): A Source of Nutrients for Health and for the Treatment of Neuropsychiatric and Age-Related Diseases". Nutrients;14. Moini Jazani A., Karimi A., Nasimi Doost Azgomi R. (2022). "The potential role of saffron (Crocus Sativus L.) and its components in oxidative stress in diabetes mellitus: A systematic review". Clin Nutr ESPEN;48:148-157. Urbani E., Blasi F., Simonetti M.S., Chiesi C., Cossignani L. (2016). "Investigation on secondary metabolite content and antioxidant activity of commercial saffron powder". European Food Research and Technology;242:987-993. Hosseini A., Razavi B.M., Hosseinzadeh H. (2018). "Pharmacokinetic Properties of Saffron and its Active Components". Eur J Drug Metab Pharmacokinet;43:383-390. Maggi M.A., Bisti S., Picco C. (2020). "Saffron: Chemical Composition and Neuroprotective Activity". Molecules;25. Dhiman N., Kharkwal H. Biosynthesis and Derivatization of the Major Phytoconstituents of Saffron. Saffron2020. p. 83-92. Shoyama S. (2023). "Review on pharmacological active saffron and crocin". Medical Research Archives;11. Sani A., Tajik A., Seiiedi S.S., Khadem R., Tootooni H., Taherynejad M., et al. (2022). "A review of the anti-diabetic potential of saffron". Nutr Metab Insights;15:11786388221095223. Liu J., Yang Y., Qi Y. (2024). "Effect of saffron supplementation on the glycemic outcomes in diabetes: a systematic review and meta-analysis". Front Nutr;11:1349006. Hooshyari Ardakani M., Nosengo C., Felletti S., Catani M., Cavazzini A., De Luca C., et al. (2024). "Enhancing the purification of crocin-I from saffron through the combination of multicolumn countercurrent chromatography and green solvents". Anal Bioanal Chem;416:2553-2564. Siddiqui M.J., Saleh M.S.M., Basharuddin S., Zamri S.H.B., Mohd Najib M.H.B., Che Ibrahim M.Z.B., et al. (2018). "Saffron (Crocus sativus L.): As an Antidepressant". J Pharm Bioallied Sci;10:173-180. Ali A., Yu L., Kousar S., Khalid W., Maqbool Z., Aziz A., et al. (2022). "Crocin: Functional characteristics, extraction, food applications and efficacy against brain related disorders". Front Nutr;9:1009807. Salem M., Shaheen M., Tabbara A., Borjac J. (2022). "Saffron extract and crocin exert anti-inflammatory and anti-oxidative effects in a repetitive mild traumatic brain injury mouse model". Sci Rep;12:5004. Ronsisvalle S., Panico A., Santonocito D., Siciliano E.A., Sipala F., Montenegro L., et al. (2023). "Evaluation of Crocin Content and In Vitro Antioxidant and Anti-Glycation Activity of Different Saffron Extracts". Plants (Basel);12. Amatto P.P.G., Chaves L., Braga G.G., Carmona F., Pereira A.M.S. (2024). "Effect of Crocus sativus L. (saffron) and crocin in the treatment of patients with type-2 diabetes mellitus: A systematic review and meta-analysis". J Ethnopharmacol;319:117255. Shaheen M.J., Bekdash A.M., Itani H.A., Borjac J.M. (2021). "Saffron extract attenuates neuroinflammation in rmTBI mouse model by suppressing NLRP3 inflammasome activation via SIRT1". PLoS One;16:e0257211. Zaazaa L., Naceiri Mrabti H., Ed-Dra A., Bendahbia K., Hami H., Soulaymani A., et al. (2021). "Determination of Mineral Composition and Phenolic Content and Investigation of Antioxidant, Antidiabetic, and Antibacterial Activities of Crocus sativus L. Aqueous Stigmas Extracts". Adv Pharmacol Pharm Sci;2021:7533938. Mohs R.C., Greig N.H. (2017). "Drug discovery and development: Role of basic biological research". Alzheimers Dement (N Y);3:651-657. Peng X., Rao G., Li X., Tong N., Tian Y., Fu X. (2023). "Preclinical models for Type 1 Diabetes Mellitus - A practical approach for research". Int J Med Sci;20:1644-1661. Flores-Holguin N., Frau J., Glossman-Mitnik D. (2021). "Computational Pharmacokinetics Report, ADMET Study and Conceptual DFT-Based Estimation of the Chemical Reactivity Properties of Marine Cyclopeptides". ChemistryOpen;10:1142-1149. Najafi Z., Zahran H.A., Şahin Yeşilçubuk N., Gürbüz H. (2022). "Effect of different extraction methods on saffron antioxidant activity, total phenolic and crocin contents and the protective effect of saffron extract on the oxidative stability of common vegetable oils". Grasas y Aceites;73. Azarabadi N., Özdemir F. (2018). "Determination of Crocin Content and Volatile Components in Different Qualities of Iranian Saffron". Gıda;43:476-489.