Antimicrobial Resistance and Plasmid Typing of Coagulase Positive Staphylococcus Species Isolated from Bovine Mastitis
Abstract
In this study, a number of 50 Coagulase positive Staphylococcus isolates has been isolated and identified using conventional bacteriological methods from bovine mastitis. Of the total isolates 40 was identified as Staphylococcus aureus and 10 was identified as Staphylococcus intermedius. This study indicates the increasing significance of S.intermedius as the causative agents of bovine subclinical mastitis. All the Staphylococcus strains were examined for their susceptibilities to antibiotics and plasmid profiles. Plasmid profiling demonstrated that 38 of 40 S.aureus and 9 of 10 S.intermedius isolates contained plasmid. Molecular weight of plasmids varied from 1.1 to 19 kb. The highest resistance to chematerapotics used was observed to penicillin. This study suggests that plasmid profile analysis does not appear to be a suitable method for typing S.aureus and S.intermedius.
Mastitisli neklerden zole Edilen Koagulaz Pozitif Staphylococcus Türlerinin Antimikrobiyal Dirençlilii Ve Plazmit Tiplendirmesi
Abstract
Bu çalımada, 50 koagulaz pozitif Staphylococcus izolatı mastitisli ineklerden konventiyonal bakteriyolojik metodlar kullanılarak izole edilmi ve tanımlanmıtır. Toplam izolattan 40 tanesi Staphylococcus aureus olarak ve 10 tanesi ise Staphylococcus intermedius olarak tanımlanmıtır. Bu çalıma, subklinik mastitisli ineklerin etken ajanlar olarak S. intermedius’un öneminin arttıını göstermitir. Bütün Staphylococcus sularının antibiyotiklere duyarlılıkları ve plazmit profilleri incelenmitir. Plazmit profili 40 S. aureus izolatının 38’nin ve 10 S. intermedius izolatının 9’nun plazmit içerdiini göstermitir. Plazmitlerin moleküler aırlıkları 1.1 den 19 kb’a kadar deimektedir. Kullanılan kemoterapotiklere en yüksek dirençlilik penisilinde gözlenmitir. Bu çalıma, plazmit profili analizinin S. aureus ve S. intermedius’un tiplendirilmesi için uygun bir metod olmadıını göstermektedir.
Details
| Other ID | JA58MT34UK |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | June 1, 2004 |
| Submission Date | June 1, 2004 |
| Published in Issue | Year 2004 Volume: 1 Issue: 24 |
Cite
Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
It has been scanned in DergiPark since 2016.
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