Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus)

Tülin Aydemir; Demet Kavrayan; Seda Çınar

Year 2003, Volume: 1 Issue: 21, 115 - 125, 01.12.2003

Abstract

Polifenol oksidaz (PPO) enzimi yer elmasından izole edilmiştir. PPO, katekol, L-dopa, DL-dopa ya karşı aktivite göstermiş fakat bir monofenol olan L-tirozine karşı aktivite göstermemiştir. Yer elmasından izole edilen PPO nun optimum pH ve sıcaklık değeri sırasıyla 7.0 ve 25 °C dir. Enzim nötr pH civarında oldukça kararlıdır. 60 ve 80 °C deki aktivitenin % 50 inaktivasyonu için gerekli olan zaman sırasıyla, 40 ve 20 dakika olarak bulunmuştur. Katekol için Km ve Vmax değerleri sırasıyla, 5.88 mM ve 25 402 IU/mg.min dir. En çok inhibitör etkisi gösteren maddeler potasyum siyanid, β-merkaptoetanol ve tiyoüredir.

Keywords

Polifenol oksidaz, Yerelması, Helianthus tuberosus, izolasyon.

Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus)

Year 2003, Volume: 1 Issue: 21, 115 - 125, 01.12.2003

Tülin Aydemir Demet Kavrayan Seda Çınar

Abstract

Polyphenol oxidase (PPO) was isolated from Jerusalem artichoke (Helianthus tuberosus). PPO showed activity to catechol, L-dopa and DL-dopa, but the enzyme had no activity toward L-tyrosine. PH and temperature optima Jerusalem artichoke PPO were 7,0 and 25 °C. The enzyme was significantly stable at neutral pH region. The times required for 50 % inactivation of activity at 60 and 80 °C were found to be 40 and 20 min. respectively. Km and Vmax values were 5.88 mM and 25 402 IU/mg.min with catechol, respectively. Most effective inhibitors was found to be thiourea β-mercaptoethanol and potassium cyanide

Keywords

Polyphenol oxidase, Jerusalem artichoke (Helianthus tuberosus), characterisation, isolation, inhibitors

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Details

Other ID	JA58SH73JM
Journal Section	Research Articles
Authors	Tülin Aydemir This is me Demet Kavrayan This is me Seda Çınar This is me
Publication Date	December 1, 2003
Submission Date	December 1, 2003
Published in Issue	Year 2003 Volume: 1 Issue: 21

Cite

APA	Aydemir, T., Kavrayan, D., & Çınar, S. (2003). Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus). Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 1(21), 115-125.
AMA	Aydemir T, Kavrayan D, Çınar S. Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus). sufefd. December 2003;1(21):115-125.
Chicago	Aydemir, Tülin, Demet Kavrayan, and Seda Çınar. “Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus Tuberosus)”. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 1, no. 21 (December 2003): 115-25.
EndNote	Aydemir T, Kavrayan D, Çınar S (December 1, 2003) Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus). Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 1 21 115–125.
IEEE	T. Aydemir, D. Kavrayan, and S. Çınar, “Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus)”, sufefd, vol. 1, no. 21, pp. 115–125, 2003.
ISNAD	Aydemir, Tülin et al. “Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus Tuberosus)”. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi 1/21 (December 2003), 115-125.
JAMA	Aydemir T, Kavrayan D, Çınar S. Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus). sufefd. 2003;1:115–125.
MLA	Aydemir, Tülin et al. “Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus Tuberosus)”. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, vol. 1, no. 21, 2003, pp. 115-2.
Vancouver	Aydemir T, Kavrayan D, Çınar S. Isolation and Characterisation of Polyphenoloxidase from Jerusalem Artichoke (Helianthus tuberosus). sufefd. 2003;1(21):115-2.

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Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Hüseyin YILMAZ
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.

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