Can Lathyrus czeczottianus Bässler Be a New Source of Natural Antimutagenic Agents in Pharmacology? Evaluation from Mutagenic/Antimutagenic and Antimicrobial Perspectives

Year 2024, Volume: 50 Issue: 2, 35 - 42, 01.10.2024

Mustafa Kul , Ahmet Uysal

https://doi.org/10.35238/sufefd.1475894

Abstract

In this study, the mutagenicity/antimutagenicity and antimicrobial properties of methanol and water extracts of the aerial parts of the Lathyrus czeczottianus plant were investigated. After toxic dose determinations of the extracts were made, their mutagenic properties were evaluated by the Ames test (Salmonella/microsome). Mutagenicity studies with Salmonella typhimurium TA98 and TA100 strains were performed in the presence and absence of metabolic activation. Methanol and water extracts did not show any mutagenic effects at doses of 10000, 5000 and 1000 µg/plate. Antimutagenic inhibition rates against well-known mutagenic substances were evaluated. Accordingly, methanol and water extracts inhibited the effect of 2 aminofluorene at the highest doses in the presence of the S9 mixture by 73% and 85%, respectively, especially for the TA98 strain. In addition, methanol extract improved the effect of 2 aminoanthracene up to 78% in the presence of S9 for the TA100 strain and showed strong antimutagenicity. Antimicrobial activity studies were tested using the broth microdilution method against eight standard microorganisms and 14 methicillin-resistant Staphylococcus aureus isolates. Methanol and water extracts revealed low antimicrobial activity against the tested microorganisms at doses of 12.5 and 6.25 mg/ml. It was thought that L. czeczottianus extracts could be used as a natural chemo preventive agent against chemicals. This study is the first report on genotoxicity and antimicrobial properties on L. czeczottianus.

Keywords

Antimicrobial activity, Antimutagenicity, Ames test, Lathyrus czeczottianus, Mutagenicity

Lathyrus czeczottianus Bässler Farmakolojide Doğal Antimutajenik Ajanların Yeni Kaynağı Olabilir mi? Mutajenik/Antimutajenik ve Antimikrobiyal Açıdan Değerlendirme

Abstract

Bu çalışmada, Lathyrus czeczottianus bitkisinin toprak üstü kısımlarına ait metanol ve su özütlerinin mutajenite/antimutajenite ve antimikrobiyal özellikleri araştırılmıştır. Özütlerin toksik doz belirlemeleri yapıldıktan sonra, Ames testi (Salmonella/mikrozom) ile mutajenik özellikleri değerlendirilmiştir. Salmonella typhimurium TA98 ve TA100 suşları ile yapılan mutajenite çalışmaları metabolik aktivasyon varlığı ve yokluğunda gerçekleştirilmiştir. Metanol ve su öztüleri 10000, 5000 ve 1000 µg/plak dozlarda herhangi mutajenik etki göstermemiştir. Bilinen mutajenik maddelere karşı antimutajenik inhibisyon oranları değerlendirilmiştir. Buna göre metanol ve su özütleri özellikle TA98 suşu için 2 aminoflorenin S9 karışımı varlığında etkisini sırasıyla %73 ve %85 oranlarda en yüksek dozlarda inhibe etmiştir. Ayrıca metanol özütü TA100 suşu için S9 varlığında 2 aminoantrasenin etkisini %78’lere varan düzeyde iyileştirmiş ve güçlü antimutajenite göstermiştir. Antimikrobiyal aktivite çalışmaları ise sekiz standart mikroorganizma ve 14 metisilin dirençli Staphylococcus aureus izolatına karşı sıvı mikro dilüsyon yöntemiyle test edilmiştir. Metanol ve su özütleri test edilen mikroorganizmalara karşı 12.5 ve 6.25 mg/ml dozlarda düşük antimikrobiyal aktivite ortaya koymuştur. L. czeczottianus özütlerinin kimyasallara karşı kemopreventif doğal bir ajan olarak kullanılabileceği düşünülmüştür.

Keywords

Antimikrobiyal aktivite, Antimutajenite, Ames test, Lathyrus czeczottianus, Mutajenite

Thanks

Bitkinin toplanmasında ve özütlerin hazırlanmasında yardımlarını esirgemeyen Prof. Dr. Gökhan Zengin (Selçuk Üniversitesi) ve Dr. Ramazan Ceylan (Sabancı Üniversitesi)’a teşekkürlerimizi sunarız.

References

• Altundag, E. ve Ozturk, M., 2011, Ethnomedicinal studies on the plant resources of east Anatolia, Turkey, 2nd International Geography Symposium-Mediterranean Environment 2010, 19, 756-777.
• Birosova, L., Mikulasova, M. ve Vaverkova, S., 2005, Antimutagenic effect of phenolic acids, Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 149 (2), 489-491.
• Ceylan, R., Zengin, G., Guler, G. O. ve Aktumsek, A., 2021, Bioactive constituents of and ethyl acetate and water extracts and their biological activities: An endemic plant to Turkey, South African Journal of Botany, 143, 306-311.
• Chassagne, F., Samarakoon, T., Porras, G., Lyles, J. T., Dettweiler, M., Marquez, L., Salam, A. M., Shabih, S., Farrokhi, D. R. ve Quave, C. L., 2021, A Systematic Review of Plants With Antibacterial Activities: A Taxonomic and Phylogenetic Perspective, Frontiers in Pharmacology, 11.
• Choi, J. S., Park, K. Y., Moon, S. H., Rhee, S. H. ve Young, H. S., 1994, Antimutagenic effect of plant flavonoids in the Salmonella assay system, Archives of pharmacal research, 17, 71-75.
• Dean, B., Brooks, T., Hodson-Walker, G. ve Hutson, D., 1985, Genetic toxicology testing of 41 industrial chemicals, Mutation Research/Reviews in Genetic Toxicology, 153 (1-2), 57-77.
• Faydaoğlu, E. ve Sürücüoğlu, M. S., 2011, Geçmişten günümüze tıbbi ve aromatik bitkilerin kullanılması ve ekonomik önemi, Kastamonu University Journal of Forestry Faculty, 11 (1), 52-67.
• Geetha, T., Malhotra, V., Chopra, K. ve Kaur, I. P., 2005, Antimutagenic and antioxidant/prooxidant activity of quercetin, Indian Journal of Experimental Biology, 43 (1), 61-67.
• Gulluce, M., Agar, G., Baris, O., Karadayi, M., Orhan, F. ve Sahin, F., 2010, Mutagenic and antimutagenic effects of hexane extract of some Astragalus species grown in the eastern Anatolia region of Turkey, Phytotherapy Research, 24 (7), 1014-1018.
• Gulluce, M., Orhan, F., Adiguzel, A., Bal, T., Guvenalp, Z. ve Dermirezer, L. O., 2013, Determination of antimutagenic properties of apigenin-7-O-rutinoside, a flavonoid isolated from Mentha longifolia (L.) Huds. ssp. longifolia with yeast DEL assay, Toxicology and Industrial Health, 29 (6), 534-540.
• Günes, F. ve Meriç, Ç., 2017, Morphological, anatomical and karyological investigations of the Turkish endemic species Bornm. (Fabaceae), Acta Botanica Croatica, 76 (2), 132-137.
• Heydari, H., Iscan, G. S., Eryilmaz, M., Acikara, O. B., Sarialtin, S. Y., Tekin, M. ve Coban, T., 2019, Antimicrobial and Anti-Inflammatory Activity of Some Lathyrus L. (Fabaceae Species Growing in Turkey, Turkish Journal of Pharmaceutical Sciences, 16 (2), 240-245.
• Jakabfi-Csepregi, R., Alberti, A., Felegyi-Tóth, C. A., Köszegi, T., Czigle, S. ve Papp, N., 2024, A Comprehensive Study on L.: Insights into Phytochemical Composition, Antimicrobial Activity, Antioxidant Capacity, Cytotoxic, and Cell Migration Effects, Plants-Basel, 13 (2).
• Kenicer, G., 2005, Legumes of the World. Edited by G. Lewis, B. Schrire, B. MacKinder & M. Lock. Royal Botanic Gardens, Kew. 2005. , Edinburgh journal of botany, 62 (3), 195-196.
• Khan, N. A., Quereshi, S., Pandey, A. ve Srivastava, A., 2009, Antibacterial Activity of Seed Extracts of Commercial and Wild Species, Turkish Journal of Biology, 33 (2), 165-169.
• Lambein, F., Travella, S., Kuo, Y. H., Van Montagu, M. ve Heijde, M., 2019, Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?, Planta, 250 (3), 821-838.
• Llorent-Martínez, E. J., Zengin, G., Córdova, M. L. F. D., Bender, O., Atalay, A., Ceylan, R., Mollica, A., Mocan, A., Uysal, S., Guler, G. O. ve Aktumsek, A., 2017, Traditionally Used Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and Studies of L. czeczottianus and L. nissolia, Frontiers in Pharmacology, 8, 1-20.
• Manuja, R., Sachdeva, S., Jain, A. ve Chaudhary, J., 2013, A comprehensive review on biological activities of p-hydroxy benzoic acid and its derivatives, International Journal of Pharmaceutical Sciences Review and Research, 22 (2), 109-115.
• Maron, D. M. ve Ames, B. N., 1983, Revised methods for the Salmonella mutagenicity test, Mutation Research/Environmental Mutagenesis and Related Subjects, 113 (3-4), 173-215.
• Mohamed, S. M., 2009, Anthocyanins and fatty acids from the flowers of L. and their antimicrobial activity, Planta Medica, 75 (9), 1073-1074.
• Mortelmans, K. ve Zeiger, E., 2000, The Ames Salmonella/microsome mutagenicity assay, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 455 (1), 29-60.
• Nibras Qader Qader, I., Obali, I., Ismet Ucan, H., Uysal, A., Yilmaz Obali, A. ve Kul, M., 2022, Ortho-hydroxy bioactive schiff base compounds: Design, comprehensive characterization, photophysical properties and elucidation of antimicrobial and mutagenic potentials, Bioorganic Chemistry, 119, 105507.
• Roleira, F. M. F., Tavares-da-Silva, E. J., Varela, C. L., Costa, S. C., Silva, T., Garrido, J. ve Borges, F., 2015, Plant derived and dietary phenolic antioxidants: Anticancer properties, Food Chemistry, 183, 235-258.
• Sharifi-Rad, M., Iriti, M., Sharifi-Rad, M., Gibbons, S. ve Sharifi-Rad, J., 2016, Anti-methicillin-resistant (MRSA) activity of Rubiaceae, Fabaceae and Poaceae plants: A search for new sources of useful alternative antibacterials against MRSA infections, Cellular and Molecular Biology, 62 (9), 39-45.
• Spanou, C., Stagos, D., Tousias, L., Angelis, A., Aligiannis, N., Skaltsounis, A. L. ve Kouretas, D., 2007, Assessment of antioxidant activity of extracts from unique Greek varieties of plants using assays, Anticancer Research, 27 (5a), 3403-3410.
• Tosun, M., Uysal, A., Kursunlu, A. N. ve Guler, E., 2024, A new family of macrocyclic antibiotics based-on Pillar[5]arene concluding multi quinoline moieties, Tetrahedron, 151, 1-6.
• Uysal, A., Gunes, E., Sarikurkcu, C., Celik, H., Durak, Y. ve Uren, M. C., 2016a, New Prospective Materials for Chemoprevention: Three Phlomis, British Journal of Pharmaceutical Research, 10(3), 1-13.
• Uysal, A., Zengin, G., Mollica, A., Gunes, E., Locatelli, M., Yilmaz, T. ve Aktumsek, A., 2016b, Chemical and biological insights on Cotoneaster integerrimus: A new (-)- epicatechin source for food and medicinal applications, Phytomedicine, 23 (10), 979-988.
• Uysal, A., Ozer, O. Y., Zengin, G., Stefanucci, A., Mollica, A., Picot-Allain, C. M. N. ve Mahomoodally, M. F., 2019, Multifunctional approaches to provide potential pharmacophores for the pharmacy shelf: Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt, Computational Biology and Chemistry, 78, 64-73.
• Veiga, M., Costa, E. M., Silva, S. ve Pintado, M., 2020, Impact of plant extracts upon human health: A review, Critical Reviews in Food Science and Nutrition, 60 (5), 873-886.
• WHO, 2013, World Health Organization traditional medicine strategy: 2014-2023, World Health Organization, p. Yamada, J. ve Tomita, Y., 1996, Antimutagenic activity of caffeic acid and related compounds, Bioscience Biotechnology and Biochemistry, 60 (2), 328-329.
• Zengin, G., Uysal, A., Gunes, E. ve Aktumsek, A., 2014, Survey of Phytochemical Composition and Biological Effects of Three Extracts from a Wild Plant (Cotoneaster nummularia Fisch et Mey.): A Potential Source for Functional Food Ingredients and Drug Formulations, Plos One, 9 (11), e113527.