Molecular Characterization of Rose Genotypes (Rosa sp.) Based on RAPD-PCR

Year 2009, Volume: 18 Issue: 1-2, 36 - 42, 01.06.2009

Mikail Çalışkan Y Ağaoğlu

Abstract

The genetic variation among different 47 rose genotypes, consisting of 27 modern garden types, 15 cut - roses including one bud mutant genotype from Dallas cultivar and 5 old garden roses were identified by using RAPD (Random Amplified Polymorphic DNA) molecular technique. Within 176 fragments (150-2070 bp) 133 fragments resulted in 75.4% polymorphism generated by 19 selective primers tested in previous studies. RAPD-PCR analyses led to efficiently differentiation as tree main groups within 47 genotypes comprising of modern garden roses, cut roses and old garden roses, which have been confirmed by correlation by UPGMA algorithm (Unweighted Pair Group Method Using Arithmetic Averages) and dendogram was also formed according to their horticultural groups. The results indicated that RAPD method is one of the effective molecular tools for identification and assessing of genetic relationship within rose cultivars grown under different horticultural groups.

Keywords

Rosa sp.; RAPD; DNA isolation; genetic identification

Gül Genotiplerinin (Rosa sp.) RAPD-PCR Analizi ile Moleküler Düzeyde Tanımlanması

Abstract

Aralarındaki genetik varyasyonun belirlenmesine yönelik olarak, 27 modern bahçe tipi gül, Dallas kesme gül çeşidinden tomurcuk mutasyonu ile elde edilen bir genotip de dâhil olmak üzere 15 kesme gül çeşidi ve 5 eski bahçe gülü, RAPD (Random Amplified Polymorphic DNA) tekniği kullanılarak moleküler seviyede tanımlanmıştır. Analizler için seçilen 19 primer, uzunlukları 150-2070 bp arasında değişen 176 bant üretmiş ve bunların 133 adedinin polimorfik olduğu belirlenmiştir (% 75,4). UPGMA yöntemi kullanılarak elde edilen RAPD-PCR analizlerinin gülleri genotiplerini tanımlamada; yetiştiricilik amaçları farklı olan modern bahçe gülleri, kesme gül çeşitleri ve eski bahçe gülleri arasındaki genetik ilişkileri ortaya çıkarmada kullanılabileceği belirlenmiştir.

Keywords

Rosa sp.; RAPD; PCR; genetik tanımlama

References

• Ağaoğlu, Y.S., A. Ergül and Baydar, N. 2000. Molecular analysis of genetic diversity oil rose (Rosa damascena Mill.) grown Isparta (Turkey) region. Biotechnology Equipment, 14: 16-18.
• Atienza, S.G., A.M. Torres, T. Millan and J.I. Cubero, 2005. Genetic diversity in Rosa as revealed by RAPDs. Agriculturae Conspectus Scientificus, 70(3): 75-85.
• Baydar, N.G., H. Baydar and T. Debener, 2004. Analysis of genetic relationships among Rosa damascena plants grown in Turkey by using AFLP and microsatellite markers. Journal of Biotechnology, 111: 263-267.
• Baytop, T. 2001. Türkiye’de Eski Bahçe Gülleri. T.C. Kültür Bakanlığı Yayınları: 2593, Yayın Dairesi Başkanlığı Sanat Eserleri Dizisi: 319, Ankara.
• Broertjes, C. and A.M. Van Harten, 1978. Application of Mutation Breeding Methods on the Improvement of Vegetatively Propagated Crops. Elsevier, Amsterdam.
• Brown, N., S. Venkatasamy, G. Khittoo, T. Bahorun and S. Jawaheer, 2009. Evaluation of genetic diversity between 27 banana cultivars (Musa spp.) in Mauritius using RAPD markers. African Journal of Biotechnology, 8(9): 1834-1840.
• Cairns, T. 1993. Modern Roses 10. American Rose Society, Shreveport.
• Debener, T., C. Bartels and L. Mattiesch, 1996. RAPD analysis of genetic variation between a group of rose cultivars and selected wild rose species. Molecular Breeding, 2: 321–327.
• Doyle, J.J. and L.H. Doyle, 1988. Isolation of plant DNA from fresh tissue. Focus, 12(1): 13-15.
• Esselink, G., M. Smulders and B. Vosman, 2003. Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers. Theoretical and Applied Genetics, 106 (2): 277-286.
• Gaafar, R.M. and M.M. Saker, 2006. Monitoring of cultivars identity and genetic stability in strawberry varieties grown in Egypt. World Journal of Agricultural Sciences, 2(1): 29-36.
• Grossi, C., O. Raymond and M. Jay, 1997. Isozyme polymorphism of Rosa spp. and cultivar identification. Euphytica, 98: 11-19.
• Jan, C.H., D.H. Byrne, J. Manhart and H. Wilson, 1999. Rose germplasm analysis with RAPD markers. HortScience, 34(2): 341–345.
• Kaur, N., R.K. Sharma, D. Dhyani, S. Karthigeyan and P.S. Ahuja, 2007. Molecular characterization of interspecific hybrids of scented roses using RAPD markers. Acta Horticulturae, 751: 175-179.
• Kim, Y. and D.H. Byrne, 1994. Biosystematical classification of genus Rosa using isozyme polymorphism (abstract). HortScience, 29:483.
• Kruessmann, G. 1981. The Complete Book of Roses. Timber Press, Portland.
• Martin, M., F. Piola, M. Chessel and P. Heizmann, 2001. The domestication process of modern rose: genetic structure and allelic composition of rose complex. Theoretical and Applied Genetics, 102: 398-404.
• Nowbuth, P., G. Khittoo, T. Bahorun and S. Venkatasamy, 2005. Assessing genetic diversity of some Anthurium andraeanum Hort. cut-flower cultivars using RAPD Markers. African Journal of Biotechnology, 4(10): 1189-1194.
• Rajapakse, S., D.H. Byrne, L. Zhang, N. Anderson, K. Arumuganathan and R.E. Ballard, 2001. Two genetic linkage maps of tetraploid roses. Theoretical and Applied Genetics, 103: 575-583.
• Raymond, O., J.P. Biolley and M. Jay, 1995. Fingerprinting the selection process of ancient roses by means of floral phenolic metabolism. Biochemical Systematics and Ecology, 23: 555-565.
• Rholf, F.J. 1990. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System Version 1.8. Applied Biostatistics, New York.
• Rout, G., R. Kullu, J. Senapati, S.K. Aparajita and A. Mohapatra, 2007. Identification and genetic relationship among Polyscias and Schefflera (Araliaceae) using RAPD and ISSR markers. Plant Biotechnology, 24: 519-525.
• Sesli, M. and E.D. Yeğenoğlu, 2009. Genetic analysis on wild olives by using RAPD markers. African Journal of Agricultural Research, 4(8): 707-712.
• Torres, A.M., T. Millian and J.I. Cubero, 1993. Identifying rose cultivars using random amplified polymorphic DNA markers. HortScience, 28: 333-334.
• Walker, C.A. and D.J. Werner, 1997. Isozyme and randomly amplified polymorphic DNA (RAPD) analysis of Cherokee rose and its putative hybrids `Silver Moon' and `Anemone'. Journal of the American Society for Horticultural Science, 122: 659-664.
• Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18: 6531-6535.
• Zhang, D., E. Germain, S. Reynders-Aloisi and M.H. Gandelin, 2000. Development of amplified fragment length polymorphism markers for variety identification in rose. Acta Horticulturae, 508:113-122.