Molecular Characterization of Rose Genotypes (Rosa sp.) Based on RAPD-PCR

Yıl 2009, Cilt: 18 Sayı: 1-2, 36 - 42, 01.06.2009

Mikail Çalışkan , Y Ağaoğlu

Öz

The genetic variation among different 47 rose genotypes, consisting of 27 modern garden types, 15 cut - roses including one bud mutant genotype from Dallas cultivar and 5 old garden roses were identified by using RAPD (Random Amplified Polymorphic DNA) molecular technique. Within 176 fragments (150-2070 bp) 133 fragments resulted in 75.4% polymorphism generated by 19 selective primers tested in previous studies. RAPD-PCR analyses led to efficiently differentiation as tree main groups within 47 genotypes comprising of modern garden roses, cut roses and old garden roses, which have been confirmed by correlation by UPGMA algorithm (Unweighted Pair Group Method Using Arithmetic Averages) and dendogram was also formed according to their horticultural groups. The results indicated that RAPD method is one of the effective molecular tools for identification and assessing of genetic relationship within rose cultivars grown under different horticultural groups.

Anahtar Kelimeler

Rosa sp.; RAPD; DNA isolation; genetic identification

Gül Genotiplerinin (Rosa sp.) RAPD-PCR Analizi ile Moleküler Düzeyde Tanımlanması

Öz

Aralarındaki genetik varyasyonun belirlenmesine yönelik olarak, 27 modern bahçe tipi gül, Dallas kesme gül çeşidinden tomurcuk mutasyonu ile elde edilen bir genotip de dâhil olmak üzere 15 kesme gül çeşidi ve 5 eski bahçe gülü, RAPD (Random Amplified Polymorphic DNA) tekniği kullanılarak moleküler seviyede tanımlanmıştır. Analizler için seçilen 19 primer, uzunlukları 150-2070 bp arasında değişen 176 bant üretmiş ve bunların 133 adedinin polimorfik olduğu belirlenmiştir (% 75,4). UPGMA yöntemi kullanılarak elde edilen RAPD-PCR analizlerinin gülleri genotiplerini tanımlamada; yetiştiricilik amaçları farklı olan modern bahçe gülleri, kesme gül çeşitleri ve eski bahçe gülleri arasındaki genetik ilişkileri ortaya çıkarmada kullanılabileceği belirlenmiştir.

Anahtar Kelimeler

Rosa sp.; RAPD; PCR; genetik tanımlama

Kaynakça

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