The Efficacy of Native Entomopathogenic Nematodes against the Pine Processionary Moth, Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera: Thaumetopoeidae)

Yıl 2019, Cilt: 10 Sayı: 2, 118 - 126, 30.12.2019

Çiğdem Gözel , Uğur Gözel

https://doi.org/10.31019/tbmd.571191

Cited By: 2

Öz

The pine processionary moth, Thaumetopoea
pityocampa Den. &
Schiff. (Lepidoptera: Thaumetopoeidae), is one of the most important pests of
forest trees. In this study, the efficacy of native entomopathogenic nematodes
(EPNs) against the larvae of T.
pityocampa was investigated under laboratory conditions. Isolates of Steinernema
carpocapsae Weiser, S. feltiae Filipjev (Rhabditida:
Steinernematidae), Heterorhabditis bacteriophora (91) Poinar and H. bacteriophora (200) (Rhabditida: Heterorhabditidae) from
İstanbul, Sakarya and Çanakkale Provinces of Turkey were used in the study. Thaumetopoea pityocampa larvae were
collected from infested trees on the campus of Canakkale Onsekiz Mart University
in Canakkale Province. The efficacy assays were conducted with 1 larva and 200
infective juveniles (IJs) nematodes in a plastic petri dish at 10, 15 and 25 ^oC.
The larvae were checked daily for 7 days after inoculation with the IJs and the
mortalities were recorded. No mortality of T.
pityocampa larvae was observed on the first day. Depending on the nematode
species and the temperature, larval mortality ranged from 60% to 100%. Based on
the results of this study, there is considerable potential for the use of EPNs
for the biological control of T.
pityocampa.

Anahtar Kelimeler

Pine processionary moth, larva, entomopathogenic nematode, efficacy

Yerel Entomopatojen Nematodların Çam Kese Böceği Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera: Thaumetopoeidae)’ya Karşı Etkinliği

Öz

Çam kese böceği, Thaumetopoea pityocampa Den. &
Schiff. (Lepidoptera: Thaumetopoeidae) orman ağaçlarının en önemli
zararlılarından biridir. Bu çalışmada yerel entomopatojen nematodların (EPN) T. pityocampa larvalarına karşı
etkinlikleri laboratuvar koşullarında araştırılmıştır. Çalışmada Steinernema carpocapsae Weiser, S. feltiae Filipjev (Rhabditida: Steinernematidae), Heterorhabditis
bacteriophora (91) Poinar ve H.
bacteriophora (200) (Rhabditida: Heterorhabditidae) izolatları
kullanılmıştır. EPN’ler İstanbul, Sakarya ve Çanakkale illerinden, T. pityocampa larvaları ise Çanakkale
Onsekiz Mart Üniversitesi kampüs alanındaki zarar görmüş çam ağaçlarından elde
edilmiştir. Etkinlik denemeleri plastik petrilerde 10, 15 ve 25 ^oC’de,
200 IJs/T. pityocampa larva
yoğunluğunda yürütülmüştür. Petrilerdeki larvalar nematod inokulasyonundan
sonraki 7 gün boyunca kontrol edilmiş ve ölüm oranları kaydedilmiştir. İlk gün T. pityocampa larvalarında ölüm
görülmemiştir. Nematod türüne ve sıcaklığa bağlı olarak larvalardaki ölüm oranı %60-100 oranında değişmiştir. Çalışmadan elde edilen
sonuçlara göre, T. pityocampa’nın
biyolojik mücadelesinde EPN'lerin önemli bir kullanım potansiyeli vardır.

Anahtar Kelimeler

Pine processionary moth, larva, entomopathogenic nematodes, efficacy

Kaynakça

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