Year 2009, Volume 33 , Issue 3, Pages 181 - 188 2009-03-01

The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)
The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)

Ayper BOĞA [1] , Seçil BİNOKAY [2] , Yaşar SERTDEMİR [3]


This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA3), from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration (LC50), malformation (EC50), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. The corresponding LC50 and EC50 values determined for GA3 exposure were 1117.5 mg/L and 658 mg/L, respectively. The TI (LC50/EC50) value calculated for GA3 was 1.69. Different anomalies occurred in the embryos, depending on the GA3 concentration. Based on these results, we conclude that GA3 is toxic and teratogenic to Xenopus laevis embryos. Moreover, our results confirm that the FETAX assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in agriculture.
This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA3), from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration (LC50), malformation (EC50), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. The corresponding LC50 and EC50 values determined for GA3 exposure were 1117.5 mg/L and 658 mg/L, respectively. The TI (LC50/EC50) value calculated for GA3 was 1.69. Different anomalies occurred in the embryos, depending on the GA3 concentration. Based on these results, we conclude that GA3 is toxic and teratogenic to Xenopus laevis embryos. Moreover, our results confirm that the FETAX assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in agriculture.
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Primary Language tr
Journal Section Articles
Authors

Author: Ayper BOĞA

Author: Seçil BİNOKAY

Author: Yaşar SERTDEMİR

Dates

Publication Date : March 1, 2009

Bibtex @ { tbtkbiology139864, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {}, publisher = {TUBITAK}, year = {2009}, volume = {33}, pages = {181 - 188}, doi = {}, title = {The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)}, key = {cite}, author = {BOĞA, Ayper and BİNOKAY, Seçil and SERTDEMİR, Yaşar} }
APA BOĞA, A , BİNOKAY, S , SERTDEMİR, Y . (2009). The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX). Turkish Journal of Biology , 33 (3) , 181-188 . Retrieved from https://dergipark.org.tr/en/pub/tbtkbiology/issue/11712/139864
MLA BOĞA, A , BİNOKAY, S , SERTDEMİR, Y . "The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)". Turkish Journal of Biology 33 (2009 ): 181-188 <https://dergipark.org.tr/en/pub/tbtkbiology/issue/11712/139864>
Chicago BOĞA, A , BİNOKAY, S , SERTDEMİR, Y . "The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)". Turkish Journal of Biology 33 (2009 ): 181-188
RIS TY - JOUR T1 - The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX) AU - Ayper BOĞA , Seçil BİNOKAY , Yaşar SERTDEMİR Y1 - 2009 PY - 2009 N1 - DO - T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 181 EP - 188 VL - 33 IS - 3 SN - 1300-0152-1303-6092 M3 - UR - Y2 - 2020 ER -
EndNote %0 Turkish Journal of Biology The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX) %A Ayper BOĞA , Seçil BİNOKAY , Yaşar SERTDEMİR %T The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX) %D 2009 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 33 %N 3 %R %U
ISNAD BOĞA, Ayper , BİNOKAY, Seçil , SERTDEMİR, Yaşar . "The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)". Turkish Journal of Biology 33 / 3 (March 2009): 181-188 .
AMA BOĞA A , BİNOKAY S , SERTDEMİR Y . The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX). Turkish Journal of Biology. 2009; 33(3): 181-188.
Vancouver BOĞA A , BİNOKAY S , SERTDEMİR Y . The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX). Turkish Journal of Biology. 2009; 33(3): 188-181.