Investigation of the presence of Mycoplasma as an etiologic agent of inflammatory airway diseases in thoroughbred racehorses in İstanbul Province

Year 2017, Volume 41, Issue 3, 365 - 371, 12.06.2017

Alper METE Naciye Yakut ÖZGÜR

Abstract

Mycoplasma equirhinis and Mycoplasma felis are thought to be infectious etiologic agents of inflammatory airway disease (IAD), which, after musculoskeletal injuries, is the second most common cause of poor performance in thoroughbred racehorses. The aims of this study were to investigate for the first time the presence of M. equirhinis, M. felis, and other Mycoplasma spp. in the lower respiratory tract of racehorses in İstanbul Province in Turkey and to statistically evaluate the association between clinical signs of IAD and the presence of mycoplasmas. Tracheal wash (TW) samples were collected from 111 English and Arabian thoroughbred horses that showed clinical signs of IAD. TW samples were examined by culture and PCR methods. The detection rates of Mycoplasma spp., M. felis, and M. equirhinis were found to be 16.2%, 0%, and 18% by culture and 59.5%, 1.8%, and 6.3% by PCR in TW samples, respectively. According to statistical evaluations, a significant association was found for the presence of M. felis in English thoroughbred racehorses. However, no significant association was found between clinical signs of IAD and the presence of M. equirhinis, M. felis, and other spp.

Keywords

Mycoplasma spp., Mycoplasma equirhinis, Mycoplasma felis, inflammatory airway disease

Abstract

References

• Chanter N. Bacterial Infections Including Mycoplasmas. Ithaca, NY, USA: International Veterinary Information Service; 2002.
• Couëtil LL, Cardwell JM, Gerber V, Lavoie JP, Léguillette R, Richard EA. Inflammatory airway diseases of horses-revised consensus statement. J Vet Intern Med 2016; 30: 503-515.
• Wood JLN, Newton JR, Chanter N, Mumford JA. Association between respiratory disease and bacterial and viral infections in British racehorses. J Clin Microbiol 2005; 43: 120-126.
• Cardwell JM, Smith KC, Wood JLN, Newton JR. A longitudinal study of respiratory infections in British national hunt racehorses. Vet Rec 2013; 172: 637.
• Wood JLN, Newton JR, Chanter N, Mumford JA. Inflammatory airway disease, nasal discharge and respiratory infections in young British racehorses. Equine Vet J 2005; 37: 236-242.
• Hodgson JL, Hodgson DR. Inflammatory Airway Disease. Ithaca, NY, USA: International Veterinary Information Service; 2002.
• Newton JR, Wood JLN, Chanter N. A case control study of factors and infections associated with apparent respiratory disease in UK thoroughbred racehorse. Prev Vet Medi 2003; 60: 107-132.
• Wood JLN, Newton JR, Burrell MH, Dugdale D, Windsor HM, Windsor GD, Rosendal S, Townsend HGG. An outbreak of respiratory disease in horses associated with Mycoplasma felis infection. Vet Rec 1997; 140: 388-391.
• Aydın N, İzgür M, Diker KS, Yardımcı H, Esendal Ö, Paracıklıoğlu J, Akan M. Veteriner Mikrobiyoloji (Bakteriyel Hastalıklar). Ankara, Turkey: İlke Emek Yayınları; 2006 (in Turkish).
• Queen PJ, Carter ME, Markey BK, Carter GR. Clinical Veterinary Microbiology. 1st ed. Dublin, Ireland: Wolfe Publishing; 1994. Allam NM, Lemcke RM. Mycoplasmas isolated from respiratory tract of horses. J Hyg-Camb 1975; 74: 385.
• Clark C, Greenwood S, Boison JO, Trejo MC, Dowling PM. Bacterial isolates from equine infections in western Canada (1998-2003). Can Vet J 2008; 49: 153-160.
• Poland P, Lemcke R. Mycoplasmas of the respiratory tract of horses and their significance in upper respiratory tract disease. In: Proceedings of the 4th International Conference on Equine Infectious Diseases. Princeton, NJ, USA: ICEID; 1978. pp. 437- 446.
• Robinson C. HBLB Funded Project Ref. 697. HBLB Veterinary Newsletter 2006; 10-1: 2.
• Bonilla HF, Chenoweth CE, Tully JG, Blythe LK, Robertson JA, Ogenovski VM, Kaufmann CA. Mycoplasma felis septic arthritis in a patient with hypogammaglobulinemia. Clini Infect Dis 1997; 24: 222-225.
• Carman S, Rosendal S, Huber L, Gyles C, McKee S, Willoughby A, Dubovi E, Thorsen J, Lein D. Infectious agents in acute respiratory disease in horses in Ontario. J Vet Diagn Invest 1997; 9: 17-23.
• Dixon PM. Ancillary diagnostic techniques for the investigation of equine pulmonary disease. Equine Vet Educ 1997; 9: 72-80.
• Laus F, Attili AR, Cerquetella M, Spaterna A, Tessei B, Cutteri V. Endoscopic findings, microbiological and cytological evaluation of tracheal aspirates in a population of Standardbred horses with poor performances. Vet Medi Praha 2009; 9: 444- 450.
• Christley RM, Hodgson DR, Rose RJ, Wood JLN, Reid SWJ, Whitear KG, Hodgson JL. A case-control study of respiratory disease in thoroughbred racehorses in Sydney, Australia. Equine Vet J 2001; 33: 256-264.
• Moorthy ARS, Spradbrow PB. Isolation of mycoplasmas from the respiratory tract of horses in Australia. Vet Rec 1976; 98: 235-237.
• Van Kuppeveld FJ, Johansson KE, Galama JM, Kissing J, Bolske G, van der Logt JT, Galama MD, Melchers WJ. Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Appl Environ Microbiol 1994; 60: 149- 152.
• Chalker VJ, Owen WMA, Paterson CJI, Browlie J. Development of a polymerase chain reaction for the detection of Mycoplasma felis in domestic cats. Vet Microbiol 2004; 100: 77-82.
• Akdamar K. SPSS ile Biyoistatistik. 3. Baskı. Eskişehir, Turkey: Kaan Kitapevi; 1999 (in Turkish).
• Allam NM, Powell DG, Andrews BE, Lemcke RM. The isolation of Mycoplasma species from horses. Vet Rec 1973; 93: 402.