Farklı Hematopoetik Kök Hücre Kaynaklarının Koloni Colony Forming Unit-Assay Oluşturma Kapasitelerinin Karşılaştırılması

Year 2011, Volume: 5 Issue: 4, 197 - 201, 01.04.2011

Meltem Özgüner Betül T Avil Yasin Köksal Elif Canal İkbal Ok Bozkaya Bahattin Tunç

Abstract

Giriş ve Amaç: Günümüzde Hematopoetik Kök Hücre Nakli (HKHN) özellikle hematolojik malignitelerin tedavisinde başarıyla uygulanmaktadır. Hematopoetik kök hücre (HKH) kaynağı olarak allojenik nakillerde genellikle direkt kemik iliğinden, otolog nakillerde ise genellikle kemik iliğinden mobilize edilip periferik kandan elde edilen hematopoetik kök hücreler kullanılmaktadır. Bu çalışmada farklı kaynaklardan elde edilen hematopoetik kök hücrelerin koloni oluşturma kapasitelerinin karşılaştırılması amaçlandı.Gereç ve Yöntemler: Hematopoetik kök hücre örnekleri 7 olguda direk kemik iliğinden, 7 olguda da periferik kandan elde edildi. Elde edilen hematopoetik kök hücrelerle invitro hücre kültür ortamlarına alındı. Periferik kan ve kemik iliği kaynaklı HKH örnekleri uygun Semi-Solid Agar besiyerinde (Methocult, H 4434 Classic, Stem Cell Technologies, Canada) kültüre edilerek 14-18 gün sonra koloni sayıları belirlenerek Colony Forming Unit Assay (CFU-Assay) hesaplandı.Bulgular: Otolog 7 vericiden alınan periferik kan HKH örneklerinin koloni sayımlarının ortalamaları CFU-GM:28.44x104/kg, BFU-E:40.7x104/kg, CFU-E:0.79x104/kg, CFU-GEMM :1.95x104/kg. Allojenik 7 vericiden alınan kemik iliği HKH örneklerinin Koloni sayımlarının ortalamaları CFU-GM:7.03x104/kg, BFU-E:10.46x104/kg, CFU-E:0.16x104/kg, CFU-GEMM :0.51x104/kg.Sonuç: CFU-Assay ile değerlendirilen periferik kan kaynaklı HKH kolonilerinin sayısı kemik iliği kaynaklı HKH kolonilere göre daha yüksek bulundu. Hematopoetik kök hücre nakillerinde özellikle erişkinlerde periferik kan kaynaklı hematopoetik kök hücrelerin kullanımı giderek yaygınlaşmaktadır. Pediatrik grupta da klinik verilerle invitro hücre kültür bulgularının desteklenmesi periferik kan kaynaklı HKH kullanımı için tercih sebebi olabilir.

Keywords

Kök hücre, CFU-Assay, hematopoetik kök hücre nakli

COMPARISON OF COLONY FORMING UNIT-ASSAY RESULTS OF DIFFERENT HEMATOPOIETIC STEM CELL SOURCES

Abstract

Aim: Hematopoietic stem cell transplantation (HSCT) is practiced successfully especially for hematologic malignancies. The source of hematopoietic stem cells for HSCT is bone marrow in allogeneic transplantation or peripheral blood stem cells after mobilization in autologous transplantation. The aim of this study is to investigate CFU-ASSAY results of two different hematopoietic stem cell sources.Method: Hematopoietic stem cells collected from bone marrow in 7 patients and peripheral blood in 7 patients were included in the study. Hematopoietic stem cells were cultured in semi-solid agar culture medium (Methocult, H 4434 Classic, Stem Cell Technologies, Canada) and CFU-ASSAY was performed after 14-18 day of culture period for each group.Results: CFU-Assay results of peripheral blood stem cells for autologous donors were as follows; CFUGM:28.44x104/kg, BFU-E:40.7x104/kg, CFU-E:0.79x104/kg, CFU-GEMM :1.95x104/kg. CFU-Assay results of bone marrow stem cells for allogeneic donors were as follows; CFU-GM:7.03x104/kg, BFUE:10.46x104/kg, CFU-E:0.16x104/kg, CFU-GEMM:0.51x104/kg.Conclusion: CFU-Assay results revealed that colony forming capacity of peripheral blood stem cells are higher than that of bone marrow stem cells. Peripheral blood stem cells transplantation is becoming more prevalent especially in adults. In pediatric groups, to identify the correlation between invitro results and clinical observations may help to increase preferance of peripheral blood stem cell transplantation

Keywords

Stem Cell, CFU-Assay, hematopoietic stem cell transplantation

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