Bazı metal iyonlarının tavuk böbreğinden saflaştırılan glutatyon redüktaz enzimi üzerine in vitro etkilerinin araştırılması

Year 2017, Volume: 6 Issue: 1, 24 - 30, 05.10.2017

Yusuf Karagözoğlu , Mehmet Çiftci

Abstract

Glutayon redüktaz enzimi tavuk böbreğinden
saflaştırıldı ve bazı metal iyonlarının bu enzim aktivitesi üzerine in vitro
etkileri araştırıldı. Saflaştırma prosedürü üç basamaktan oluştu; homojenatın
hazırlanması, amonyum sülfat çöktürmesi ve affinite kromatografisi. Bu üç
basamak sonucunda 8,595 EÜ/mg protein spesifik aktivitesine sahip enzim ~ % 57
verimle 369 kat saflaştırıldı. Enzimin saflık kontrolü sodyum dodesil
sülfat-poliakrilamid jel elektroforezi (SDS-PAGE) ile yapıldı ve bazı metal
iyonlarının (Ni⁺², Zn⁺², Pb⁺², Hg⁺²,
Ag⁺ and Al⁺³) glutatyon redüktaz enzimi üzerindeki
inhibisyon etkisi araştırıldı. Metal iyonları için K_i ve IC₅₀
değerleri Lineweaver-Burk
ve % vs [I] aktivite grafikleriyle belirlendi. Ni⁺²,  Hg⁺²
ve Ag⁺ yarışmasız inhibisyon gösterirken, Zn⁺² ve Pb⁺²
yarı yarışmalı inhibisyon gösterdi. Diğer metal iyonlarının aksine Al⁺³
enzim üzerinde aktivatör etkisi gösterdi.

Keywords

Glutatyon redüktaz, tavuk böbreği, metal iyonları

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