In Vitro Koşullarda Değişik Konsantrasyonlarda Ağır Metaller İçeren Besin Ortamlarının Kabak (Cucurbita pepo L.) Bitkisi Gelişimi Üzerine Etkileri

Year 2023, Volume: 12 Issue: 1, 30 - 36, 27.03.2023

Zeki Mancak Gökhan Baktemur

https://doi.org/10.46810/tdfd.1225384

Abstract

Kabak (Cucurbita pepo L.), dünyada ve ülkemizde sevilerek tüketilen bir sebzedir.
Yetiştiriciliği, hem açıkta hem de örtüaltında yapılmaktadır. Ağır metaller, toksik etki yayması
sebebiyle tüm canlılar için olumsuz durumlar oluşturmaktadırlar. Bu çalışmada, in vitro koşullar
altında değişik konsantrasyonlarda (0, 100, 200, 300, 400 ve 500 μM) hazırlanmış alüminyum (Al),
kadmiyum (Cd), kobalt (Co), kurşun (Pb) ve nikel (Ni) ağır metalleri Murashige ve Skoog (MS)
besin ortamına eklenerek kabak tohumları kültüre alınmıştır. Araştırmada yaprak ve kök sayısı,
gövde ve kök uzunlukları tespit edilmiştir. Yaprak sayısı tüm ağır metaller için kontrol grubunda en
fazla olurken, ağır metal konsantrasyonu arttıkça kök sayıları azalmaya başlamıştır. Gövde uzunluğu
ağır metallerin tamamında kontrol grubunda en fazla sayıya ulaşırken, kök uzunlukları 400 ve 500
μM dozlarında en düşük seviyeleri görmüştür. Bu sonuçlar dikkate alındığında, ağır metal dozu
arttıkça bitki gelişiminin gerilediği ortaya çıkmıştır.

Keywords

Ağır metal, Cucurbita pepo L., Bitki doku kültürü, Tohum

Effects of Nutrient Media Including Heavy Metals at Different Concentrations in In Vitro Conditions on the Growth of Squash (Cucurbita pepo L.)

Abstract

Squash (Cucurbita pepo L.) is a vegetable species that is appreciated and consumed in
the Türkiye world and in the world. Its cultivation is carried out both in the open field and
greenhouse. Heavy metals create adverse conditions for all living things due to their toxic effects.
In this study, key heavy metals like aluminum (Al), cadmium (Cd), cobalt (Co), lead (Pb) and nickel
(Ni) were added to the nutrient medium (Murashige and Skoog-MS)at different concentrations (0,
100, 200, 300, 400 and 500 μM). Squash seeds were germinated under in vitro conditions. During
the research, the number of leaves and roots, stem and root lengths were determined. According to
the results, the number of leaves was the highest in the control group for all heavy metals. The
number of roots decreased as the heavy metal concentration increased. While stem length reached
the highest number in all heavy metals in the control group, root lengths had the lowest levels at 400
and 500 μM doses. Considering these results, it has been revealed that as the dose of heavy metals
increases, the growth of the plant is retarded.

Keywords

Heavy metals, Cucurbita pepo L., Plant tissue culture, Seed

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