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Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province

Year 2024, Volume: 13 Issue: 1, 1 - 6, 26.03.2024
https://doi.org/10.46810/tdfd.1266565

Abstract

The cucumber plant (Cucumis sativus L) is an important cultivated plant produced worldwide. Cucumber mosaic virus (Cucumber mosaic virus, CMV), one of the common viral diseases, causes economic losses by reducing the yield and quality of the cucumber plant. In the observations performed in Diyarbakır in September 2021, cucumber plants showing virus-like symptoms such as mosaic, irregular yellowish spots, and deformity on the leaves were observed. Symptomatic and non-symptomatic samples were collected and subjected to a polymerase chain reaction (PCR) using CMV-specific primers, and the produced DNA bands were visualized on an agarose gel. CMV infection was detected in seven of the 15 samples. Bacterial cloning and sequencing of a randomly selected specimen determined that the CMV partial coat protein gene was 593 bp long and was registered in the NCBI database with the accession number MW962979.1. According to the phylogenetic tree performed with different isolates of CMV, Diyarbakir CMV isolate clustered with CMV isolates from Australia, Israel, Spain, Hungary, Japan, and Korea forming Subgroup IA. The presence of CMV and group/subgroup diagnosis in cucumber plants grown in the Diyarbakır region were confirmed molecularly for the first time by this study.

References

  • Doolittle SP. A new infectious mosaic disease of cucumber. Phytopathology. 1916; 6(2):145-147.
  • Adams IP, Glover RH, Monger WA, Mumford R, Jackeviciene E, Navalinskiene M, Samuitiene M, Boonham N. Next-generation sequencing and metagenomic analysis: A universal diagnostic tool in plant virology. Molecular Plant Pathology. 2009; 10(4):537-545.
  • Edwardson JR, Christie RG. Cucumoviruses. In: Edwardson JR, Christie RG, editors. CRC handbook of viruses infecting Legumes. CRC Press: Boca Raton; 1991. p. 293-319.
  • Jacquemond M. Cucumber mosaic virus. Advances in Virus Research. 2012; 84:439-504.
  • Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RI. Cucumber mosaic virus. Advances in Virus Research. 1992; 41:281-348.
  • Roossinck MJ, Zhang L, Hellwald KH. Rearrangements in the 5' nontranslated region and phylogenetic analyses of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. Journal of Virology. 1999; 73(8):6752-6758.
  • Lecoq, H., and Desbiez, C. Virus of cucurbit crops in the Mediterranean Region: an ever-changing picture. In: Loebenstein G, Lecoq H, editors. Viruses and Virus Diseases of Vegetables in the Mediterranean Basin. Virus Research; 2012. p. 67-126.
  • Mijatovic M, Zdravkovic J, Markovic Z, Obradovic A. Disease intensity of some tomato viruses in Serbia. Acta Physiologiae Plantarum. 2000; 22(3):332-335.
  • Arlı-Sökmen M, Mennan H, Şevik MA, Ecevit O. Occurrence of Viruses in Field grown Pepper Crops and Some of Their Reservoir Weed Hosts in Samsun, Turkey. Phytoparasitica. 2005; 33:347-358.
  • Çağlar BK. Characterization of melon (CMV-K), tomato (CMV-D), pepper (CMV-B) isolates of cucumber mosaic virus (CMV) by biological, serological, molecular methods and effect of satellite-RNAs on virus. Adana: Çukurova University, 2006.
  • Berniak H, Kaminska M, Malinowski T. Cucumber mosaic virus groups IA and II are represented among isolates from naturally infected lilies. European Journal of Plant Pathology. 2010; 127:305-309.
  • Temfack D, Dély C, Chofong G, Mbulli A, Gentil I, Ouboum F, et al. Detection of Cucumber mosaic virus on Solanum lycopersicum L. and Capsicum annuum L. in Western region of Cameroon. Journal of Agriculture and Food Research. 2022; 8:100294.
  • Foissac X, Svanella-Dumas L, Dulucq MJ, Candresse T, Gentit P. Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). Acta Hortic. 2001; 550:37-43.
  • Günay A. The investigation of some tobacco viruses in tobacco fields of Adıyaman by multiplex RT-PCR method and the molecular characterization of some virus isolates. Van: Van Yüzüncü Yıl University; 2019.
  • Raccah B. Detection of virus diseases by advanced techniques and control. Epidemiology and control of cucurbit viruses in Israel. 1. Israeli- Turkish Workshop. Turkey: Adana; 1999. p. 45-46.
  • Kaya A, Erkan S. Detection and incidence of viruses in cucurbits grown in Izmir, Aydın, Manisa and Balıkesir provinces. Plant Protection Bulletin. 2011; 51(4):387-405.
  • Palukaitis P, Garcia-Arenal F. Cucumber mosaic virus [Internet], Description of Plant Viruses, 2003: No; 400 [cited 2023 February 20]. Available from: https://www.dpvweb.net/dpv/showdpv/?dpvno=400
  • Gümüş M, Erkan S, Tok S. Studies on determination of virus diseases in the seeds of some Cucurbitaceous species. Ege Üniversitesi Ziraat Fakültesi Dergisi. 2004; 41(1):49-56.
  • Çulal Kılıç H, Yardımcı N. Cucumber mosaic Virus in Bean Growing Areas of Çine Plain, Burdur, Turkey. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2012; 3(2):12-15.
  • Ergün M, Semih E, Paylan IC. Cucumber mosaic virus in globe artichoke in Turkey. Canadian Journal of Plant Pathology. 2013; 35(4):514-517.
  • Erkan S, Gümüş M, Paylan İC, Duman İ, Ergün M. The determination of viral agents in certain cold-season vegetables in İzmir province and its around. Ege Üniversitesi Ziraat Fakültesi Dergisi. 2013; 50(3):311-322.
  • Uzunoğulları N, Gümüş M. Detection of cucumber mosaic virus (CMV) causing natural infection on some cultured plants in Marmara Region. Trakya University Journal of Natural Sciences. 2015; 16(1):9-15.
  • Gökdağ S, Karanfil A, Korkmaz S. Çanakkale ili ıspanak alanlarındaki Şalgam mozaik virüsü ve Hıyar mozaik virüsü varlığının belirlenmesi. Bahçe. 2016; özel sayı (cilt II):166-170.
  • Güngör M, Uzunbacak H, Kutluk-Yilmaz ND, Şevik MA. Determination of viruses causing infection in spinach fields in Samsun province. Anadolu Journal of Agricultural Sciences. 2017; 32(2):164-168.
  • Katul L, Makkouk KM. Occurrence and serological relatedness of five cucurbit potyviruses in Lebanon and Syria. Phytopathologia Mediterranea. 1987; 26(1):36-42.
  • Tobias I, Szabo B, Salanki K, Sari L, Kuhlmann H, Palkovics L. Seed borne transmission of Zucchini yellow mosaic virus and Cucumber mosaic virus in Styrian Hulless group of Cucurbita pepo. Proceedings of the IX EUCARPA Meeting of Genetics and Plant Breeding of Cucurbitaceae. France: Avignon; 2008. p. 189-197.
  • Karanfil A. Prevalence and molecular characterization of Cucumber mosaic virus isolates infecting tomato plants in Marmara region of Turkey. Plant Protection Bulletin. 2021; 61(4):19-25.
  • Yardimci N, Eryigit H. Identification of Cucumber mosaic virus in tomato (Lycopersicon esculentum) growing areas in the north-west Mediterranean region of Turkey. New Zealand Journal of Crop and Horticultural Science. 2006; 34(2):173-175.
  • Güller A, Usta M. Occurrence of Cucumber mosaic cucumovirus and Watermelon mosaic potyvirus on Melon exhibiting viral symptoms in Bingöl province of Turkey and their phylogenetic affinities. Türk Tarım ve Doğa Bilimleri Dergisi. 2020;7(4):948-958.
  • Öztürk S. Survey on the virus diseases of watermelon in Diyarbakır and districts. Adana: Çukurova University; 2000.
  • Budak E. Determination and incidence of viruses in cucurbits grown in Diyarbakır and its districts. Şanlıurfa: Harran University; 2015.
  • Güneş N, Gümüş M. Detection and characterization of tomato spotted wilt virus and cucumber mosaic virus on pepper growing areas in Antalya. Journal of Agricultural Sciences. 2019;25(3):259-271.
  • Karanfil A, Korkmaz S. Detection and molecular characterization based on coat protein gene of Cucumber mosaic virus (CMV) from cowpea production fields of Çanakkale province in Turkey. Plant Protection Bulletin. 2017; 57(3):293-304.
  • Ohshima K, Matsumoto K, Yasaka R, Nishiyama M, Soejima K, Korkmaz S, et al. Temporal analysis of reassortment and molecular evolution of cucumber mosaic virus: Extra clues from its segmented genome. Virology. 2016; 487:188-197.
  • Chen S, Gu H, Wang X, Chen J, Zhu W. Multiplex RT-PCR detection of Cucumber mosaic virus subgroups and Tobamoviruses infecting Tomato using 18S rRNA as an internal control. Acta Biochim Biophys Sin. 2011; 43(6), 465-471.
  • Balsak SC, Kardeş H, Buzkan N. First report of cucumber mosaic virus in cyclamen (Cyclamen spp.) in Turkey. Journal of Plant Pathology, 2021; 103 (1), 361-362.
  • Kurtoğlu A, Korkmaz S. Determination and molecular characterization of cucumber mosaic virus (CMV) infection on spinach production fields of Çanakkale province, Turkey. The Journal of Turkish Phytopathology, 2018; 47 (2), 43-51.
  • Özdemir S, Erilmez S. First report of Alfalfa mosaic virus and Cucumber mosaic virus in pepino in Turkey. Journal of Plant Pathology, 2012; 94 (4), 91.
  • Çağlar BK. Characterization of melon (CMV-K), tomato (CMV-D), pepper (CMV-B) isolates of cucumber mosaic virus (CMV) by biological, serological, molecular methods and effect of satellite-RNAs on virus. Çukurova University, PhD Thesis, 2006; 89 p., Adana.
  • Ergün M, Erkan S, Paylan İC. Cucumber mosaic virus in globe artichoke in Turkey. Canadian Journal of Plant Pathology, 2013; 35 (4), 514-517.
  • Korkmaz G, Mustafa U, Demirel S. Potato virus S (PVS)-Bitlis izolatının kılıf proteinin in silico karakterizasyonu ve konak proteini ile Docking analizi. Journal of Agriculture, 2022; 5(1), 57-67.
Year 2024, Volume: 13 Issue: 1, 1 - 6, 26.03.2024
https://doi.org/10.46810/tdfd.1266565

Abstract

References

  • Doolittle SP. A new infectious mosaic disease of cucumber. Phytopathology. 1916; 6(2):145-147.
  • Adams IP, Glover RH, Monger WA, Mumford R, Jackeviciene E, Navalinskiene M, Samuitiene M, Boonham N. Next-generation sequencing and metagenomic analysis: A universal diagnostic tool in plant virology. Molecular Plant Pathology. 2009; 10(4):537-545.
  • Edwardson JR, Christie RG. Cucumoviruses. In: Edwardson JR, Christie RG, editors. CRC handbook of viruses infecting Legumes. CRC Press: Boca Raton; 1991. p. 293-319.
  • Jacquemond M. Cucumber mosaic virus. Advances in Virus Research. 2012; 84:439-504.
  • Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RI. Cucumber mosaic virus. Advances in Virus Research. 1992; 41:281-348.
  • Roossinck MJ, Zhang L, Hellwald KH. Rearrangements in the 5' nontranslated region and phylogenetic analyses of Cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. Journal of Virology. 1999; 73(8):6752-6758.
  • Lecoq, H., and Desbiez, C. Virus of cucurbit crops in the Mediterranean Region: an ever-changing picture. In: Loebenstein G, Lecoq H, editors. Viruses and Virus Diseases of Vegetables in the Mediterranean Basin. Virus Research; 2012. p. 67-126.
  • Mijatovic M, Zdravkovic J, Markovic Z, Obradovic A. Disease intensity of some tomato viruses in Serbia. Acta Physiologiae Plantarum. 2000; 22(3):332-335.
  • Arlı-Sökmen M, Mennan H, Şevik MA, Ecevit O. Occurrence of Viruses in Field grown Pepper Crops and Some of Their Reservoir Weed Hosts in Samsun, Turkey. Phytoparasitica. 2005; 33:347-358.
  • Çağlar BK. Characterization of melon (CMV-K), tomato (CMV-D), pepper (CMV-B) isolates of cucumber mosaic virus (CMV) by biological, serological, molecular methods and effect of satellite-RNAs on virus. Adana: Çukurova University, 2006.
  • Berniak H, Kaminska M, Malinowski T. Cucumber mosaic virus groups IA and II are represented among isolates from naturally infected lilies. European Journal of Plant Pathology. 2010; 127:305-309.
  • Temfack D, Dély C, Chofong G, Mbulli A, Gentil I, Ouboum F, et al. Detection of Cucumber mosaic virus on Solanum lycopersicum L. and Capsicum annuum L. in Western region of Cameroon. Journal of Agriculture and Food Research. 2022; 8:100294.
  • Foissac X, Svanella-Dumas L, Dulucq MJ, Candresse T, Gentit P. Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). Acta Hortic. 2001; 550:37-43.
  • Günay A. The investigation of some tobacco viruses in tobacco fields of Adıyaman by multiplex RT-PCR method and the molecular characterization of some virus isolates. Van: Van Yüzüncü Yıl University; 2019.
  • Raccah B. Detection of virus diseases by advanced techniques and control. Epidemiology and control of cucurbit viruses in Israel. 1. Israeli- Turkish Workshop. Turkey: Adana; 1999. p. 45-46.
  • Kaya A, Erkan S. Detection and incidence of viruses in cucurbits grown in Izmir, Aydın, Manisa and Balıkesir provinces. Plant Protection Bulletin. 2011; 51(4):387-405.
  • Palukaitis P, Garcia-Arenal F. Cucumber mosaic virus [Internet], Description of Plant Viruses, 2003: No; 400 [cited 2023 February 20]. Available from: https://www.dpvweb.net/dpv/showdpv/?dpvno=400
  • Gümüş M, Erkan S, Tok S. Studies on determination of virus diseases in the seeds of some Cucurbitaceous species. Ege Üniversitesi Ziraat Fakültesi Dergisi. 2004; 41(1):49-56.
  • Çulal Kılıç H, Yardımcı N. Cucumber mosaic Virus in Bean Growing Areas of Çine Plain, Burdur, Turkey. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2012; 3(2):12-15.
  • Ergün M, Semih E, Paylan IC. Cucumber mosaic virus in globe artichoke in Turkey. Canadian Journal of Plant Pathology. 2013; 35(4):514-517.
  • Erkan S, Gümüş M, Paylan İC, Duman İ, Ergün M. The determination of viral agents in certain cold-season vegetables in İzmir province and its around. Ege Üniversitesi Ziraat Fakültesi Dergisi. 2013; 50(3):311-322.
  • Uzunoğulları N, Gümüş M. Detection of cucumber mosaic virus (CMV) causing natural infection on some cultured plants in Marmara Region. Trakya University Journal of Natural Sciences. 2015; 16(1):9-15.
  • Gökdağ S, Karanfil A, Korkmaz S. Çanakkale ili ıspanak alanlarındaki Şalgam mozaik virüsü ve Hıyar mozaik virüsü varlığının belirlenmesi. Bahçe. 2016; özel sayı (cilt II):166-170.
  • Güngör M, Uzunbacak H, Kutluk-Yilmaz ND, Şevik MA. Determination of viruses causing infection in spinach fields in Samsun province. Anadolu Journal of Agricultural Sciences. 2017; 32(2):164-168.
  • Katul L, Makkouk KM. Occurrence and serological relatedness of five cucurbit potyviruses in Lebanon and Syria. Phytopathologia Mediterranea. 1987; 26(1):36-42.
  • Tobias I, Szabo B, Salanki K, Sari L, Kuhlmann H, Palkovics L. Seed borne transmission of Zucchini yellow mosaic virus and Cucumber mosaic virus in Styrian Hulless group of Cucurbita pepo. Proceedings of the IX EUCARPA Meeting of Genetics and Plant Breeding of Cucurbitaceae. France: Avignon; 2008. p. 189-197.
  • Karanfil A. Prevalence and molecular characterization of Cucumber mosaic virus isolates infecting tomato plants in Marmara region of Turkey. Plant Protection Bulletin. 2021; 61(4):19-25.
  • Yardimci N, Eryigit H. Identification of Cucumber mosaic virus in tomato (Lycopersicon esculentum) growing areas in the north-west Mediterranean region of Turkey. New Zealand Journal of Crop and Horticultural Science. 2006; 34(2):173-175.
  • Güller A, Usta M. Occurrence of Cucumber mosaic cucumovirus and Watermelon mosaic potyvirus on Melon exhibiting viral symptoms in Bingöl province of Turkey and their phylogenetic affinities. Türk Tarım ve Doğa Bilimleri Dergisi. 2020;7(4):948-958.
  • Öztürk S. Survey on the virus diseases of watermelon in Diyarbakır and districts. Adana: Çukurova University; 2000.
  • Budak E. Determination and incidence of viruses in cucurbits grown in Diyarbakır and its districts. Şanlıurfa: Harran University; 2015.
  • Güneş N, Gümüş M. Detection and characterization of tomato spotted wilt virus and cucumber mosaic virus on pepper growing areas in Antalya. Journal of Agricultural Sciences. 2019;25(3):259-271.
  • Karanfil A, Korkmaz S. Detection and molecular characterization based on coat protein gene of Cucumber mosaic virus (CMV) from cowpea production fields of Çanakkale province in Turkey. Plant Protection Bulletin. 2017; 57(3):293-304.
  • Ohshima K, Matsumoto K, Yasaka R, Nishiyama M, Soejima K, Korkmaz S, et al. Temporal analysis of reassortment and molecular evolution of cucumber mosaic virus: Extra clues from its segmented genome. Virology. 2016; 487:188-197.
  • Chen S, Gu H, Wang X, Chen J, Zhu W. Multiplex RT-PCR detection of Cucumber mosaic virus subgroups and Tobamoviruses infecting Tomato using 18S rRNA as an internal control. Acta Biochim Biophys Sin. 2011; 43(6), 465-471.
  • Balsak SC, Kardeş H, Buzkan N. First report of cucumber mosaic virus in cyclamen (Cyclamen spp.) in Turkey. Journal of Plant Pathology, 2021; 103 (1), 361-362.
  • Kurtoğlu A, Korkmaz S. Determination and molecular characterization of cucumber mosaic virus (CMV) infection on spinach production fields of Çanakkale province, Turkey. The Journal of Turkish Phytopathology, 2018; 47 (2), 43-51.
  • Özdemir S, Erilmez S. First report of Alfalfa mosaic virus and Cucumber mosaic virus in pepino in Turkey. Journal of Plant Pathology, 2012; 94 (4), 91.
  • Çağlar BK. Characterization of melon (CMV-K), tomato (CMV-D), pepper (CMV-B) isolates of cucumber mosaic virus (CMV) by biological, serological, molecular methods and effect of satellite-RNAs on virus. Çukurova University, PhD Thesis, 2006; 89 p., Adana.
  • Ergün M, Erkan S, Paylan İC. Cucumber mosaic virus in globe artichoke in Turkey. Canadian Journal of Plant Pathology, 2013; 35 (4), 514-517.
  • Korkmaz G, Mustafa U, Demirel S. Potato virus S (PVS)-Bitlis izolatının kılıf proteinin in silico karakterizasyonu ve konak proteini ile Docking analizi. Journal of Agriculture, 2022; 5(1), 57-67.
There are 41 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Articles
Authors

Abdullah Güller 0000-0003-3887-4208

Serap Demirel 0000-0002-1877-0797

Mustafa Usta 0000-0002-3940-2774

Gülüstan Korkmaz 0000-0002-9032-5823

Early Pub Date March 26, 2024
Publication Date March 26, 2024
Published in Issue Year 2024 Volume: 13 Issue: 1

Cite

APA Güller, A., Demirel, S., Usta, M., Korkmaz, G. (2024). Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province. Türk Doğa Ve Fen Dergisi, 13(1), 1-6. https://doi.org/10.46810/tdfd.1266565
AMA Güller A, Demirel S, Usta M, Korkmaz G. Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province. TJNS. March 2024;13(1):1-6. doi:10.46810/tdfd.1266565
Chicago Güller, Abdullah, Serap Demirel, Mustafa Usta, and Gülüstan Korkmaz. “Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province”. Türk Doğa Ve Fen Dergisi 13, no. 1 (March 2024): 1-6. https://doi.org/10.46810/tdfd.1266565.
EndNote Güller A, Demirel S, Usta M, Korkmaz G (March 1, 2024) Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province. Türk Doğa ve Fen Dergisi 13 1 1–6.
IEEE A. Güller, S. Demirel, M. Usta, and G. Korkmaz, “Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province”, TJNS, vol. 13, no. 1, pp. 1–6, 2024, doi: 10.46810/tdfd.1266565.
ISNAD Güller, Abdullah et al. “Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province”. Türk Doğa ve Fen Dergisi 13/1 (March 2024), 1-6. https://doi.org/10.46810/tdfd.1266565.
JAMA Güller A, Demirel S, Usta M, Korkmaz G. Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province. TJNS. 2024;13:1–6.
MLA Güller, Abdullah et al. “Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province”. Türk Doğa Ve Fen Dergisi, vol. 13, no. 1, 2024, pp. 1-6, doi:10.46810/tdfd.1266565.
Vancouver Güller A, Demirel S, Usta M, Korkmaz G. Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province. TJNS. 2024;13(1):1-6.

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