Süs Balığı Satışı Yapılan Akvaryumlardan İzole Edilen Gram Negatif Bakterilerin Antimikrobiyal Direnç Profillerinin Araştırılması

Year 2017, Volume: 74 Issue: EK-1, 35 - 40, 01.12.2017

Esra Özkaya Kurtuluş Buruk Neşe Kaklıkkaya İlknur Tosun

Abstract

Amaç: Süs balığı satışı yapılan iş yerlerinde bulunan akvaryumlarda çok sayıda balık bir aradadır ve çok sayıda mikroorganizma çoğalabilmektedir. Daha da önemlisi, satış yapılırken balıklar bir miktar su ile birlikte transfer edildiğinden mikroorganizmalar farklı akvaryumlara da aktarılmaktadırlar. Süs balıklarının üretilmesi esnasında balık hastalıklarının önlenmesi ya da tedavi edilmesi amacıyla akvaryumlara çeşitli antimikrobiyaller eklenebilmektedir. Bu da antimikrobiyal direnç gelişimine neden olabilmektedir. Çalışmamızın amacı; evcil hayvan satışı yapılan mağazalardaki süs balığı akvaryumlarından alınan su örneklerinde bulunan Gram negatif bakterilerin varlığı ve antimikrobiyal duyarlılıklarının belirlenmesidir.Yöntem: Trabzon ilinde hizmet vermekte olan beş evcil hayvan satış mağazasında bulunan 27 adet süs balığı akvaryumundan su örnekleri alındı. Bu örneklerin %5 Koyun Kanlı Agar ve Eosin-MethyleneBlue Agar besiyerlerine ekimleri yapıldı. Üreme olan plaklardan alınan koloniler, MALDI-TOF MS kullanılarak tanımlandı. Antimikrobiyal duyarlılık testleri KirbyBauer disk difüzyon yöntemi ile çalışıldı.Bulgular: Beş evcil hayvan satış mağazasından alınan 27 adet süs balığı akvaryum suyu örneğinde 31 adet Gram negatif bakteri tanımlandı. En sık %25,8 ile Citrobacter Resistance to imipenem, meropenem and ertapenem, which are commonly used antibiotics in clinical treatments, was determined in one isolate among the four Aeromonas hydrophila isolates. Resistance to trimethoprim-sulfamethoxazole and ciprofloxacin was seen in members of Enterobactericeae, Pseudomonas spp. and Acinetobacter spp. isolated in this study.Conclusion: Fish have an important role in zoonotic transmission. Antimicrobial resistant strains can cause serious infections which have limited therapeutic options. As a result, it should be considered that contact with aquarium water may be one of the important factors in the spread of resistant microorganisms. In our study, it is the most extensive study that has been conducted to investigate the presence of antimicrobial resistance in Gram negative bacteria isolated from aquarium water.Sonuç: Balıkların zoonotik bulaştırmadaki rolleri bilinmektedir. Özellikle antimikrobiyal dirençli suşlar, tedavi seçeneği sınırlanmış ciddi enfeksiyonlara neden olabilmektedir. Dolayısıyla izlemi konusunda hassas davranılması gereklidir. Sonuç olarak akvaryum suları ile temasın dirençli mikroorganizmaların yayılımında önemli faktörlerden biri olabileceği göz önünde bulundurulmalıdır. Çalışmamız, akvaryum sularından izole edilen “Gram negatif bakterilerde antimikrobiyal direnç varlığı” için geniş bir çalışma olduğunu düşünmekteyiz

Keywords

akvaryum, Gram negatif bakteriler, antimikrobiyal direnç

Investigation of Antimicrobial Resistance Profiles of Gram Negative Bacteria Isolated from Ornamental Fish Aquariums in Petshops

Abstract

Objective: In aquariums in shops which sell ornamental fish, a large number of fish are present together, and many microorganisms can grow in them. Moreover, since the fish which are sold are transferred with some water the microorganisms are transported to different aquariums as well. A variety of antimicrobials can be added to aquariums to prevent or treat fish diseases during the breeding of ornamental fish, and this can lead to the development of antimicrobial resistance. The aim of this study is to determine the presence and antimicrobial susceptibility of Gram negative bacteria isolated from water samples taken from ornamental fish aquariums in stores where pets are sold.Methods: Water samples were collected from 27 ornamental fish aquariums in five pet shops serving in Trabzon. These specimens were inoculated on 5% Sheep Blood Agar and Eosin-Methylene-Blue Agar media. Colonies from plaques were identified using MALDI-TOF MS. Antimicrobial susceptibility tests were performed using the Kirby-Bauer disc diffusion method. Results: A total of 31 Gram negative bacteria were identified in the 27 samples of ornamental fish aquarium water taken from the five pet shops. Citrobacter freundii was detected most frequently with a rate of 25.8%. Resistance to imipenem, meropenem and ertapenem, which are commonly used antibiotics in clinical treatments, was determined in one isolate among the four Aeromonas hydrophila isolates. Resistance to trimethoprim-sulfamethoxazole and ciprofloxacin was seen in members of Enterobactericeae, Pseudomonas spp. and Acinetobacter spp. isolated in this study.Conclusion: Fish have an important role in zoonotic transmission. Antimicrobial resistant strains can cause serious infections which have limited therapeutic options. As a result, it should be considered that contact with aquarium water may be one of the important factors in the spread of resistant microorganisms. In our study, it is the most extensive study that has been conducted to investigate the presence of antimicrobial resistance in Gram negative bacteria isolated from aquarium water

Keywords

aquarium, Gram negative, antimicrobial resistance

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