İndirekt Hemaglütinasyon Yöntemiyle anti-Echinococcus granulosus Antikorları Pozitif Saptanan Serum Örneklerinin Western Blot Testi ile Değerlendirilmesi

Öz

Amaç: Echinococcus granulosus larvasının insanlarda sebep olduğu kistik ekinokokkoz KE , ülkemizde hayvancılığın yoğun olarak yapıldığı bölgelerde yaygın görülen ve tanı için klinisyen, radyolog ve mikrobiyologların multidisipliner yaklaşımını gerektiren bir zoonozdur. Enzim-linked immünassay ELISA ve indirekt hemaglütinasyon IHA , hastaların tanısında ilk sırada tercih edilirken Western Blot WB testi daha çok doğrulama amacıyla kullanılmaktadır. Fakat serolojik testlerin hastalığın tanı ve takibinde tek başına kullanımı, değişken duyarlılık ve özgüllük oranları nedeniyle önerilmemekte, uygun tanı için birden fazla serolojik testin kullanımı gerekmektedir. Bu çalışmada, Aralık 2015-2016 tarihleri arasında Gazi Üniversitesi Tıp Fakültesi Mikrobiyoloji Laboratuvarı’na KE şüphesiyle gönderilen hasta serumlarında IHA yöntemiyle titrasyon veren örneklerin doğrulama testi olarak kabul edilen WB yöntemi ile değerlendirilmesi ve iki test arasındaki tutarlılığın saptanması amaçlanmıştır.Yöntem: KE şüpheli örnekler, E. granulosus antijenleri ile hazırlanmış IHA Fumouze Laboratoires

Anahtar Kelimeler

• Kistik ekinokokoz, seroloji, tanı, indirekt hemaglütinasyon testi, western blot

Western blot assay of anti-Echinococcus granulosus antibody positive serum samples by indirect haemagglutination method

Abstract

Objective: Cystic echinococcosis CE is a zoonotic disease mainly caused by the larvae of Echinococcus granulosus which is common in rural areas in Turkey. A multidisciplinary approach consisting of clinicians, radiologists and microbiologists is required for the proper diagnosis of the disease. Enzyme-linked immunosorbent assay ELISA and indirect hemagglutination IHA tests are preferred in the primer diagnosis of cystic echinococcosis CE , while western blot WB is used to confirm the disease. However the use of serologic tests alone in diagnosis and follow-up of the disease is not recommended due to variable sensitivity and specificity rates and multiple serologic tests are required for appropriate diagnosis. In this study, it was aimed to compare the test results of patients sera sent to Gazi University Medical Faculty Microbiology Laboratory, between December 2015 and December 2016, with the preliminary diagnosis of CE, by WB test after those titrated with IHA. It is also aimed to determine the consistency between the two tests.Methods: CE suspicious specimens were first tested

Keywords

• Cystic echinococcosis, serology, diagnosis, indirect haemagglutination test, western blot

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