Navicula cryptocephala'da Klorpirifos Pestisitinin Birikme, Eliminasyon Miktarları ve Bazı Biyobelirteç Yanıtlarının Belirlenmesi

Abstract

Tarımsal alanlarda zararlılarla mücadele için kullanılan klorpirifos (CPF) gibi pestisitler, çeşitli yollarla sucul çevre ile karışarak sucul çevreye tehlike oluşturmaktadır. Bu çalışmada, Navicula cryptocephala'da klorpirifos kullanılarak birikim, eliminasyon miktarları ve bazı biyomarker tepkileri incelenmiştir. EC50 değerleri 0,19 mg/L olarak belirlenmiş ve akut toksisite değerleri EC50 değerlerinin 1/8, 1/4 ve 1/2'sinde gerçekleştirilmiştir. Model organizmalar 24, 48, 72, 96 ve 120 gün (eliminasyon süresi) boyunca CPF'ye maruz bırakılmıştır. 24 ve 96 saatte alınan numunelerden birikim miktarları Atomik Absorpsiyon Spektrofotometrisi (AAS) kütle spektrometrisi kullanılarak ölçülmüştür. Elde edilen süpernatantlarla, lipit peroksidasyon (TBARS) ve indirgenmiş glutatyon (GSH) seviyeleri, süperoksit dismutaz (SOD) enzim aktivitesi, glutatyon peroksidaz (GPx) enzim aktivitesi, katalaz (CAT) enzim aktivitesi ELISA mikroplaka okuyucu ile belirlendi. Araştırma verilerine göre, mikroalglerde CPF aktif maddesinin biyobirikim miktarının, uygulama konsantrasyonu ve süresi arttıkça arttığı belirlenmiştir. Biyomarker parametrelerinin, uygulama gruplarına kıyasla kontrol ve eliminasyon gruplarında istatistiksel olarak anlamlı değişiklikler gösterdiği belirlenmiştir.

Keywords

• navicula cryptocephala
• Chlorpyrifos
• birikim
• biyobelirteç

Determination of Accumulation, Elimination Amounts and Some Biomarker Responses of Chlorpyrifos Pesticide in Navicula cryptocephala

Abstract

Pesticides such as chlorpyrifos (CPF), which are used to combat pests in agricultural areas, mix with the aquatic environment in various ways and pose a danger to the aquatic environment. In this study, chlorpyrifos was used in Navicula cryptocephala it was aimed to examine the accumulation, elimination amounts and some biomarker responses. EC50 values were determined as 0.19 mg/L and acute toxicity values were realized at 1/8, 1/4 and 1/2 of EC50 values. Model organisms were exposed to CPF for 24, 48, 72, 96 and 120 days (elimination period). Accumulation amounts from the samples taken at 24 and 96 hours were measured using Atomic Absorption Spectophotometry (AAS) mass spectrometry. With the supernatants obtained, lipid peroxidation (TBARS) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GPx) enzyme activity, catalase (CAT) enzyme activity were determined with an ELISA microplate reader. According to research data, it was determined that the bioaccumulation amount of the CPF active ingredient in microalgae increased as the application concentration and duration increased. It was determined that the biomarker parameters showed statistically significant changes in the control and elimination groups compared to the application groups.

Keywords

• Navicula cryptocephala
• Cylorpyrifos
• accumilation
• biomarker

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