Year 2017, Volume 8 , Issue 2, Pages 43 - 51 2017-06-18

Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi
Effect of montelukast on hepatic and renal ischemia/reperfusion injury

Ali Baran BUDAK [1] , Kemal KORKMAZ [2] , Hikmet Selçuk GEDİK [3] , Gökhan LAFÇI [4] , Ertuğrul KAYA [5] , Handan ANKARALI [6] , Serdar GÜNAYDIN [7]


Amaç: Torakoabdominal aort anevrizması cerrahisinin sebep olabildiği splanknik iskemi, karaciğer ve/veya böbrek hasarının neden olduğu ciddi postoperatif komplikasyonlarla sonuçlanabilir. Bu çalışmada seçici tersinir bir CysLT1 reseptör antagonisti olan montelukastın, aortanın geçici oklüzyonuyla yaratılan böbrek ve karaciğer iskemi-reperfüzyon (I/R) hasarı üzerindeki koruyucu etkisini incelemek için tasarlandı.

 Gereç ve Yöntemler: Yirmi bir erkek Sprague-Dawley tipi sıçan, rasgele üç gruba (her grupta n=7) ayrıldı; G1 (aort oklüzyonu ve montelukast uygulaması yok), G2 (45 dakika aort oklüzyonu; montelukast uygulması yok) ve G3 (45 dakika aort oklüzyonu, 10 mg/kg montelukast uygulaması). Reperfüzyonun 48. saatinde böbrek ve karaciğerden alınan doku örneklerinden histopatolojik değerlendirme ve HSP 70, interlökin-6 ve miyeloperoksidaz (MPO) ile immünohistokimyasal boyama yapıldı.

 Bulgular: Böbrekten alınan doku örnekleri tübüler hücre dejenerasyonu, sitoplazmik vakuol formasyonu, hemoraji ve tübüler dilatasyon açısındna incelendi ve gruplar arasında istatistiksel olarak anlamlı fark bulundu (P = 0.001, P = 0.017, P = 0.001). G1 ve G3 gruplarında HSP-70 ile boyanmada G2 ye göre anlamlı fark saptandı (P = 0.001). G3 karaciğer örneklerinde hepatosit dejenerasyonu G1 ve G2 den anlamlı olarak düşüktü (P = 0.027).

 Sonuçlar: Yapılan çalışmanın bulguları, montelukastın böbrek ve karaciğer üzerinde koruyucu etkileri olduğunu histopatolojik ve immünohistokimyasal olarak göstermiştir. Montelukastın koruyucu etkileri, ilacın antioksidan ve antiinflamatuar etkilerine bağlanabilir. 

Aim: The thoracoabdominal aortic aneurysm surgery may cause splanchnic ischemia and may result in severe postoperative complications caused by liver and/or kidney injury. This study investigated the effect of temporary occlusion of the aorta on the development of I/R injury of liver and kidney and the protective effect of montelukast, a selective reverse CysLT1 receptor antagosit.

 Material and Methods: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n=7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). At the 48th hour of reperfusion, kidney and liver samples were dissected for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO).

 Results: Tissue samples taken from the kidney showed significant intergroup differences in terms of tubular cell degeneration, cytoplasmic vacuole formation, hemorrhage and tubular dilatation (P = 0.001, P = 0.017, P = 0.001, P = 0.001 respectively). There was a significant difference with regards to HSP-70 stain (P = 0.01) in favor of G1 and G3 with respect to G2. Hepatocyte degeneration in the liver samples of G3 was significantly lower than that of G1 and G2 (P = 0.027).

 Conclusion: The findings of the current study demonstrated that montelukast has protective effects on both kidney and liver; and were confirmed   histopathologically and immunohistochemically. The protective effects of montelukast can be attributed to its potential of anti-oxidative and anti-inflammatory actions.

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Subjects Health Care Sciences and Services
Journal Section Orıgınal Artıcle
Authors

Author: Ali Baran BUDAK

Author: Kemal KORKMAZ

Author: Hikmet Selçuk GEDİK

Author: Gökhan LAFÇI

Author: Ertuğrul KAYA

Author: Handan ANKARALI

Author: Serdar GÜNAYDIN

Dates

Publication Date : June 18, 2017

Bibtex @research article { tjcl304530, journal = {Turkish Journal of Clinics and Laboratory}, issn = {}, eissn = {2149-8296}, address = {}, publisher = {DNT Ortadoğu Yayıncılık A.Ş.}, year = {2017}, volume = {8}, pages = {43 - 51}, doi = {10.18663/tjcl.304530}, title = {Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi}, key = {cite}, author = {BUDAK, Ali Baran and KORKMAZ, Kemal and GEDİK, Hikmet Selçuk and LAFÇI, Gökhan and KAYA, Ertuğrul and ANKARALI, Handan and GÜNAYDIN, Serdar} }
APA BUDAK, A , KORKMAZ, K , GEDİK, H , LAFÇI, G , KAYA, E , ANKARALI, H , GÜNAYDIN, S . (2017). Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi. Turkish Journal of Clinics and Laboratory , 8 (2) , 43-51 . DOI: 10.18663/tjcl.304530
MLA BUDAK, A , KORKMAZ, K , GEDİK, H , LAFÇI, G , KAYA, E , ANKARALI, H , GÜNAYDIN, S . "Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi". Turkish Journal of Clinics and Laboratory 8 (2017 ): 43-51 <https://dergipark.org.tr/en/pub/tjcl/issue/29774/304530>
Chicago BUDAK, A , KORKMAZ, K , GEDİK, H , LAFÇI, G , KAYA, E , ANKARALI, H , GÜNAYDIN, S . "Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi". Turkish Journal of Clinics and Laboratory 8 (2017 ): 43-51
RIS TY - JOUR T1 - Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi AU - Ali Baran BUDAK , Kemal KORKMAZ , Hikmet Selçuk GEDİK , Gökhan LAFÇI , Ertuğrul KAYA , Handan ANKARALI , Serdar GÜNAYDIN Y1 - 2017 PY - 2017 N1 - doi: 10.18663/tjcl.304530 DO - 10.18663/tjcl.304530 T2 - Turkish Journal of Clinics and Laboratory JF - Journal JO - JOR SP - 43 EP - 51 VL - 8 IS - 2 SN - -2149-8296 M3 - doi: 10.18663/tjcl.304530 UR - https://doi.org/10.18663/tjcl.304530 Y2 - 2016 ER -
EndNote %0 Turkish Journal of Clinics and Laboratory Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi %A Ali Baran BUDAK , Kemal KORKMAZ , Hikmet Selçuk GEDİK , Gökhan LAFÇI , Ertuğrul KAYA , Handan ANKARALI , Serdar GÜNAYDIN %T Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi %D 2017 %J Turkish Journal of Clinics and Laboratory %P -2149-8296 %V 8 %N 2 %R doi: 10.18663/tjcl.304530 %U 10.18663/tjcl.304530
ISNAD BUDAK, Ali Baran , KORKMAZ, Kemal , GEDİK, Hikmet Selçuk , LAFÇI, Gökhan , KAYA, Ertuğrul , ANKARALI, Handan , GÜNAYDIN, Serdar . "Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi". Turkish Journal of Clinics and Laboratory 8 / 2 (June 2017): 43-51 . https://doi.org/10.18663/tjcl.304530
AMA BUDAK A , KORKMAZ K , GEDİK H , LAFÇI G , KAYA E , ANKARALI H , GÜNAYDIN S . Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi. TJCL. 2017; 8(2): 43-51.
Vancouver BUDAK A , KORKMAZ K , GEDİK H , LAFÇI G , KAYA E , ANKARALI H , GÜNAYDIN S . Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi. Turkish Journal of Clinics and Laboratory. 2017; 8(2): 51-43.