IDENTIFICATION OF A SCAR MARKER LINKED TO A SHATTERING RESISTANCE TRAIT IN SESAME

Year 2017, Volume: 22 Issue: 2, 258 - 265, 15.12.2017

Chalermpol Phumıchaı Weerachai Matthayatthaworn Nipha Chuenpom Arunee Wongkaew Phakaked Somsaeng Tanapong Yodyıngyong Pherawich Panklang Sujin Jenweerawat Yaowamarn Keawsaard Thitaporn Phumıchaı Tanee Sreewongchaı Rangsarid Kaveeta

https://doi.org/10.17557/tjfc.359707

Cited By: 2

Abstract

Sesame (Sesamum indicum L.) is one of the most important oil crops in temperate and tropical regions and is
grown worldwide over an area of 5179 (hg ha-1
) to produce 5.469.024 tonnes of seed. Capsule shattering before
or during harvest can cause yield losses of greater than 50%. The objectives of the present study were to
evaluate the inheritance of resistance to capsule shattering in the F2 derived from a cross between Cplus1, a
sesame line with shattering-resistant capsules (Sh1Sh1Sh2Sh2), and KUAOX25, a line with shatteringsusceptible
capsules (sh1sh1sh2sh2-
); and use bulked segregant analysis and Amplified Fragment Length
Polymorphisms (AFLPs) to identify Sequence Characterized Amplified Region (SCAR) markers associated
with shattering resistance. After screening 192 AFLP primer combinations, nine polymorphic bands were
identified, and one of these AFLPs was developed into a Si-SR-32-19 SCAR that could distinguish between
shattering-susceptible and shattering-resistant phenotypes.

Keywords

AFLP, BSA, SCAR, Sesamum indicum L., shattering resistance

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