In this study was aimed to investigate antioxidant, anticholinesterase inhibitory, tyrosinase inhibitory and urease inhibitory activities of the synthesized fifteen hydrazone compounds (1-15). According to the antioxidant activity assay results, compound 8 (IC50= 22.40±0.87 μM) and 6 (IC50= 29.48±0.71 μM) showed the best lipid peroxidation inhibitory activity. The compound 6 and 8 exhibited better activity IC50 value of 21.34±0.11 and 25.33±0.27 μM, respectively, than standard BHT (IC50= 54.97±0.99 μM) in DPPH free scavenging activity. Among the tested compound, compound 6 (IC50= 11.71±0.28 μM) and 8 (IC50= 16.45±0.31 μM) showed the best cation radical scavenging activity. Compound 6, 11, 10, 8, 1, 7, and 14 indicated the best CUPRAC capacity activity with an A0.5 value of 15.12±0.00, 17.91±0.01, 21.18±0.00, 23.25±0.03, 24.29±0.01, 26.83±0.01, and 32.35±0.02 μM, respectively, than the standard antioxidants, α-tocopherol (A0.5=40.55±0.04 μM) and BHA (A0.5=32.71±0.02 μM) using as standards. Compound 14 (IC50=11.38±0.44 μM) and 6 (IC50=18.77±0.61 μM) showed the highest acetylcholinesterase inhibitory activity. Among them, compound 2, 14, 6, 3, 13, 8, 10, 11, and 12 were determined to have butyrylcholinesterase inhibitory activity IC50 value of 24.12±0.65, 27.46±0.44, 29.33±0.21, 30.26±0.05, 35.60±0.53, 37.42±0.48, 39.44±0.74, 42.75±0.22, and 45.40±0.76 μM, respectively, than the standard galantamine (IC50=44.03±0.14 μM). According to the tyrosinase assay results, compound 3 (IC50=12.20±0.44 mM), 4 (IC50=14.76±0.90 mM), 15 (IC50=16.28±0.41 mM), 5 (IC50=18.39±0.87 mM), and 2 (IC50=20.12±0.33 mM) exhibited the best tyrosinase inhibitory activity. Compound 6 (IC50=19.20±0.48 mM) and 14 (IC50=22.62±0.38 μM) founded that the urease inhibitory activity are significant when compared with the thiourea (IC50=23.08±0.19 μM).
Hydrazone antioxidant activity tyrosinase inhibitory activity urease inhibitory activity anticholinesterase inhibitory activity
Bu çalışmada, 15 hidrazon molekülünün (1-15) antioksidan, antikolinesteraz inhibisyon, tirozinaz inhibisyon ve üreaz inhibisyon aktivitelerinin araştırılması amaçlandı. Antioksidan aktivite test sonuçlarına göre, 8 (IC50=22.40±0.87 μM) ve 6 (IC50=29.48±0.71 μM) numaralı moleküller en iyi lipit peroksidaz inhibisyon aktivitesine sahip olduğunu gösterdi. 6 ve 8 numaralı moleküllerin DPPH serbest radikal giderim aktivitesi, standart BHT’ya (IC50=54.97±0.99 μM) göre sırasıyla 21.34±0.11 ve 25.33±0.27 μM’lik IC50 değerleri ile test edilen moleküllere istinaden daha iyi aktivite sergilediği saptandı. Test edilen moleküller arasında 6 (IC50=11.71±0.28 μM) ve 8 (IC50=16.45±0.31 μM) numaralı moleküllerin en iyi katyon radikal giderim aktivitesine sahip olduğunu bulundu. 6, 11, 10, 8, 1, 7 ve 14 numaralı moleküller α-tokoferol (A0.5=40.55±0.04 μM) ve BHA (A0.5=32.71±0.02 μM) antioksidan standartlarına göre 15.12±0.00, 17.91±0.01, 21.18±0.00, 23.25±0.03, 24.29±0.01, 26.83±0.01 ve 32.35±0.02 μM’lik A0.5 değerleriyle en iyi CUPRAC kapasite aktivitesine sahip olduğu saptandı. 14 (IC50=11.38±0.44 μM) ve 6 (IC50=18.77±0.61 μM) numaralı moleküller en iyi asetilkolinesteraz inhibisyon aktivitesi sergilerken, 2, 14, 6, 3, 13, 8, 10, 11 ve 12 numaralı moleküllerin ise BChE standardı olarak kullanılan galantamine (IC50=44.03±0.14 μM) göre sırasıyla 24.12±0.65, 27.46±0.44, 29.33±0.21, 30.26±0.05, 35.60±0.53, 37.42±0.48, 39.44±0.74, 42.75±0.22, and 45.40±0.76 μM’lik IC50 değerleriyle en iyi butirilkolinesteraz inhibisyon aktivitesine sahip oldukları belirlendi. Tiroazinaz test sonuçlarına göre, 3 (IC50=12.20±0.44 mM), 4 (IC50=14.76±0.90 mM), 15 (IC50=16.28±0.41 mM), 5 (IC50=18.39±0.87 mM), ve 2 (IC50=20.12±0.33 mM) numaralı moleküllerin en iyi tirozinaz inhibisyon aktivitesi sergilendiği bulundu. Test edilen hidrazon moleküllerinin üreaz inhibisyon aktivitesi standart olarak kullanılan tiyoüre (IC50=23.08±0.19 μM) ile mukayese edilediğinde 6 (IC50=19.20±0.48 mM) ve 4 (IC50=22.62±0.38 μM) numaralı hidrazon moleküllerinin daha iyi olduğu tespit edildi.
Hidrazon antioksidan aktivite antikolinesteraz inhibisyon aktivite tirozinaz inhibisyon aktivite üreaz inhibisyon aktivite
Primary Language | English |
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Journal Section | 2017-Articles |
Authors | |
Publication Date | December 30, 2017 |
Acceptance Date | December 29, 2017 |
Published in Issue | Year 2017 Volume: 2 Issue: 2 |