Bazı Entomopatojen Fungusların Entomopatojen Nematod Steinernema feltiae ile Etkileşimlerine İlişkin Bir Ön Çalışma

Year 2024, Volume: 6 Issue: 2, 143 - 147

Şerife Evrim Arıcı , Fatma Gül Göze Özdemir

https://doi.org/10.55979/tjse.1589147

Abstract

Entomopatojen funguslar (EPF) ve entomopatojen nematodlar (EPN), çok çeşitli böcekleri enfekte edebilen ve öldürebilen toprak organizmalarıdır. Bu organizmalar böcek zararlılarının yönetiminde sıklıkla biyolojik kontrol etmenleri olarak kullanılır. Hem EPF hem de EPN’ler aynı toprak ortamında bulunabilirler ve aynı böcek konukçusu içerisinde rekabet edebilir; sinerjik etkilerden antagonistik etkiye kadar değişen sonuçlar gösterebilirler. Bu çalışma EPN Steinernema feltiae ile farklı EPF türleri arasındaki ilişkiyi belirlemek ve biyolojik kontrolde kullanımları üzerindeki olası etkilerini araştırmak için yapılmıştır. Elde edilen sonuçlara göre nematod yumurta patojeni olan Purpureocillium lilacinum uygulamalarında EPN ölümü belirlenmemiş ve reizolasyon çalışmalarında fungus elde edilememiştir. Fusarium subgulitinans Fs-8 izolatı S. feltiae’da infektif juvenil (IJs) ölümüne (3.5) en az etkili olan fungus olmuştur. Beauveria bassiana BIM-001, Metarhizium robertii, M. anisopliae ISP-12 ve B. varroe izolatları S. feltiae üzerinde ölümlere neden olmuştur. M. anisopliae ISP-1’de 15.3 IJs, B. bassiana’ BY-2 ‘de 15. 5 IJs, M. anisopliae ISP-17’de 16.3 IJs ölürken, P. lilacinum’de 0.75 IJs, F. subglutinans Fs-8’de ise 3.5 IJs ölümleri gözlenmiştir. Elde edilen sonuçlara göre, EPF’lerin S. feltiae üzerinde öldürücü etkisinin olabileceği ve bu etkinin tür bazında değişkenlik gösterdiği belirlenmiştir. Bunun nedeni EPF’ler arasındaki mekanizma, patojenite ve toksin farklılığı olabilir. Zararlıların biyolojik kontrolünde EPF ve EPN kombinasyonlarına yönelik ayrıntılı çalışmaların yapılması gerekmektedir.

Keywords

Beauveria spp, Biyolojik mücadele, Fusarium subgulitinans, Metarhizium spp., Purpureocillium lilacinum

Thanks

Purpureocillium lilacinum, Beauveria varroae ve Metarhizium robertii, funguslarının temininde yardımcı olan Kahramanmaraş Sütçü İmam Üniversitesi Ziraat Fakültesi Bitki Koruma Bölümü’nden Prof. Dr. Kubilay ER’e, Beauveria bassiana (BY-2 ve BIM-001) izolatlarının temininde yardımcı olan Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü’nden Doç.Dr. Asiye UZUN YİĞİT’e teşekkür ederiz.

A Preliminary Study on the Interactions Between Some Entomopathogenic Fungi and the Entomopathogenic Nematode Steinernema feltiae

Abstract

Entomopathogenic fungi (EPF) and entomopathogenic nematodes (EPN) are soil organisms that can infect and kill a wide variety of insects. These organisms are frequently used as biological control agents in the management of insect pests. Both EPF and EPN can be found in the same soil environment and can compete within the same insect host; they can show synergistic to antagonistic effects. This study was conducted to determine the relationship between EPN Steinernema feltiae and different EPF species and to investigate their possible effects on their use in biological control. According to the results obtained, EPN mortality was not detected in the applications of Purpureocillium lilacinum, the nematode egg pathogen, and the fungus could not be obtained in reisolation studies. Fusarium subgulitinans Fs-8 isolate was the least effective fungus in S. feltiae infective juvenile (IJs) mortality (3.5). Beauveria bassiana BIM-001, Metarhizium robertii, M. anisopliae ISP-12 and B. varroe isolates caused mortality on S. feltiae. While 15.3 IJs died in M. anisopliae ISP-1, 15.5 IJs died in B. bassiana BY-2, 16.3 IJs died in M. anisopliae ISP-17, 0.75 IJs died in P. lilacinum, and 3.5 IJs died in F. subglutinans Fs-8. According to the results obtained, it was determined that EPFs may have a lethal effect on S. feltiae and this effect varies depending on the species. This may be due to the differences in mechanism, pathogenicity, and toxin among EPFs. Detailed studies should be conducted on EPF and EPN combinations in the biological control of pests.

Keywords

Beauveria spp, Biyolojik mücadele, Fusarium subgulitinans, Metarhizium spp., Purpureocillium lilacinum

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