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Tohum Depo Proteinlerinin (Albumin, Globulin A, Glutelin) SDSPAGE Analizlerine göre Lathyrus L. Türlerinde Genetik Varyasyonun Belirlenmesi

Year 2015, Volume: 10 Issue: 1, 21 - 26, 01.03.2015

Abstract

Bu çalışma, Türkiye’deki doğal habitatlarından toplanan bazı Lathyrus (L. clymenum, L. ochrus, L. nissolia, L.
aphaca L. var. affinis, L. aphaca L. var. biflorus, L. aphaca L. var. pseudoaphaca, L. aphaca L. var. modestus)
türlerindeki tohum depo proteinlerinin alt fraksiyonlarının (albumin, globulin A, glutelin) band paternlerindeki
varyasyonu rapor etmektedir. Bu çalışmada, elektroforetik veriler, jel dökümantasyon sistemi ile görüntülendi ve
Quatity 1-D analiz yazılımı kullanılarak analiz edildi ve dendogramlar%4 tolerans ile UPGAMA (Unweighted
Pair-Group Arithmetic Mean) ile oluşturuldu. Taksonlar arasındaki farklılıklar gözlendi ve yedi tür açık bir
biçimde bantların durumundan ve koyuluğundan dolayı protein band paternlerine gore ayırt edildi. Bu çalışmada,
UPGAMA kullanılarak yapılan tohum depo proteinlerinin dendogramları göstermiştir ki çalışılan bütün yedi
takson iki küme oluşturmuştur. Mevcut çalışmalar göstermiştir ki L. aphaca’nın (Aphaca seksiyonunun üyeleri)
çalışılan alt türleri, L. ochrus, L. clymenum ve L. nissolia türlerine gore birbirlerine çok daha yakın
kümelenmişlerdir. Genel olarak bu çalışmanın sonuçları Davis (1970), Kupicha (1983) ve Doğan vd. (1992)’nin
morfolojik çalışmalarını desteklemektedir.

Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin)

Year 2015, Volume: 10 Issue: 1, 21 - 26, 01.03.2015

Abstract

The present study reports on variations for banding pattern of subfractions (albumins, globulin A, glutelins) of
seed storage proteins in some Lathyrus species (L. clymenum, L. ochrus, L. nissolia, L. aphaca L. var. affinis, L.
aphaca L. var. biflorus, L. aphaca L. var. pseudoaphaca, L. aphaca L. var. modestus) collected from their
natural habitats in Turkey. Electrophoretic data were documented by using a gel documentation system and
analysed using Quantity 1-D analysis software; dendrograms were produced with 4.0 % tolerance in UPGAMA
(Unweighted Pair-Group Arithmetic Mean). The differences between taxa were observed and all seven species
were clearly identifiable from their protein banding patterns in view of both strength and positioning of the
bands. The dendrograms of seed storage proteins using UPGAMA showed that all the studied taxa formed two
clusters in this study. The present results demonstrated that the four studied subspecies of L. aphaca (members
of section Aphaca) are much more closely related to each other rather than L. ochrus, L. clymenum and L.
nissolia. The results of the present study generally support morphological classifications of Davis (1970),
Kupicha (1983) and Dogan et al. (1992).

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Details

Primary Language English
Subjects Engineering
Other ID JA65NC93YD
Journal Section Articles
Authors

İ. Emre This is me

D. Turgut-balık This is me

H. Genç This is me

A. Şahin This is me

Publication Date March 1, 2015
Submission Date March 1, 2015
Published in Issue Year 2015 Volume: 10 Issue: 1

Cite

APA Emre, İ., Turgut-balık, D., Genç, H., Şahin, A. (2015). Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin). Turkish Journal of Science and Technology, 10(1), 21-26.
AMA Emre İ, Turgut-balık D, Genç H, Şahin A. Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin). TJST. March 2015;10(1):21-26.
Chicago Emre, İ., D. Turgut-balık, H. Genç, and A. Şahin. “Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin)”. Turkish Journal of Science and Technology 10, no. 1 (March 2015): 21-26.
EndNote Emre İ, Turgut-balık D, Genç H, Şahin A (March 1, 2015) Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin). Turkish Journal of Science and Technology 10 1 21–26.
IEEE İ. Emre, D. Turgut-balık, H. Genç, and A. Şahin, “Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin)”, TJST, vol. 10, no. 1, pp. 21–26, 2015.
ISNAD Emre, İ. et al. “Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin)”. Turkish Journal of Science and Technology 10/1 (March 2015), 21-26.
JAMA Emre İ, Turgut-balık D, Genç H, Şahin A. Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin). TJST. 2015;10:21–26.
MLA Emre, İ. et al. “Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin)”. Turkish Journal of Science and Technology, vol. 10, no. 1, 2015, pp. 21-26.
Vancouver Emre İ, Turgut-balık D, Genç H, Şahin A. Determination of Genetic Variations in Lathyrus L. Species Based on SDS-PAGE Analyses of Seed Storage Proteins (Albumin, Globulin A, Glutelin). TJST. 2015;10(1):21-6.