Effect of some vitamins on antioxidant/prooxidant parameters in Sodium Fluoride (NaF)-treated Cell Line (hFOB 1.19)

Year 2017, Volume: 1 Issue: 1, 1 - 6, 01.12.2017

Veysel Yuksek , Sedat Cetın , Ayse Usta , Ahmet Ufuk Komuroglu , Semiha Dede

Abstract

Objective: This study was planned to determine the effect of certain
vitamin applications on antioxidant and oxidant parameters in the osteoblast
cell line exposed to sodium fluoride in
vitro and to evaluate the protective role of certain vitamins against
possible toxic effects of fluoride.

Materials
and Methods: Cells were replicated in vitro conditions with regular
passaging 2-3 times weekly. MTF viability test was used to determine IC₅₀ of
NaF (5000μM) and proliferative doses of vitamins (Vitamin A: 10μM, Vitamin D:
10μM, Vitamin E: 60μM, Vitamin C: 100μM) for hFOB 1.19 cells. Cells were sown
in flasks as so to be 10⁶. The study groups were identified as
control, NaF, vitamins and NaF+vitamins. After incubation for 24 hours, cells
treated with trypsin were prepared by freeze/thaw method and MTT viability
test, TAS, SOD, GSH, CAT, TOS and MDA analyzes were performed on these samples.

Results: In the hFOB 1.19 cell line, TAS levels decreased
significantly in the NaF group (p≤0.05), but were close to the control group in
NaF+vitamin groups with the exception of vitamin C. However, there was no
difference between the groups in terms of GSH level and CAT and SOD activities
when the control and NaF groups were compared. It was observed that TOS level
increased significantly in the NaF group (p<0.05), decreased in the
NaF+vitamin groups and were lower in the NaF+vitamin C and E groups than the
control group (p <0.05). While OSI was the highest in the NaF group, no
significant difference in MDA level was observed compared with the control
group.

Conclusion: As a result, it was found that NaF administration in the
osteoblast cell line increased oxidative stress and decreased following vitamin
application. It was found that the effect of NaF administration in the
osteoblast cell line on cell viability was consistent with the oxidative
stability and that the vitamin application conformably changed cell viability
and oxidative balance.

Keywords

NaF, cell culture, antioxidant, oxidative state

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