Research Article
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Year 2022, Volume 6, Issue 1, 1 - 8, 29.03.2022
https://doi.org/10.47748/tjvr.993547

Abstract

References

  • Altinok I, Balta F, Capkin E, Kayis S. Disease of rainbow trout caused by Pseudomonas luteola. Aquaculture. 2007; 273(4): 393-397.
  • Ashraf A, Hanan AF, Fatma I, Abdelhalim A. Detection of species-specific gene thermonuclease (Nuc) in Staphylococcus aureus from chickens by Real-Time PCR assay. BVMJ. 2014; 27(2): 415‐422.
  • Bilgehan H. Clinical Microbiological Diagnosis, Methods of Examining Microbiological Morphology. Barış Publications, 2002.
  • Elhassan MM, Ozbak HA, Hemeg HA, Elmekki MA, Ahmed LM. Absence of the MecA gene in methicillin resistant Staphylococcus aureus isolated from different clinical specimens in shendi city, Sudan. Biomed Res Int, 2015; 1(1): 1-5.
  • Hamidi RM, Hosseinzadeh S, Shekarforoush SS, Poormontaseri M, Derakhshandeh A. Association between the enterotoxin production and presence of Coa, Nuc genes among Staphylococcus aureus isolated from various sources, in Shiraz. Iran J Vet Res, 2015; 16(4): 381.
  • Hoegh SV, Skov MN, Boye K, Worning P, Jensen TG, Kemp M. Variations in the Staphylococcus aureus-specific Nuc gene can potentially lead to misidentification of meticillin-susceptible and-resistant S. aureus. J Med Microbiol, 2014; 63(7): 1020-1022.
  • Kaya E, Kırmalı O, Doğan Ö, Berk D, Kaya D. The susceptibility of ampicillin and ampicillin-sulbactam to bacteria with chronic otitis media. Türk Mikrobiyol Cem Derg, 2003; 33(1): 115-117.
  • Levi K, Towner K. Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA Detection Kit. J Clin Microbiol, 2003; 41(8): 3890-3892.
  • Levinson W. Medical Microbiology & Immunology, Özgünen, T., Trans. Ankara: Güneş Bookstore, 2002.
  • Nasution GS, Suryanto D, Kusumawati RL. Detection of meca gene from methicillin resistant Staphylococcus aureus isolates of north sumatera. InIOP Conference Series: Environ. Earth Sci, 2018; 130(1): 1-7.
  • Nia KM, Sepehri G, Khatmi H, Shakibaie MR. Isolation and antimicrobial susceptibility of bacteria from chronic suppurative otitis media patients in Kerman, Iran. Iran Red Crescent Med J, 2011; 13(12): 891-894.
  • Önalan Ş. Expression differences of stress and immunity genes in rainbow trout (Oncorhynchus mykiss, Walbaum 1792) with different bacterial fish diseases. Sr J Aquacult-Bamid, 2019; 1537(1): 1-10.
  • Önalan Ş, Yavuz Hİ. Spectrophotometric determination of development differences of Lactococcus garvieae isolates in liquid media containing nanoparticles. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 2019; 1(1): 6-15.
  • Özen NS, Dağlar D, Baysan BÖ, Yıldırım Ç, Yazısız H, Öğünç D, et al. Evaluation of MRSA id chromogenic media in detecting methicillin resistant Staphylococcus aureus strains. Ankem Derg, 2011; 25(1): 31-34.
  • Seidel C, Peters S, Eschbach E, Feßler AT, Oberheitmann B, Schwarz S. Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes MecA and MecC. Vet Microbiol, 2017; 200(1): 101-106.
  • Tok D, Coşkun Ö. Antibiotic susceptibilities of microorganisms growing in the cultures of patients with chronic otitis media. TAF Prev Med Bull, 2010; 9(1): 51-54.
  • Uçan N. Determination of coagulase negative staphylococci and goat antibiotic resistance in goats with subclinical mastitis. (Master Thesis). Aydın: Adnan Menderes University, Institute of Health Sciences, 2014.
  • Willke A, Sayan M, Meriç M, Mutlu M. Early diagnosis of methicillin resistance in Real-Time PCR in Staphylococci growing in blood cultures. Mikrobiyol Bul, 2012; 46(4): 671-675.
  • Yadav R, Kumar A, Singh V, Singh J, Yadav SK. Molecular determination of methicillin resistance MecA and virulence Coa genes in Staphylococcus aureus from pyogenic clinical cases of companion animals in India. Turk J Vet Anim Sci, 2018; 42(5): 371-375.

Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR

Year 2022, Volume 6, Issue 1, 1 - 8, 29.03.2022
https://doi.org/10.47748/tjvr.993547

Abstract

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that is a member of the Firmicutes. It is often positive for catalase and nitrate reduction. Pathogenic strains often promote infections by producing virulence factors such as potent protein toxins, and the expression of a cell-surface protein that binds and inactivates antibodies. Sepsis-related to methicillin-resistant S. aureus (MRSA) has significant morbidity and high mortality rates (15-30%). The methicillin resistance for S. aureus is coded with the mecA gene, while the methicillin sensitivity is coded with the nuc gene, and they are chromosomal. Similarly, it is coded with the coagulase gene for S. aureus (Coa). In this study, the 16S rRNA gene identification by Real-Time PCR was investigated in forty S. aureus isolates, which were cultured at different times in terms of MIC and SIR tests. The isolates used in the study were determined at the gene level in terms of their differences in methicillin resistance gene (MecA), methicillin susceptibility gene (Nuc), coagulase gene (Coa) and in terms of intra-species differences were examined. As a result of the study, Staphylococcus spp. yielded positive results with 16S rRNA gene-specific primers in all isolates. Real-Time PCR analysis of the isolates with SYBRGreen-based PCR analysis was performed with 16S rRNA gene-specific primers, and the samples were confirmed to be Staphylococcus. Analysis at the family level was followed by Coa, Nuc, and MecA gene Real-Time PCR results, and it was found that, in terms of Coa and Nuc genes, 19 isolates were positive and 21 isolates were negative. In terms of MecA gene, 16 isolates were positive according to the positive sigmoidal curves and to the single peak melting values, whereas 24 isolates were found to be negative. It was thought that this study will benefit the community by contributing to the rapid and effective diagnosis and treatment of infections caused by coagulase positive and negative Staphylococci.

References

  • Altinok I, Balta F, Capkin E, Kayis S. Disease of rainbow trout caused by Pseudomonas luteola. Aquaculture. 2007; 273(4): 393-397.
  • Ashraf A, Hanan AF, Fatma I, Abdelhalim A. Detection of species-specific gene thermonuclease (Nuc) in Staphylococcus aureus from chickens by Real-Time PCR assay. BVMJ. 2014; 27(2): 415‐422.
  • Bilgehan H. Clinical Microbiological Diagnosis, Methods of Examining Microbiological Morphology. Barış Publications, 2002.
  • Elhassan MM, Ozbak HA, Hemeg HA, Elmekki MA, Ahmed LM. Absence of the MecA gene in methicillin resistant Staphylococcus aureus isolated from different clinical specimens in shendi city, Sudan. Biomed Res Int, 2015; 1(1): 1-5.
  • Hamidi RM, Hosseinzadeh S, Shekarforoush SS, Poormontaseri M, Derakhshandeh A. Association between the enterotoxin production and presence of Coa, Nuc genes among Staphylococcus aureus isolated from various sources, in Shiraz. Iran J Vet Res, 2015; 16(4): 381.
  • Hoegh SV, Skov MN, Boye K, Worning P, Jensen TG, Kemp M. Variations in the Staphylococcus aureus-specific Nuc gene can potentially lead to misidentification of meticillin-susceptible and-resistant S. aureus. J Med Microbiol, 2014; 63(7): 1020-1022.
  • Kaya E, Kırmalı O, Doğan Ö, Berk D, Kaya D. The susceptibility of ampicillin and ampicillin-sulbactam to bacteria with chronic otitis media. Türk Mikrobiyol Cem Derg, 2003; 33(1): 115-117.
  • Levi K, Towner K. Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) in blood with the EVIGENE MRSA Detection Kit. J Clin Microbiol, 2003; 41(8): 3890-3892.
  • Levinson W. Medical Microbiology & Immunology, Özgünen, T., Trans. Ankara: Güneş Bookstore, 2002.
  • Nasution GS, Suryanto D, Kusumawati RL. Detection of meca gene from methicillin resistant Staphylococcus aureus isolates of north sumatera. InIOP Conference Series: Environ. Earth Sci, 2018; 130(1): 1-7.
  • Nia KM, Sepehri G, Khatmi H, Shakibaie MR. Isolation and antimicrobial susceptibility of bacteria from chronic suppurative otitis media patients in Kerman, Iran. Iran Red Crescent Med J, 2011; 13(12): 891-894.
  • Önalan Ş. Expression differences of stress and immunity genes in rainbow trout (Oncorhynchus mykiss, Walbaum 1792) with different bacterial fish diseases. Sr J Aquacult-Bamid, 2019; 1537(1): 1-10.
  • Önalan Ş, Yavuz Hİ. Spectrophotometric determination of development differences of Lactococcus garvieae isolates in liquid media containing nanoparticles. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, 2019; 1(1): 6-15.
  • Özen NS, Dağlar D, Baysan BÖ, Yıldırım Ç, Yazısız H, Öğünç D, et al. Evaluation of MRSA id chromogenic media in detecting methicillin resistant Staphylococcus aureus strains. Ankem Derg, 2011; 25(1): 31-34.
  • Seidel C, Peters S, Eschbach E, Feßler AT, Oberheitmann B, Schwarz S. Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes MecA and MecC. Vet Microbiol, 2017; 200(1): 101-106.
  • Tok D, Coşkun Ö. Antibiotic susceptibilities of microorganisms growing in the cultures of patients with chronic otitis media. TAF Prev Med Bull, 2010; 9(1): 51-54.
  • Uçan N. Determination of coagulase negative staphylococci and goat antibiotic resistance in goats with subclinical mastitis. (Master Thesis). Aydın: Adnan Menderes University, Institute of Health Sciences, 2014.
  • Willke A, Sayan M, Meriç M, Mutlu M. Early diagnosis of methicillin resistance in Real-Time PCR in Staphylococci growing in blood cultures. Mikrobiyol Bul, 2012; 46(4): 671-675.
  • Yadav R, Kumar A, Singh V, Singh J, Yadav SK. Molecular determination of methicillin resistance MecA and virulence Coa genes in Staphylococcus aureus from pyogenic clinical cases of companion animals in India. Turk J Vet Anim Sci, 2018; 42(5): 371-375.

Details

Primary Language English
Subjects Veterinary
Journal Section 2022 Volume 6 Number 1
Authors

Abdulbaki AKSAKAL>
ADIYAMAN ÜNİVERSİTESİ
0000-0002-1622-5111
Türkiye


Şükrü ÖNALAN> (Primary Author)
VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, SU ÜRÜNLERİ FAKÜLTESİ
0000-0003-0058-5232
Türkiye


Şeyda Şilan OKALİN>
DOKUZ EYLÜL ÜNİVERSİTESİ
0000-0002-4249-8113
Türkiye

Early Pub Date March 26, 2022
Publication Date March 29, 2022
Application Date September 10, 2021
Acceptance Date December 30, 2021
Published in Issue Year 2022, Volume 6, Issue 1

Cite

Bibtex @research article { tjvr993547, journal = {Turkish Journal of Veterinary Research}, eissn = {2602-3695}, address = {}, publisher = {Ebubekir CEYLAN}, year = {2022}, volume = {6}, number = {1}, pages = {1 - 8}, doi = {10.47748/tjvr.993547}, title = {Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR}, key = {cite}, author = {Aksakal, Abdulbaki and Önalan, Şükrü and Okalin, Şeyda Şilan} }
APA Aksakal, A. , Önalan, Ş. & Okalin, Ş. Ş. (2022). Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR . Turkish Journal of Veterinary Research , 6 (1) , 1-8 . DOI: 10.47748/tjvr.993547
MLA Aksakal, A. , Önalan, Ş. , Okalin, Ş. Ş. "Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR" . Turkish Journal of Veterinary Research 6 (2022 ): 1-8 <https://dergipark.org.tr/en/pub/tjvr/issue/69094/993547>
Chicago Aksakal, A. , Önalan, Ş. , Okalin, Ş. Ş. "Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR". Turkish Journal of Veterinary Research 6 (2022 ): 1-8
RIS TY - JOUR T1 - Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR AU - AbdulbakiAksakal, ŞükrüÖnalan, Şeyda ŞilanOkalin Y1 - 2022 PY - 2022 N1 - doi: 10.47748/tjvr.993547 DO - 10.47748/tjvr.993547 T2 - Turkish Journal of Veterinary Research JF - Journal JO - JOR SP - 1 EP - 8 VL - 6 IS - 1 SN - -2602-3695 M3 - doi: 10.47748/tjvr.993547 UR - https://doi.org/10.47748/tjvr.993547 Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary Research Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR %A Abdulbaki Aksakal , Şükrü Önalan , Şeyda Şilan Okalin %T Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR %D 2022 %J Turkish Journal of Veterinary Research %P -2602-3695 %V 6 %N 1 %R doi: 10.47748/tjvr.993547 %U 10.47748/tjvr.993547
ISNAD Aksakal, Abdulbaki , Önalan, Şükrü , Okalin, Şeyda Şilan . "Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR". Turkish Journal of Veterinary Research 6 / 1 (March 2022): 1-8 . https://doi.org/10.47748/tjvr.993547
AMA Aksakal A. , Önalan Ş. , Okalin Ş. Ş. Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR. TJVR. 2022; 6(1): 1-8.
Vancouver Aksakal A. , Önalan Ş. , Okalin Ş. Ş. Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR. Turkish Journal of Veterinary Research. 2022; 6(1): 1-8.
IEEE A. Aksakal , Ş. Önalan and Ş. Ş. Okalin , "Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR", Turkish Journal of Veterinary Research, vol. 6, no. 1, pp. 1-8, Mar. 2022, doi:10.47748/tjvr.993547