The Effect of Antioxidant Proteins due to Salt Stress and Wounding in Vicia Faba against Bean Yellow Mosaic Virus

Year 2013, Volume: 3 Issue: 4, 138 - 149, 23.07.2016

Zenab Aly Torky

Abstract

Environmental stresses like salinity and wounding are very harmful to plants and cause major economical losses especially if the plant is a major crop like bean. Exposure of plants to those types of stresses cause the production of reactive oxygen species which in turn damage the plant cellular system. To reverse this lethal effect, plants have developed a counter attack mechanism to adjust the oxygen level in the cells through anti-oxidant enzymes, a process known as oxygen scavenging. In this research, experiments have been conducted to investigate the relation between the type and magnitude of the stress, together with the timeline of bean yellow mosaic virus (BYMV) inoculation and the level of anti-oxidant enzymes if any, and the resistance to the virus infection. Salinity and mechanical wounding were used on the bean plants as types of the stress, three types of salinity concentrations and two types of mechanical wounding were used, and then the leaves of the stressed plants were inoculated with the virus immediately, after 6 hours, 1 and 3 days to study the systemic effect of the stress on signaling any antioxidant enzymes on those time intervals, and in one and two weeks as well to determine the state of the enzyme in the plant. The enzymes assayed were catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), and guaiacol-specific peroxidase (POX). Results revealed that there is a correlation between the stress and the level of the enzymes in the plant. These enzymes seem to trigger the induced resistance in the bean plants to the BYMV

Keywords

Abiotic stress, antioxidant, ROS, oxygen scavenging, antiviral

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