trakya univ j nat sci

Trakya University Journal of Natural Sciences

2528-9691

Trakya University

10.23902/trkjnat.1059974

Structural Biology

Yapısal Biyoloji

SCREENING OF ACTINOMYCETES FROM Cystoseira barbata (Stackhouse) C. Agardh COMPOST FOR THEIR ENZYME AND ANTIBACTERIAL ACTIVITIES

https://orcid.org/0000-0003-2066-1546

Topatan

Zeynep Şule

GİRESUN ÜNİVERSİTESİ

https://orcid.org/0000-0002-2053-3168

Katı

Hatice

GİRESUN ÜNİVERSİTESİ

10 15 2022

23 2 113 124 01 19 2022 04 26 2022

2000

Trakya University Journal of Natural Sciences

Bacterial secondary metabolites play an essential role in biotechnological and biomedical applications. Actinomycetes are important bacterial sources of antibiotics and enzymes. Most of the antimicrobials known today have been isolated from actinomycetes, especially from the genus Streptomyces. In this study, actinomycete isolation was performed from Cystoseira barbata (Stackhouse) C. Agardh compost, collected from the Black Sea coast, by serial dilution method. A total of 73 actinomycetes isolates (BSC) were obtained from the compost samples. The ability of the isolates to produce different extracellular enzymes was investigated qualitatively. It was determined that 68.5% of the isolates have amylase, 100% cellulase, 47.9% chitinase, 94.5% pectinase, 98.6% protease and 96.3% lipase/esterase activity. Antibacterial activities of the isolates were investigated primarily using cross-streak method. Isolates showed high antibacterial activities, with 98.6 and 84.9 % against Staphylococcus aureus Rosenbach and Enterococcus faecalis (Andrewes & Horder) Schleifer & Kilpper-Bäl, respectively. Three out of six isolates (BSC-13, BSC-17, BSC-37, BSC-38, BSC-45, BSC-49) with high antibacterial activity, were screened secondarily for their antibacterial activities using double-layer method. At day 7, BSC-37 isolate showed a high inhibition (57 mm) against S. aureus. Furthermore, these six isolates were identified according to their morphological and physiological characteristics and 16S rDNA sequence analysis. 16S rDNA sequence analysis showed that the isolates with high antibacterial activity belong to Streptomyces genus. Results indicated that these isolates have great potential and may serve as a good source for the studies on bioactive natural products.

Bakteriyel sekonder metabolitler, tıbbi ve biyoteknolojik uygulamalarda önemli bir rol oynamaktadır. Aktinomisetler önemli bakteriyel antibiyotik ve enzim kaynaklarıdır. Bugün bilinen antimikrobiyallerin çoğu, aktinomisetlerden özellikle Streptomyces cinsinden izole edilmiştir. Bu çalışmada, Karadeniz kıyılarından toplanan Cystoseira barbata (Stackhouse) C. Agardh kompostundan seri seyreltme yöntemi ile aktinomiset izolasyonu yapılmıştır. Kompost örneklerinden toplam 73 aktinomiset izolatı (BSC) elde edilmiştir. İzolatların farklı hücre dışı enzimler üretme yetenekleri kalitatif olarak araştırıldı. İzolatların %68,5'inin amilaz, %100’ünün selülaz, %47,9’unun kitinaz, %94,5’inin pektinaz, %98,6’sının proteaz ve %96,3’ünün lipaz/esteraz aktivitesine sahip olduğu belirlendi. İzolatların antibakteriyel aktiviteleri öncelikle çapraz çizgi yöntemi kullanılarak araştırıldı. İzolatlar, Staphylococcus aureus Rosenbach ve Enterococcus faecalis (Andrewes & Horder) Schleifer & Kilpper-Bäl’e karşı sırasıyla %98,6 ve %84,9 ile oldukça yüksek antibakteriyel aktivite göstermektedir. Yüksek antibakteriyel aktiviteli altı izolattan (BSC-13, BSC-17, BSC-37, BSC-38, BSC-45, BSC-49) üçü, çift tabakalı yöntemle antibakteriyel aktiviteleri açısından sekonder olarak taranmıştır. 7. günde, BSC-37 izolatı, S. aureus'a karşı yüksek bir inhibisyon (57 mm) gösterdi. Ayrıca bu altı izolat, morfolojik ve fizyolojik özelliklerine ve 16S rDNA dizi analizine göre tanımlandı. 16S rDNA dizi analizi yüksek antibakteriyel aktiviteye sahip izolatların Streptomyces cinsine ait olduğunu göstermiştir. Sonuçlar, bu izolatların büyük potansiyele sahip olduğunu ve biyoaktif doğal ürünler üzerine yapılan çalışmalar için iyi bir kaynak olarak hizmet edebileceğini göstermiştir.

Brown seaweed Marine actinomycetes Antibacterial Microbial enzymes

GRU-BAP

FEN-BAP-A-140316-52

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