trakya univ j nat sci

Trakya University Journal of Natural Sciences

2528-9691

Trakya University

10.23902/trkjnat.571255

Structural Biology

Yapısal Biyoloji

OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS

https://orcid.org/0000-0002-1690-4536

Şen

Ayşe

İSTANBUL ÜNİVERSİTESİ, FEN FAKÜLTESİ

https://orcid.org/0000-0002-1690-4536

Sarsu

Fatma

Plant Breeding and Genetics Section, Joint FAO/IAEA Division

10 15 2019

20 2 129 134 05 28 2019 10 07 2019

2000

Trakya University Journal of Natural Sciences

Bitkimutantları, bitki ıslahı ve fonksiyonel gen çalışmaları için önemli biyo-kaynaklardır.Mevcut çalışmada, tuza toleranslı aday mutant buğday hatları elde etmek içinsomatik embriyogenez ile konvansiyonel kimyasal mutajenez tekniğibirleştirildi.Bu amaçla; ekmeklik buğdayda (Triticum aestivum L. cv. Adana 99) genetik varyasyonlar yaratmakiçin embriyonik kalluslara 0-5 mM Sodyum Azid (NaN3), 30 dakikaboyunca in vitro ortamda uygulandı.İşlem görmüş ve görmemiş kalluslar, somatik embriyo teşvik ortamına konularaksomatik embriyo teşviki için 3 ve 4 mM NaN3 uygulaması optimummutasyon dozları olarak tespit edildi. Ardından bu mutasyon dozları ile muameleedilen kalluslardan elde edilen somatik embriyolar tuz stresine toleransgeliştirmek için kullanılacak 125 mM NaCl içeren rejenerasyon ortamlarındatolerans açısından tarandı. NaN3 muamelesinde, orta düzeyde tuzatoleransı olan 14 mutant elde edildi. Elde edilen sonuçlar; kimyasal mutagenezile kombine halde in vitro teknikuygulamasının, popülasyonlarda yeterli genetik varyasyon oluşturmak ve 1.5yıldan daha az bir sürede dördüncü jenerasyon tuz toleranslı aday buğday mutanthatlarını ıslah sürecini hızlandırarak elde etmek için kullanışlı bir yöntemolabileceğini göstermektedir.

Plant mutants areimportant bio-resources for crop breeding and functional gene studies. In thepresent study, conventional chemical mutagenesis technique was combined withsomatic embryogenesis to obtain candidate salt tolerant mutant wheat lines. Forthis purpose, 0-5 mM Sodium Azide (NaN3) was applied for 30 minutesto embryonic calli under in vitroconditions to produce genetic variations in the bread wheat (Triticum aestivum L. cv. Adana 99).Treated and non-treated calli were put in somatic embryo induction media, and 3and 4 mM NaN3 were determined as optimum mutation doses for somaticembryo induction. The obtained somatic embryos from these optimum mutagen doses were thenscreened for tolerance in regeneration media containing 125 mM NaCl to be usedto improve tolerance to salt stress. In NaN3 treatment, 14 mutantswith moderate salt tolerance were obtained. The results suggest that the in vitro technique in combination withchemical mutagenesis may be a useful approach for accelerating breedingstrategies to create enough genetic variation in populations and to get fourthgeneration putative salt tolerant wheat mutant lines it less than 1.5 years.

Sodium azide in vitro mutagenesis salt tolerance somatic embryogenesis wheat

The Research Fund of Istanbul University

BEK-2017-24939, 24750, 45260 and 49463

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