ZEBRA BALIĞI Danio rerio (Hamilton-Buchanan) YÜZME HAREKETİNİN ÜÇ BOYUTLU DÜZLEMDE TAKİBİ

Year 2016, Volume: 17 Issue: 1, 71 - 76, 25.05.2016

Utku Güner

Abstract

Hayvan davranışlarının üç boyutlu olarak izlenmesi son yıllarda özellikle fare, sıçan ve zebra balığı gibi birçok deney hayvanında kullanılmaktadır. Temel olarak, iki düzlemde elde edilen verilerin analizi ve birleştirilmesiyle üç boyutlu hareketin oluşturulması ve analizi yapılabilir. Bu yolla elde edilecek üç boyutlu hareket tek düzlemdeki harekete göre daha doğru analiz edilebilir. Üç boyutlu hareketin belirlenmesi, özellikle balıklarda doğal hareketlerin (XYZ düzlemlerinde) daha doğru ve gerçek ortama uygun analiz edilmesini sağlar. Hayvan davranış ve aktivitelerinin izlenmesinde sayısal video izleme sistemleri manuel izlemeye göre daha avantajlıdır. Sayısal video izleme kullanıcıya bağlı hataları ortadan kaldırır ve daha hızlı ve doğru sonuç alma imkânı sağlar. Bu çalışma zebra balıkları üzerinde davranışsal aktivite izlenmesinde donanım ve yazılım sorunlarının nasıl çözülebileceğini ve zebra balığından üç boyutlu (3D) verilerin nasıl elde edilebileceğini göstermektedir.

Keywords

Zebra balığı, video izleme teknolojisi, 3D video analizi

Monitoring of Zebra Fish Danio rerio (Hamilton-Buchanan) Swimming Movement in Three Dimensions

Abstract

Three-dimensional animal behavioral monitoring has recently been used in many experimental animals such as
mouse, rat and zebra fish. In principle, it is possible to obtain and analyze a three-dimensional movement pattern by analyzing
and combining the data in the two axes. Three dimensional movement obtained by this way might be analyzed more accurately
than one dimensional movement. Determination of three dimensional movement (in XYZ axes) provides a more accurate and
natural like analysis of movements particularly in fishes. Digital video monitoring systems used in animal behavior and activity
studies are more advantageous than manual tracking. Digital video surveillance eliminates user based errors and provides to
obtain fast and more accurate results. This research shows how to solve hardware and software problems in behavioral monitoring
activity of zebra fish and how to obtain the three-dimensional (3D) data from zebra fish movements. 

Keywords

Zebrafish, video surveillance technology, 3D video analysis

References

• Atchison, G.J., Henry, M.G. & Sandheinrich, M.B. 1987. Effects of metals on fish behavior: a review. Environmental Biology of fishes, 18(1): 11-25.
• Bailey, J., Oliveri, A. & Levin, E.D. 2013. Zebrafish model systems for developmental neurobehavioral toxicology. Birth Defects Research Part C, 99(1): 14-23.
• Cachat, J., Stewart, A., Utterback, E., Hart, P., Gaikwad, S., Wong, K., Kyzar, E., Wu, N. & Kalueff, A.V. 2011a. Three-dimensional neurophenotyping of adult zebrafish behavior. PLoS ONE, 6(3): e17597.
• Cachat, J.M., Stewart, A., Utterback, E., Kyzar, E., Hart, P.C., Carlos, D., Gaikwad, S., Hook, M., Rhymes, K. & Kalueff, A.V. 2011b. Deconstructing adult zebrafish behavior with swim trace visualizations. Zebrafish neurobehavioral protocols, 191-201.
• Chejiin, J.E. & Heredia, H. 1980. Design and Test of a Tank for Behavioral-Studies in Fish. Acta Cientifica Venezolana, 31(3): 275-280.
• Fontaine, E., Lentink, D., Kranenbarg, S., Muller, U.K., van Leeuwen, J.L., Barr, A.H. & Burdick, J.W. 2008. Automated visual tracking for studying the ontogeny of zebrafish swimming. Journal of Experimental Biology, 211(8): 1305-1316.
• Gerhardt, A. 2007. Aquatic behavioral ecotoxicology—prospects and limitations. Human and Ecological Risk Assessment, 13(3): 481-491.
• Gerlai, R. 2003. Zebra fish: an uncharted behavior genetic model. Behavior genetics, 33(5): 461-468.
• Green, J., Collins, C,. Kyzar, E.J., Pham, M., Roth, A., Gaikwad, S., Cachat, J., Stewart, A.M., Landsman, S. & Grieco, F. 2012. Automated high-throughput neurophenotyping of zebrafish social behavior. Journal of neuroscience methods, 210(2): 266-271.
• Güner, U. 2013. Behavioral Changes Induced by Neonicotinoid insecticide Acetamiprid in freshwater shrimp (Caridina nilotica). Anadolu Doğa Bilimleri Dergisi, 1: 6-10.
• Mathur, P. & Guo, S. 2010. Use of zebrafish as a model to understand mechanisms of addiction and complex neurobehavioral phenotypes. Neurobiology of Disease, 40(1): 66-72.
• Medishetti, R., Sripuram, V., Yellanki, S., Rao, P., Chatti, K., Oruganti, S. & Kulkarni, P. 2013. Zebra Fish Pharmacokinetic Studies. Indian Journal of Pharmacology, 45: 251-251.
• Noldus, LP., Spink, A.J. & Tegelenbosch, R.A. 2001. EthoVision: a versatile video tracking system for automation of behavioral experiments. Behavior Research Methods, Instruments & Computers, 33(3): 398-414.
• Saverino, C. & Gerlai, R. 2008. The social zebrafish: behavioral responses to conspecific, heterospecific, and computer animated fish. Behavioural brain research, 191(1): 77-87.
• Scalzo, F.M. & Levin, E.D. 2004. The use of zebrafish (Danio rerio) as a model system in neurobehavioral toxicology. Neurotoxicology and Teratology, 26(6): 707-708.
• Scheerbaum, D. & Noack U. 2003. Sensitivity of the zebra fish embryo test to industrial effluents compared to the golden orfe toxicity test. Fresenius Environmental Bulletin, 12(6): 663-664.
• Spitzen, J., Spoor, C., Kranenbarg, S., Beeuwkes, J., Grieco, F., Noldus, L., van Leeuwen, J. & Takken, W. 2008. Track3D: Visualization and flight track analysis of Anopheles gambiae ss mosquitoes. Proceedings of Measuring Behavior, 2008: 133-135
• Srivastava, A., Denschlag, H.O., Kelber, O. & Urich K. 1990. Accumulation and Discharge Behavior of Cs-137 by Zebra Fish (Branchydanio rerio) in Different Aquatic Environments. Journal of Radioanalytical and Nuclear Chemistry-Articles, 138(1): 165-170.
• Stewart, A., Cachat, J., Wong, K., Gaikwad, S., Gilder, T., DiLeo, J., Chang, K., Utterback, E. & Kalueff, A.V. 2010. Homebase behavior of zebrafish in novelty-based paradigms. Behavioural processes, 85(2): 198-203.
• Şişman, T., İncekara, Ü. & Yıldız, Y.Ş. 2008. Determination of acute and early life stage toxicity of fat‐plant effluent using zebrafish (Danio rerio). Environmental toxicology, 23(4): 480-486.
• Vutukuru, S.S. & Basani, K. 2013. Acute effects of mercuric chloride on glycogen and protein content of Zebra fish, Danio rerio. Journal of Environmental Biology, 34(2): 277-281.
• Wehnekamp, F., Gabriela, G., Brauchle, C., Misgeld, T. & Lamb, D.C. 2014. 3D Real-Time Orbital Tracking Microscopy in Zebra Fish Embryos. Biophysical Journal, 106(2): 23a-23a.
• Ylieff, M.Y. & Poncin, P. 2003. Quantifying spontaneous swimming activity in fish with a computerized color video tracking system, a laboratory device using last imaging techniques. Fish Physiology and Biochemistry, 28(1-4): 281-282.