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INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Year 2018, Volume: 19 Issue: 2, 147 - 157, 15.10.2018
https://doi.org/10.23902/trkjnat.433329

Abstract

In this study, intra-specific variations in
naturally growing and cultivated mastic tree (Pistacia lentiscus L.) samples obtained from western parts of
Turkey were examined using ISSR and IRAP marker techniques. Samples from Crete
and Chios were also included in the study. Morphological measurements of some
leaf characteristics of the samples were performed and the
measured data was evaluated
statistically with a Pearson Correlation analysis to reveal the correlations
between character pairs. ISSR primers produced 81 bands between 161-1884bp with
96.3% polymorphism and IRAP primers produced 72 bands between
124-2027bp with 91.67% polymorphism. Polymorphism information content (PIC)
values were 0.458 and 0.418 for ISSR and IRAP, respectively. Genetic similarity
matrix was examined with Jaccard’s coefficient. Maximum similarity was found
between the Cretan samples (LG2 and LG3) with the ISSR analysis (0.933) and
between L25A (C1, Bodrum) and L29A (C1, Milas) with the IRAP analysis (0.593).
Unweighted pair group method with arithmetic mean (UPGMA) dendrogram was
divided into 12 and 4 groups by ISSR and IRAP methods, respectively. Specimens
were segregated on 3 main different clusters by the Principal Component
Analysis (PCA) based on the combined marker systems. The results showed that P. lentiscus has very high ratios of
intraspecific variation. The present work is an original study in terms of
large sampling including wild genotypes, cultivated specimen, Chios and Cretan
varieties, use of ISSR and IRAP combination, determination of relations between
culture and wild genotypes and the use of Bagy-1
retrotransposons in intraspecific polymorphism. This study may be considered as
a reference study for studies on gene pools of P. lentiscus and phylogenetic relationships within the species and
may contribute to species concept and agricultural breeding programs.

References

  • 1. Abuduli, A., Aydin, Y., Sakiroglu, M., Onay, A., Ercisli, S. & Altinkut Uncuoglu, A. 2016. Molecular Evaluation of Genetic Diversity in Wild-Type Mastic Tree (Pistacia lentiscus L.). Biochemical Genetics, https://doi.org/10.1007/s10528-016-9742-0
  • 2. Ak, B.E. & Parlakçı, H. 2009. Pistacia lentiscus in the Mediterranean Region in Turkey. Acta Horticulturae, 818: 77-81.
  • 3. AL-Saghir, M.G. 2010. Phylogenetic Analysis of the Genus Pistacia L. (Anacardiaceae) Based on Morphological Data. Asian Journal of Plant Sciences, 9(1): 28-35.
  • 4. Amirbakhtiar, N. & Sorkheh, K. 2015. DNA fingerprinting of wild Pistacia L. species germplasm in Iran using interretrotransposon amplified polymorphism (IRAP) marker. 1st Intertational and 9th National Biotechnology Congress of Islamic Republic of Iran 24-25 May 2015, Tehran, Iran.
  • 5. Balan, K.V., Prince, J., Han, Z., Dimas, K., Cladaras, M., Wyche, J.H., Sitaras, N.M. & Pantazis, P. 2007. Antiproliferative activity and induction of apoptosis in human colon cancer cells treated in vitro with constituents of a product derived from Pistacia lentiscus L. var. chia. Phytomedicine, 14(4): 263-272. https://doi.org/ 10.1016/j.phymed.2006.03.009
  • 6. Barazani, O., Dudai, N. & Golan-Goldhirsh, A. 2003. Comparison of Mediterranean Pistacia lentiscus genotypes by random amplified polymorphic DNA, chemical, and morphological analyses. Journal of Chemical Ecology, 29(8): 1939-1952. https://doi.org/10.1023/A:1024862614345
  • 7. Bhat, K.V. 2002. Molecular data analysis, Proceedings of the short-term training course on molecular marker application in plant breeding. ICAR, New Delhi.
  • 8. Botstein, D., White, R.L., Skolnick, M. & Davis, R.W. 1980. Construction of a genetic linkage map using restriction fragment length polymorphisms. The American Society of Human Genetics, 32: 314-331.
  • 9. Correia, O.A. & Catarino, F.M. 1994. Seasonal changes in soil-to-leaf resistance in Cistus sp. and Pistacia lentiscus. Acta Oecologica, 15: 289-300.
  • 10. Fan, S., Xiao, Z., Tang, X., Chen, C., Zhang, Y., Deng, Q., Yao, P. & Li, W. 2014. Inhibition effect of secondary metabolites accumulated in a pervaporation membrane bioreactor on ethanol fermentation of Saccharomyces cerevisiae. Bioresource Technology, 162: 8-13. https://doi.org/10.1016/j.biortech.2014.03.140
  • 11. Freudenreich, C.H., Stavenhagen, J.B. & Zakian, V.A. 1997. Stability of a CTG/CAG trinucleotide repeat in yeast is dependent on its orientation in the genome. Molecular and Cellular Biology, 17: 2090-2098.
  • 12. Fritz, U., Kiroký, P., Kami, H. & Wink, M. 2005. Environmentally caused dwarfism or a valid species - Is Testudo weissingeri Bour, 1996 a distinct evolutionary lineage? New evidence from mitochondrial and nuclear genomic markers. Molecular Phylogenetics and Evolution, 37(2): 389-401. https://doi.org/10.1016/j.ympev.2005.03.007
  • 13. Ghaemmaghami, L., Attar, F. & Rahiminejad, M. 2013. Distinctness and Inter Relationships of Pistacia L. Species in Iran as Evidenced by Retroelement Insertional Polymorphisms (IRAP Method). Iranian Journal of Botany, 19: 78-85.
  • 14. Golan-Goldrish, A., Barazani, O., Wang, Z.S., Khadka, D.K., Saunders, J.A., Kostiukovsky, V. & Rowland, L.J. 2004. Genetic relationships among Mediterranean Pistacia species evaluated by RAPD and AFLP markers. Plant Systematics and Evolution, 246: 9-18.
  • 15. Gribbon, B.M., Pearce, S.R., Kalendar, R., Schulman, A.H., Paulin, L., Jack, P.L., Kumar, A. & Flavell, A.J. 1999. Phylogeny and transpositional activity of Ty1-copia group retrotransposons in cereal genomes. Molecular Genetics and Genomics, 261: 883-891.
  • 16. He, M.L., Li, A., Xu, C.S., Wang, S.L., Zhang, M.L., Gu, H., Yang, Y.Q. & Tao, H.H. 2007. Mechanism of antiprostate cancer by gum mastic: NF-kappaB signal as target. Acta Pharmacologica Sinica, 28: 446-462.
  • 17. Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bulletin de la Societe Vaudoise des Sciences Naturelles, 44: 223-270.
  • 18. Kafkas, S. 2006. Phylogenetic analysis of the genus Pistacia by AFLP markers. Plant Systematics and Evolution, 262: 113-124.
  • 19. Kafkas, S. & Perl-Treves, R. 2001. Morphological and molecular phylogeny of Pistacia species in Turkey. Theoretical and Applied Genetics, 102(2001): 908-915. https://doi.org/10.1007/s001220000526
  • 20. Kafkas, S. & Perl-Treves, R. 2002. Interspecific Relationships in Pistacia Based on RAPD Fingerprint. HortScience, 37(1): 168-171.
  • 21. Kalendar, R., Flavell, A.J., Ellis, T.H.N., Sjakste, T., Moisy, C. & Schulman, A.H. 2011. Analysis of plant diversity with retrotransposon-based molecular markers. Heredity, 106(4): 520-530. https://doi.org/10.1038/hdy.2010.93
  • 22. Kalendar, R., Grob, T., Regina, M., Suoniemi, A. & Schulman, A.H. 1999. IRAP and REMAP: two new retrotransposon-based DNA fingerprinting techniques. Theoretical and Applied Genetics, 98: 704-711.
  • 23. Kılınç, F.M., Süzerer, V., Çiftçi, Y.Ö., Onay, A., Yıldırım, H., Altınkut-Uncuoğlu, A., Tilkat, E., Koç, İ., Akdemir, Ö.F. & Karakaş-Metin, Ö. 2014. Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers. Plant Growth Regulation, 75(1): 75-88.
  • 24. Kırdök, E. & Çiftçi, Y.Ö. 2016. Retrotransposon marker systems as a tool to analyze molecular diversity of Mediterranean Pistacia species. International Journal of Agriculture and Biology, 18: 601-606.
  • 25. Koç, İ., Akdemir, H., Onay, H., Çiftçi, Y. 2014. Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets. Acta Physiologiae Plantarum, 36: 2373-2384. https://doi.org/10.1007/s11738-014-1610-0
  • 26. Kokwaro, J.O. & Gillet, J.B. 1980. Notes on the Anacardiaceae of Eastern Africa. Kew Bull, 34(1980): 745-760.
  • 27. Kolano, B., Bednara, E. & Weiss-Schneeweiss, H. 2013. Isolation and characterization of reverse transcriptase fragments of LTR retrotransposons from the genome of Chenopodium quinoa (Amaranthaceae). Plant Cell Reports, 32(10): 1575-1588. https://doi.org/10.1007/s00299-013-1468-4.
  • 28. Lodhi, M.A., Ye, G.N., Weeden, N.F. & Reisch, B.I. 1994. A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Reporter, 12(1): 6-13. https://doi.org/10.1007/BF02668658
  • 29. Loutrari, H., Magkouta, S., Pyriochou, A., Koika, V., Kolisis, F.N., Papapetropoulos, A. & Roussos, C. 2006. Mastic oil from Pistacia lentiscus var. chia inhibits growth and survival of human K562 leukemia cells and attenuates angiogenesis. Nutrition and Cancer, 55(1): 86-93. https://doi.org/10.1207/s15327914nc5501_11
  • 30. Ma, D., Amonlirdviman, K., Raffard, R.L., Abate, A., Tomlin, C.J. & Axelrod, J.D. 2008. Cell packing influences planar cell polarity signaling. Proceedings of the National Academy of Sciences USA, 105(48): 18800-18805.
  • 31. Nagy, Z.T., Joger, U., Guicking, D. & Wink, M. 2003. Phylogeography of the European whip snake Coluber (Hierophis) viridiflavus as inferred from nucleotide sequences of the mitochondrial cytochrome-b gene and ISSR genomic fingerprinting. Biota, 3: 109-118.
  • 32. Parfitt, D.E. & Badenes, M.L. 1997. Phylogeny of the genus Pistacia as determined from analysis of the chloroplast genome. PNAS, 94 (1997): 7987-7992.
  • 33. Park, J.K., Liu, X., Strauss, T.J., McKearin, D.M. & Liu, Q. 2007. The miRNA pathway intrinsically controls self-renewal of Drosophila germline stem cells. Current Biology, 17(6): 533-538. https://doi.org/10.1016/j.cub.2007.01.060
  • 34. Reddy, M.P., Sarla, N. & Siddiq, E.A. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9-17. https://doi.org/10.1023/A:1020691618797
  • 35. Sorkheh, K., Amirbakhtiar, N. & Ercisli, S. 2016. Potential Start Codon Targeted (SCoT) and Interretrotransposon Amplified Polymorphism (IRAP) Markers for Evaluation of Genetic Diversity and Conservation of Wild Pistacia Species Population. Biochemical Genetics, https://doi.org/10.1007/s10528-016-9725-1
  • 36. Stevens, P.F. 2008. Angiosperm phylogeny website. Version 9(2008): 1-2.
  • 37. Tenaillon, M.I., Hufford, M.B., Gaut, B.S. & Ross-Ibarra, J. 2011. Genome Size and Transposable Element Content as Determined by High-Throughput Sequencing in Maize and Zea luxurians. Genome Biology and Evolution, 3: 219-229. https://doi.org/10.1093/gbe/evr008
  • 38. Whitehouse, W.E. 1957. The Pistachio nut- a new crop for the western United States. Economic Botany, 11(4): 281-321.
  • 39. Yaltirik, F. 1967. Pistacia L., Flora of Turkey, In: Davis, P.H. (ed.), Cilt 2, Edinburgh At The University Press, Edinburgh, 554 pp.
  • 40. Zografou, P., Linos, A. & Hagidimitriou, M. 2010. Genetic diversity among different genotypes of Pistacia lentiscus var. chia (mastic tree). Options Méditerranéennes, 94: 159-163.
  • 41. Zohary, M. 1952. A monographical study of the genus Pistacia. Palestine Journal of Botany, 5(4): 187-228.

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Year 2018, Volume: 19 Issue: 2, 147 - 157, 15.10.2018
https://doi.org/10.23902/trkjnat.433329

Abstract

Bu
çalışmada, ISSR ve IRAP markör teknikleri kullanılarak Türkiye’nin batı
kesiminde doğal olarak yetişen ve kültürü yapılan sakız ağacı (Pistacia lentiscus L.) örneklerindeki
tür içi varyasyon analizi yapılmıştır. Girit ve Sakız Adası örnekleri de
çalışmaya dahil edilmiştir. Bazı yaprak özelliklerinin morfolojik ölçümleri
gerçekleştirilmiş ve aralarındaki korelasyon Pearson Korelasyon analizi ile
belirlenmiştir. ISSR primerleri 161-1884bç arasında %96,3 polimorfizm ile 81
bant üretmiştir. IRAP primerleri ise 124-2027bç arasında %91,67 polimorfizm ile
72 bant üretmiştir. Polimorfizm bilgi içeriği (PIC) değerleri 0,458 (ISSR) ve
0,418 (IRAP) arasında bulunmuştur. Genetik benzerlik matriksleri Jaccard
katsayısıyla oluşturulmuştur. ISSR sonuçlarında en yüksek benzerlik Girit
örnekleri (LG2 ve LG3) arasında (0,933) ve IRAP sonuçlarında ise L25A (C1,
Bodrum) ve L29A (C1, Milas) arasında bulunmuştur. UPGMA yöntemiyle kurulan
dendrogramlarda sırasıyla ISSR için 12 grup ve IRAP için 4 grup ayrılmıştır.
Her iki markör sistemi için ortak kurulan temel bileşen analizi (PCA)
grafiğinde 3 farklı küme oluşmuştur. Sonuçlar göstermiştir ki, P. lentiscus yüksek oranlarda tür içi
varyasyona sahiptir. Bu çalışma, ISSR ve IRAP markörlerinin yabani genotipler,
kültür türleri, Sakız Adası ve Girit çeşitlerinin analizinde kullanımı, kültür
varyeteleri ve yabani genotipler arasındaki ilişkilerin belirlenmesi ve tür içi
polimorfizmde Bagy-1
retrotranspozonlarının kullanımı açısından özgün bir çalışmadır. Söz konusu
çalışma, P. lentiscus gen havuzunun
ve filogenetik ilişkilerinin araştırılması, tür sınırlarını saptamaya yönelik
yapılacak tarımsal ıslah çalışmaları için referans niteliğindedir.

References

  • 1. Abuduli, A., Aydin, Y., Sakiroglu, M., Onay, A., Ercisli, S. & Altinkut Uncuoglu, A. 2016. Molecular Evaluation of Genetic Diversity in Wild-Type Mastic Tree (Pistacia lentiscus L.). Biochemical Genetics, https://doi.org/10.1007/s10528-016-9742-0
  • 2. Ak, B.E. & Parlakçı, H. 2009. Pistacia lentiscus in the Mediterranean Region in Turkey. Acta Horticulturae, 818: 77-81.
  • 3. AL-Saghir, M.G. 2010. Phylogenetic Analysis of the Genus Pistacia L. (Anacardiaceae) Based on Morphological Data. Asian Journal of Plant Sciences, 9(1): 28-35.
  • 4. Amirbakhtiar, N. & Sorkheh, K. 2015. DNA fingerprinting of wild Pistacia L. species germplasm in Iran using interretrotransposon amplified polymorphism (IRAP) marker. 1st Intertational and 9th National Biotechnology Congress of Islamic Republic of Iran 24-25 May 2015, Tehran, Iran.
  • 5. Balan, K.V., Prince, J., Han, Z., Dimas, K., Cladaras, M., Wyche, J.H., Sitaras, N.M. & Pantazis, P. 2007. Antiproliferative activity and induction of apoptosis in human colon cancer cells treated in vitro with constituents of a product derived from Pistacia lentiscus L. var. chia. Phytomedicine, 14(4): 263-272. https://doi.org/ 10.1016/j.phymed.2006.03.009
  • 6. Barazani, O., Dudai, N. & Golan-Goldhirsh, A. 2003. Comparison of Mediterranean Pistacia lentiscus genotypes by random amplified polymorphic DNA, chemical, and morphological analyses. Journal of Chemical Ecology, 29(8): 1939-1952. https://doi.org/10.1023/A:1024862614345
  • 7. Bhat, K.V. 2002. Molecular data analysis, Proceedings of the short-term training course on molecular marker application in plant breeding. ICAR, New Delhi.
  • 8. Botstein, D., White, R.L., Skolnick, M. & Davis, R.W. 1980. Construction of a genetic linkage map using restriction fragment length polymorphisms. The American Society of Human Genetics, 32: 314-331.
  • 9. Correia, O.A. & Catarino, F.M. 1994. Seasonal changes in soil-to-leaf resistance in Cistus sp. and Pistacia lentiscus. Acta Oecologica, 15: 289-300.
  • 10. Fan, S., Xiao, Z., Tang, X., Chen, C., Zhang, Y., Deng, Q., Yao, P. & Li, W. 2014. Inhibition effect of secondary metabolites accumulated in a pervaporation membrane bioreactor on ethanol fermentation of Saccharomyces cerevisiae. Bioresource Technology, 162: 8-13. https://doi.org/10.1016/j.biortech.2014.03.140
  • 11. Freudenreich, C.H., Stavenhagen, J.B. & Zakian, V.A. 1997. Stability of a CTG/CAG trinucleotide repeat in yeast is dependent on its orientation in the genome. Molecular and Cellular Biology, 17: 2090-2098.
  • 12. Fritz, U., Kiroký, P., Kami, H. & Wink, M. 2005. Environmentally caused dwarfism or a valid species - Is Testudo weissingeri Bour, 1996 a distinct evolutionary lineage? New evidence from mitochondrial and nuclear genomic markers. Molecular Phylogenetics and Evolution, 37(2): 389-401. https://doi.org/10.1016/j.ympev.2005.03.007
  • 13. Ghaemmaghami, L., Attar, F. & Rahiminejad, M. 2013. Distinctness and Inter Relationships of Pistacia L. Species in Iran as Evidenced by Retroelement Insertional Polymorphisms (IRAP Method). Iranian Journal of Botany, 19: 78-85.
  • 14. Golan-Goldrish, A., Barazani, O., Wang, Z.S., Khadka, D.K., Saunders, J.A., Kostiukovsky, V. & Rowland, L.J. 2004. Genetic relationships among Mediterranean Pistacia species evaluated by RAPD and AFLP markers. Plant Systematics and Evolution, 246: 9-18.
  • 15. Gribbon, B.M., Pearce, S.R., Kalendar, R., Schulman, A.H., Paulin, L., Jack, P.L., Kumar, A. & Flavell, A.J. 1999. Phylogeny and transpositional activity of Ty1-copia group retrotransposons in cereal genomes. Molecular Genetics and Genomics, 261: 883-891.
  • 16. He, M.L., Li, A., Xu, C.S., Wang, S.L., Zhang, M.L., Gu, H., Yang, Y.Q. & Tao, H.H. 2007. Mechanism of antiprostate cancer by gum mastic: NF-kappaB signal as target. Acta Pharmacologica Sinica, 28: 446-462.
  • 17. Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bulletin de la Societe Vaudoise des Sciences Naturelles, 44: 223-270.
  • 18. Kafkas, S. 2006. Phylogenetic analysis of the genus Pistacia by AFLP markers. Plant Systematics and Evolution, 262: 113-124.
  • 19. Kafkas, S. & Perl-Treves, R. 2001. Morphological and molecular phylogeny of Pistacia species in Turkey. Theoretical and Applied Genetics, 102(2001): 908-915. https://doi.org/10.1007/s001220000526
  • 20. Kafkas, S. & Perl-Treves, R. 2002. Interspecific Relationships in Pistacia Based on RAPD Fingerprint. HortScience, 37(1): 168-171.
  • 21. Kalendar, R., Flavell, A.J., Ellis, T.H.N., Sjakste, T., Moisy, C. & Schulman, A.H. 2011. Analysis of plant diversity with retrotransposon-based molecular markers. Heredity, 106(4): 520-530. https://doi.org/10.1038/hdy.2010.93
  • 22. Kalendar, R., Grob, T., Regina, M., Suoniemi, A. & Schulman, A.H. 1999. IRAP and REMAP: two new retrotransposon-based DNA fingerprinting techniques. Theoretical and Applied Genetics, 98: 704-711.
  • 23. Kılınç, F.M., Süzerer, V., Çiftçi, Y.Ö., Onay, A., Yıldırım, H., Altınkut-Uncuoğlu, A., Tilkat, E., Koç, İ., Akdemir, Ö.F. & Karakaş-Metin, Ö. 2014. Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers. Plant Growth Regulation, 75(1): 75-88.
  • 24. Kırdök, E. & Çiftçi, Y.Ö. 2016. Retrotransposon marker systems as a tool to analyze molecular diversity of Mediterranean Pistacia species. International Journal of Agriculture and Biology, 18: 601-606.
  • 25. Koç, İ., Akdemir, H., Onay, H., Çiftçi, Y. 2014. Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets. Acta Physiologiae Plantarum, 36: 2373-2384. https://doi.org/10.1007/s11738-014-1610-0
  • 26. Kokwaro, J.O. & Gillet, J.B. 1980. Notes on the Anacardiaceae of Eastern Africa. Kew Bull, 34(1980): 745-760.
  • 27. Kolano, B., Bednara, E. & Weiss-Schneeweiss, H. 2013. Isolation and characterization of reverse transcriptase fragments of LTR retrotransposons from the genome of Chenopodium quinoa (Amaranthaceae). Plant Cell Reports, 32(10): 1575-1588. https://doi.org/10.1007/s00299-013-1468-4.
  • 28. Lodhi, M.A., Ye, G.N., Weeden, N.F. & Reisch, B.I. 1994. A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Reporter, 12(1): 6-13. https://doi.org/10.1007/BF02668658
  • 29. Loutrari, H., Magkouta, S., Pyriochou, A., Koika, V., Kolisis, F.N., Papapetropoulos, A. & Roussos, C. 2006. Mastic oil from Pistacia lentiscus var. chia inhibits growth and survival of human K562 leukemia cells and attenuates angiogenesis. Nutrition and Cancer, 55(1): 86-93. https://doi.org/10.1207/s15327914nc5501_11
  • 30. Ma, D., Amonlirdviman, K., Raffard, R.L., Abate, A., Tomlin, C.J. & Axelrod, J.D. 2008. Cell packing influences planar cell polarity signaling. Proceedings of the National Academy of Sciences USA, 105(48): 18800-18805.
  • 31. Nagy, Z.T., Joger, U., Guicking, D. & Wink, M. 2003. Phylogeography of the European whip snake Coluber (Hierophis) viridiflavus as inferred from nucleotide sequences of the mitochondrial cytochrome-b gene and ISSR genomic fingerprinting. Biota, 3: 109-118.
  • 32. Parfitt, D.E. & Badenes, M.L. 1997. Phylogeny of the genus Pistacia as determined from analysis of the chloroplast genome. PNAS, 94 (1997): 7987-7992.
  • 33. Park, J.K., Liu, X., Strauss, T.J., McKearin, D.M. & Liu, Q. 2007. The miRNA pathway intrinsically controls self-renewal of Drosophila germline stem cells. Current Biology, 17(6): 533-538. https://doi.org/10.1016/j.cub.2007.01.060
  • 34. Reddy, M.P., Sarla, N. & Siddiq, E.A. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9-17. https://doi.org/10.1023/A:1020691618797
  • 35. Sorkheh, K., Amirbakhtiar, N. & Ercisli, S. 2016. Potential Start Codon Targeted (SCoT) and Interretrotransposon Amplified Polymorphism (IRAP) Markers for Evaluation of Genetic Diversity and Conservation of Wild Pistacia Species Population. Biochemical Genetics, https://doi.org/10.1007/s10528-016-9725-1
  • 36. Stevens, P.F. 2008. Angiosperm phylogeny website. Version 9(2008): 1-2.
  • 37. Tenaillon, M.I., Hufford, M.B., Gaut, B.S. & Ross-Ibarra, J. 2011. Genome Size and Transposable Element Content as Determined by High-Throughput Sequencing in Maize and Zea luxurians. Genome Biology and Evolution, 3: 219-229. https://doi.org/10.1093/gbe/evr008
  • 38. Whitehouse, W.E. 1957. The Pistachio nut- a new crop for the western United States. Economic Botany, 11(4): 281-321.
  • 39. Yaltirik, F. 1967. Pistacia L., Flora of Turkey, In: Davis, P.H. (ed.), Cilt 2, Edinburgh At The University Press, Edinburgh, 554 pp.
  • 40. Zografou, P., Linos, A. & Hagidimitriou, M. 2010. Genetic diversity among different genotypes of Pistacia lentiscus var. chia (mastic tree). Options Méditerranéennes, 94: 159-163.
  • 41. Zohary, M. 1952. A monographical study of the genus Pistacia. Palestine Journal of Botany, 5(4): 187-228.
There are 41 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Article/Araştırma Makalesi
Authors

Pelin Turhan Serttaş 0000-0001-8617-7437

Tamer Özcan This is me

Publication Date October 15, 2018
Submission Date June 12, 2018
Acceptance Date September 20, 2018
Published in Issue Year 2018 Volume: 19 Issue: 2

Cite

APA Turhan Serttaş, P., & Özcan, T. (2018). INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY. Trakya University Journal of Natural Sciences, 19(2), 147-157. https://doi.org/10.23902/trkjnat.433329
AMA Turhan Serttaş P, Özcan T. INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY. Trakya Univ J Nat Sci. October 2018;19(2):147-157. doi:10.23902/trkjnat.433329
Chicago Turhan Serttaş, Pelin, and Tamer Özcan. “INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia Lentiscus L.) FROM TURKEY”. Trakya University Journal of Natural Sciences 19, no. 2 (October 2018): 147-57. https://doi.org/10.23902/trkjnat.433329.
EndNote Turhan Serttaş P, Özcan T (October 1, 2018) INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY. Trakya University Journal of Natural Sciences 19 2 147–157.
IEEE P. Turhan Serttaş and T. Özcan, “INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY”, Trakya Univ J Nat Sci, vol. 19, no. 2, pp. 147–157, 2018, doi: 10.23902/trkjnat.433329.
ISNAD Turhan Serttaş, Pelin - Özcan, Tamer. “INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia Lentiscus L.) FROM TURKEY”. Trakya University Journal of Natural Sciences 19/2 (October 2018), 147-157. https://doi.org/10.23902/trkjnat.433329.
JAMA Turhan Serttaş P, Özcan T. INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY. Trakya Univ J Nat Sci. 2018;19:147–157.
MLA Turhan Serttaş, Pelin and Tamer Özcan. “INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia Lentiscus L.) FROM TURKEY”. Trakya University Journal of Natural Sciences, vol. 19, no. 2, 2018, pp. 147-5, doi:10.23902/trkjnat.433329.
Vancouver Turhan Serttaş P, Özcan T. INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY. Trakya Univ J Nat Sci. 2018;19(2):147-5.

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