In Silico and Molecular Analysis of Some Mosaic Diseases on Cucurbit Plants in Iğdır Province, TÜrkiye

Year 2024, Volume: 11 Issue: 1, 90 - 103, 28.01.2024

Abdullah Güller , Mustafa Usta , Gülüstan Korkmaz , Serap Demirel

https://doi.org/10.30910/turkjans.1387512

Cited By: 1

Abstract

Cucumber mosaic cucumovirus (CMV) and Watermelon mosaic potyvirus (WMV) are plant viruses with positive single-stranded RNA genome that infect cucurbits and cause economic losses. Both viruses cause especially mosaic pattern and deformities in leaves, in cucurbit plants. The leaves of 23 melon and 28 watermelon plants showing such symptoms were sampled from different regions of Iğdır province. Samples with and without symptoms were tested by the Reverse Transcription Polymerase Chain Reaction (RT-PCR) using coat protein gene (CP)-specific primers. Polymerization tests amplified DNA fragments of the expected size for CMV and WMV. Some fragments with positive results were purified, bacterial cloned, nucleotide sequences revealed and registered in the GeneBank (NCBI). Sequence analyzes showed that it contained 593 bp and 822 bp for CMV and WMV, corresponding to the partial CP gene. Phylogenetic relationships with isolates from different geographical regions and plant material were investigated for both viruses. The generated phylogenetic tree confirmed that CMV-Iğdır isolate was in Group I and subgroup B, and WMV-Iğdır isolates were in different groups. In addition, coat proteins of virus isolates were characterized by in silico tools.
In the current study, the presence WMV of and CMV in watermelon and melon was determined for the first time in Iğdır province, and group/subgroup assignments of CMV were revealed.

Keywords

Cucumber mosaic cucumovirus, In silico analysis, Molecular characterization, RT-PCR, Watermelon mosaic potyvirus

Project Number

Çalışmanın projesi bulunmamaktadır

Iğdir İli Kabakgil Bitkilerinde Bazı Mozaik Hastalıklarının In Siliko ve Moleküler Analizi

Abstract

Cucumber mosaic cucumovirus (CMV) ve Watermelon mosaic potyvirus (WMV) kabakgilleri infekteleyen ve ekonomik kayıplara neden pozitif tek iplikli RNA genomuna sahip bitki virüslerdir. Her iki virüs kabakgil bitkilerde başta mozaik deseni ve yapraklarda şekil bozukluklarına yol açar. Bu tür belirtiler gösteren 23 kavun ve 28 karpuz bitkisinin yaprakları Iğdır ilinin farklı bölgelerinden örneklendirilmiştir. Simptomlu ve simptomsuz örnekler Reverse Transkripsiyon Polimeraz Zircir Reaksiyonu (RT-PCR) tarafından kılıf protein geni (CP) spesifik primerler kullanılarak testlenmiştir. Polimerizasyon testleri, CMV ve WMV için beklenen boyutta DNA fragmentleri amplifiye etmiştir. Pozitif sonuçlu bazı fragmentler saflaştırılmış, bakteriyel klonlaması gerçekleştirilmiş, nükleotit dizileri ortaya çıkarılmış ve gen bankasına (NCBI) kaydedilmiştir. Dizi analizleri, CMV ve WMV’nin kısmi kılıf protein dizilerine karşılık gelen 593 bp ve 822 bp nükleotit içerdiğini göstermiştir. Her iki virüs için farklı coğrafi bölgelerden sağlanan izolatlar nükleotit sekanslarına dayalı olarak filogenetik ilişkileri ortaya konmuştur. Oluşturulan filogenetik ağaç CMV-Iğdır izolatının Grup I ve alt grup B’de olduğunu ve WMV-Iğdır izolatlarının ise farklı gruplarda yer aldığını doğrulamıştır. Ayrıca virus izolatlarının kılıf proteinleri in silico araçlar ile karakterize edilmiştir.
Bu çalışmayla Iğdır ilinde moleküler olarak ilk kez kavunda ve karpuzda CMV ile WMV varlığı saptanmış ve CMV’nin grup/altgrup atamaları gerçekleştirilmiştir.

Keywords

Hıyar mozaik virüsü, In silico analiz, Karpuz mozaik virüsü, Moleküler karaterizasyon, RT-PCR

Ethical Statement

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Supporting Institution

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Project Number

Çalışmanın projesi bulunmamaktadır

Thanks

Dergi yönteici ve sekreteryasına teşekkür ediyorum

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