Türkiye Tarımsal Araştırmalar Dergisi

2148-2306 2528-858X

Siirt University

10.19159/tutad.1328123

Phytopathology

Fitopatoloji

Investigation of Fungal Causal Agents Root Rot and Wilt on Pistachio (Pistacia vera L.) Orchard: Siirt Province Location, Türkiye

Antepfıstığı (Pistacia vera L.) Bahçelerinde Kök Çürüklüğü ve Solgunluğa Neden Olan Fungal Etmenlerin Araştırılması: Siirt İli Lokasyonu, Türkiye

https://orcid.org/0000-0003-3135-4621

Aydın

Mehmet Hadi

Siirt Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Siirt, TÜRKİYE

https://orcid.org/0000-0003-2215-2710

İnal

Behcet

SİİRT ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ

https://orcid.org/0000-0003-2625-0684

Uzun

Tuba

Siirt Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Siirt, TÜRKİYE

https://orcid.org/0000-0002-6185-9359

Aydın

Yusuf

Gaziantep Üniversitesi, Nizip Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümü, Nizip-Gaziantep, TÜRKİYE

https://orcid.org/0000-0002-5205-1534

Kayalar

Berrin

Siirt Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Siirt, TÜRKİYE

11 27 2023

10 3 308 319 07 16 2023 10 24 2023

2014

Turkish Journal of Agricultural Research

In recent years, symptoms such as root rot and wilt have been observed in pistachio (Pistacia vera L.) orchards in this province, and drying of trees has been detected. This study investigated soil-borne fungal pathogens that cause diseases in crops. Survey studies were carried out according to the controlled sampling survey method in Siirt province center, Eruh, Şirvan, Kurtalan districts and villages in 2021-2022. Fungal species were determined based on morphological characters such as color, growth in nutrient media, and spore structures, and by matching analysis of Internal Transcribed Spacer (ITS) gene sequences with other genes in the National Center for Biotechnology Information (NCBI) gene bank. Pathogenicity study was carried out on one-year-old Siirt variety in the greenhouse and pathogenic isolates were determined. As a result of the study, mostly Fusarium species (F. solani, F. oxysporum, F. verticillioides, F. equseti, F. acuminatum, F. avenaceaum, F. proliferatum, F. brchygibbosum, Fusarium spp.) and Diaporthe hongkongensis, Scydalidium sp., Rosellinia sp., Nectria diminuta, Neoscytalidium dimitatum, Rhizoctonia solani, Macrophomina phaseolina, Phoma spp., and Cylindrocarpon spp. were isolated and some of them found to be pathogenic at different rates.

Antepfıstığı (Pistacia vera L.) bahçelerinde son yıllarda kök çürüklüğü ve solgunluk gibi belirtiler gözlemlenmiş ve ağaçlarda kurumalar tespit edilmiştir. Bu çalışmada kurumalara neden olan toprak kökenli fungal patojenler araştırılmıştır. Çalışma; Siirt ili Merkez, Eruh, Şirvan, Kurtalan ilçeleri ve köylerinde 2021-2022 yıllarında güdümlü örnekleme sörvey metoduna göre yürütülmüştür. Elde edilen fungus türleri, renk, gelişim durumları, spor yapıları gibi morfolojik karakterlerine ve ITS (Internal Transcribed Spacer) gen dizilerinin NCBI (The National Center for Biotechnology Information) gen bankasındaki diğer genlerle eşleştirme analizleri ile belirlenmiştir. Patojenisite çalışması, bir yaşındaki Siirt çeşidi üzerinde yapılmış ve patojen izolatlar belirlenmiştir. Çalışma sonucunda ağırlıklı olarak Fusarium türleri (F. solani, F. oxysporum, F. verticillioides, F. equseti, F. acuminatum, F. avenaceaum, F. proliferatum, F. brchygibbosum, Fusarium spp.) ile Diaporthe hongkongensis, Scydalidium sp., Rosellinia sp., Nectria diminuta, Neoscytalidium dimitatum, Rhizoctonia solani, Macrophomina phaseolina, Phoma spp. ve Cylindrocarpon spp., izole edilmiş ve bunların farklı oranlarda patojen oldukları tespit edilmiştir.

Antepfıstığı toprak patojenleri polimeraz zincir reaksiyonu Siirt

Pistachio soil pathogens polymerase chain reaction Siirt

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