Süt, Et, İdrar ve Yemde Altı Farklı Grup Antimikrobiyal İlacın Biochip Array-Based Immunoassay ile Aynı Anda Tespiti

Year 2012, Volume: 31 Issue: 2, 29 - 33, 01.06.2012

Hasan H. Oruç Wilson K. Rumbeıha Steve Ensley Dwayne E. Schrunk

Abstract

Antimikrobiyaller besin üretiminde faydalandığımız hayvanlarda yaygın olarak kullanılmaktadır. Bu nedenle, bu besinlerde bulunabilen antimikrobiyal kalıntıları insan sağlığı açısından önemlidir. Farklı antimikrobiyal kalıntılarının aynı numunede ve aynı anda birlikte tespiti önemlidir. Bu nedenle, bu çalışmanın amacı Biochip array-based immunoassay yöntemi ile altı farklı grup antibakteriyel ilacın süt, et, idrar ve yemde deneysel şartlarda tespit etmektir. Bu test, aynı anda altı farklı grup antimikrobiyalin süt, idrar, et, bal ve yem numunelerinde miktarlarının belirlenmesinde kullanılmaktadır. Bu metodla hedef ilaçlar olan norfloksasin, seftifor, florfenikol, streptomisin, taylosin ve tetrasiklin süt, et, idrar ve yemde tespit edildi. Sonuç olarak, bu test ile yukarıda belirtilen altı farklı gruptaki hedef antibakteriyel ilaç süt, et, idrar ve yemde etkili bir şekilde tespit edilebilmektedir. Bu metod, belirtilen numunelerde bu antibakteriyel ilaçların kalıntılarının rutin analizinde kullanılabilir

Keywords

Antimikrobiyaller, biochip array-based immunoassay, süt, et, idrar, yem

Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices

Abstract

Antimicrobials are widely used for animal health in food-producing animals. Therefore, antimicrobial residues are of food safety concern. Simultaneous detection of different antimicrobial residues in the same matrice is important. Therefore, the objective of this study was to detect six different group antimicrobials in milk, meat, urine, and feed matrices under experimental conditions, and to evaluate its use for routine analysis. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different groups of six antimicrobials in milk, urine, meat, honey, and feed. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in milk, meat, urine, and in feed matrices. In conclusion, this assay can effectively detect all target antibacterials from different groups in milk, meat, urine, and feed matrices, and this assay can be used for routine detection of these antibacterial residues in the stated matrices.

Keywords

Antimicrobials, biochip array-based immunoassay, milk, meat, urine, feed

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