Kollajen/Jelatin/Bal Esaslı Çift Katmanlı Doku İskelesi Üretimi ve Karakterizasyonu

Year 2022, Volume: 14 Issue: 2, 619 - 631, 31.07.2022

Yaren Erdem , Sahra Ezgi Süngü , Ömer Aktürk

https://doi.org/10.29137/umagd.1079714

Abstract

Bu projede, doğal, biyouyumlu ve biyobozunur polimerler olan kollajen/jelatin karışımından liyofilizasyon yoluyla gözenekli bir doku iskelesi üretilmiş, daha sonra bu katman üstüne elektro-eğirme yöntemi ile jelatin/polietilen oksit (PEO)/bal karışımıyla nanofibröz bir tabaka biriktirilmiştir. Doku iskelesi, gluteraldehit buharı ile muamele edilip sonrasında EDC/NHS kimyasallarıyla çapraz bağlanmıştır. Karakterizasyon için; Fourier Dönüşümlü Kızılötesi (FTIR) spektroskopisi, Termogravimetrik analiz (TGA), Taramalı Elektron Mikroskopisi (SEM), gözenek boyut dağılımı testi ve sulu ortamda degredasyon testleri yapılmıştır. Liyofilize katman, 1:1 (w/w) kollajen/jelatin karışımıyla üretilip, üst tabaka ise jelatin/PEO/bal karışımlarının en uygun oranı (2:2:2 w/w, %6 w/v toplam malzeme) seçilerek elektro-eğirme yöntemiyle bu katman üstüne toplanmıştır. İskelenin liyofilize katmanı 5−200 µm arasında geniş bir gözenek boyut dağılımına sahiptir. Çapraz bağlamadan sonra, gözenek boyut dağılımı (30−40 µm civarında yoğunlaşarak) daha homojen hale gelmiştir. SEM analizine göre, düzenli bir fiber boyut dağılımı (Dort = 423 ± 85 nm) elde edilmiş ve çapraz bağlama ve yıkama işlemlerinden sonra az miktarda fiber kaynaşması meydana gelmiştir. TGA ve degredasyon sonuçlarına göre, çapraz bağlama sonrasında iskele sağlamlığı artmıştır. Sonuç olarak, geliştirilen doku iskelesi, sahip olduğu sağlam, gözenekli ve fiberli yapısıyla farklı doku mühendisliği uygulamaları için uygun bir aday olabilir.

Keywords

Kollajen, Jelatin, Bal, Doku iskelesi, Elektro-Eğirme

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

Başvuru No: 1919B011901535

Thanks

Bu proje, TÜBİTAK 2209-A Üniversite Ögrencileri Arastırma Projeleri Destekleme Programı kapsamında desteklenmiştir. Projedeki yardımlarından ötürü Raziye Koraş ve Baraa Mehyo'ya teşekkürlerimizi sunarız.

Fabrication of Double-layered Tissue Scaffolds with Collagen/Gelatin/Honey Blends and Its Characterization

Abstract

In this project, a porous tissue scaffold composed of collagen/gelatin, which are natural, biocompatible, and biodegradable polymers, was fabricated by lyophilization, then a nanofibrous gelatin/polyethylene oxide (PEO)/honey blend was accumulated onto this layer via the electro-spinning process. The tissue scaffold was cross-linked by treating with glutaraldehyde vapor followed by EDC/NHS reagents. For the characterization, Fourier Transformed Infrared (FTIR) spectroscopy, Thermogravimetric Analysis (TGA), Scanning Electron Microscopy (SEM), pore size distribution analysis, and aqueous degradation tests were performed. While the lyophilized layer was fabricated by 1:1 (w/w) collagen/gelatin mixture, the top layer was electro-spun onto this layer by selecting the most appropriate blend ratio (2:2:2 w/w, %6 w/v total material). The lyophilized scaffold layer had a wide pore size distribution in the 5−200 µm range. After the cross-linking, pore size distribution became more homogenous (concentrating around 30−40 µm). According to SEM analysis, a uniform fiber size distribution (Dave = 423 ± 85 nm) was obtained and after the cross-linking and rinsing processes a slight fiber fusion occurred. Regarding the TGA and degradation results, the scaffold robustness increased after the cross-linking. Overall, the developed tissue scaffold with its stable, porous and fibrous form could be a suitable candidate for different tissue engineering applications.

Keywords

Collagen, Gelatin, Honey, Tissue scaffold, electro-spinning

Project Number

Başvuru No: 1919B011901535

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