Tıbbi Öneme Sahip Streptomyces Bakterilerinin Farklı Besiyerlerinde Çoğalma Oranlarının Belirlenmesi

Year 2021, Volume: 14 Issue: 3, 304 - 311, 30.12.2021

Hamdullah Seçkin , Kerem Özdemir

https://doi.org/10.52976/vansaglik.933104

Cited By: 1

Abstract

Aim: A total of 24 sediment samples were taken from the rivers flowing into Van Lake and their growth rates were examined in three different media. In our study, we aimed to investigate which Streptomyces (medically important) bacteria reproduce more in different medium.
Material-Method: In the study, a total of 24 sediment samples were taken from the rivers pouring into Van Lake. Then the moisture and pH of the samples were determined. Streptomyces bacteria were isolated from the samples taken and pure cultures of the bacteria were obtained. During the isolation process, three different media, Bennet's Agar, Medium 65 and SM3 Agar, were used.
Results: At the end of the isolation process, a total of 179 Streptomyces bacteria were isolated, 145 in Bennet's Agar medium, 8 in SM3 medium and 26 in Medium 65 medium, according to the counting process performed according to the morphological condition after the isolation process.
Conclusion: As a result, it was determined that Streptomyces bacteria isolated from sediment samples taken from fresh water grew mostly in Bennet's Agar medium among Bennet's Agar, SM3, and Medium 65 media. It is thought that Bennet's Agar medium can be used for the identification of new Streptomyces species producing secondary metabolites from aquatic environments.

Keywords

Streptomyces, Bennet, Medium 65, SM3 Agar, Van Lake

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Project Number

2017-FDK-5054

Determination of the Growth Rate of Medicinally Important Streptomyces Bacteria in Different Media

Abstract

Objective: A total of 24 sediment samples were taken from the rivers flowing into Van Lake and their growth rates were examined in three different media. In our study, we aimed to investigate which Streptomyces (medi-cally important) bacteria reproduce more in different medium.
Material and Method: In the study, a total of 24 sediment samples were taken from the rivers pouring into Van Lake. Then the moisture and pH of the samples were determined. Streptomyces bacteria were isolated from the samples taken and pure cultures of the bacteria were obtained. During the isolation process, three different me-dia, Modified Bennett's Agar, Medium 65 and SM3 Agar, were used.
Results: At the end of the isolation process, a total of 179 Streptomyces bacteria were isolated, 145 in Modified Bennett's Agar medium, 8 in SM3 medium and 26 in Medium 65 medium, according to the counting process performed according to the morphological condition after the isolation process.
Conclusion: As a result, it was determined that Streptomyces bacteria isolated from sediment samples taken from fresh water grew mostly in Modified Bennett's Agar medium among Modified Bennett's Agar, SM3 and Medium 65 media. It is thought that Modified Bennett's Agar medium can be used for the identification of new Streptomyces species producing secondary metabolites from aquatic environments

Keywords

Streptomyces, Bennet, Medium 65

Project Number

2017-FDK-5054

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