van vet j Van Veterinary Journal 2149-3359 2149-8644 Van Yuzuncu Yıl University 10.36483/vanvetj.1237613 Veterinary Pharmacology Veteriner Farmakoloji Marbofloksasin, Diklofenak Sodyum ve Metilprednizolonun Sistemik Endotoksemide Serum Sitokin Düzeylerine Etkisi Effect of Marbofloxacin, Diclofenac Sodium and Methylprednisolone on Serum Cytokine Levels in Systemic Endotoxemia https://orcid.org/0000-0001-6614-4347 Öner Ahmet Cihat VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER PR. https://orcid.org/0000-0003-4104-7131 Şahin Ali KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ FAKÜLTESİ 11 27 2023 34 3 189 194 01 18 2023 10 24 2023 Copyright © 1990, Van Veterinary Journal 1990 Van Veterinary Journal

LPS ile deneysel endotoksemi oluşturulan ratlarda marbofloksasin, diklofenak sodyum ve metilprednizolon kullanımının serum sitokin seviyeleri üzerine etkilerinin değerlendirilmesi amaçlanmıştır. Çalışmada 186 adet rat, kontrol grubu (n: 6) ayrıldıktan sonra rastgele 5 eşit gruba ayrıldı. Kontrol grubundan 0. Saat te kan örnekleri alındı. Ratlarda endotoksemi oluşturmak amacı ile intraperitoneal (IP) yolla LPS (4mg/rat) uygulandı. Endotoksemi sonrası gelişen sepsisi tedavi etmek için marbofloksasin 100 mg/kg, diklofenak sodyum 10 mg/kg, metilprednizolon 10 mg/kg dozlarında IP yolla uygulandı. İlaç uygulaması takiben 1, 2, 4, 8, 12 ve 24. saatler de tiyopental anestezisi altında kan örnekleri alınarak serum sitokin değerleri ölçüldü. Araştırmada elde edilen veriler doğal şekillenen sepsise büyük ölçüde benzerlik gösterdi. Sitokin seviyeleri incelendiğinde diklofenak sodyum ile marbofloksasin uygulamasının tek başlarına sepsisi tedavi etmede etkisinin olmadığı, ancak metilprednizolon uygulamasının tek ve kombine yapılması durumunda etkili olabileceği belirlendi. Sepsis ile yükselen sitokin düzeyleri için kortikosteroid uygulamasının tek veya antibiyotik ve NSAİİ’lerle kombine kullanılmasının faydalı olabileceği önerilmektedir.

It was aimed to evaluate the effects of using marbofloxacin, diclofenacsodium and methylprednisolone on serum cytokine levels in rats with experimental endotoxemia with LPS. In the study, 186 rats were randomly divided into 5 equal groups after the control group (n: 6) was separated. Blood samples were taken from the control group at 0 hour. LPS (4mg/rat) was administered intraperitoneally (IP) to induce endotoxemia in rats. To treat sepsis developing after endotoxemia, marbofloxacin 100mg/kg, diclofenacsodium 10 mg/kg, methylprednisolone 10 mg/kg were administered by IP route. Serum cytokine values were measured by taking blood samples under thiopental anesthesia at 1, 2, 4, 8, 12 and 24 hours following drug administration. The data obtained in the study showed a great deal of similarity to naturally occurring sepsis. When cytokine levels were examined, it was determined that the application of diclofenacsodium and marbofloxacin alone did not have an effect in the treatment of sepsis, but methylprednisolone application could be effective in case of single or combined application. It is suggested that corticosteroid administration alone or in combination with antibiotics and NSAIDs may be beneficial for increased cytokine levels with sepsis.

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