Abstract
Goat farming in many countries has significant economic importance. In recent years,thereisa trendtogoat products. Milk quality is associated with somatic cell counts that can be affected intra-mammary infections. The aim of this study is isolation and identification of causative bacterial, yeast and fungal mastitis agents in goat mastitis and detection of antibiotic resistance in bacterial isolates. Totally 220 milk samples from 110 goats in 11 farms were studied for isolation and identification of the agents. And antibacterial resistance of isolated microorganism were studied by disk diffusion method. Totally 30 strains isolated from samples and identification, isolation was made from 28 (12.73%) of 220 milk samples. Among these 30 isolates, 15 (50%) Coagulase Negative Staphylococci, 8 (26.67%) S. aureus, 5 (16.67%) S. uberisand 2 (6.67%) E. coliwas identified. Identification was confirmed by molecular analyses from the direct culture of isolates. The highest resistance were found against to penicillin 21 (70%) and amoxicillin 19 (63.33%), and isolates highly sensitive to gentamicin 30 (100%), enrofloxacin 28 (93.3%), oxytetracycline 28 (93.3%) and amoxicillin + clavulanic acid 27 (90%). Periodical studies and constant monitoring of antibiotic resistance might be beneficial for therapy and prevention strategies and useful management acquisition of antibiotic resistance.
Keywords
References
- Ali Z, Muhammad G, Ahmad T, Khan R, Nazet S (2010). Prevalence of Caprine Sub-Clinical Mastitis, its Etiological Agents and their Sensitivity to Antibiotics in Indigenous Breeds of Kohat, Pakistan. Pak J Life Soc Sci, 8(1), 63–67. Anonym (2016). Hatay ili Tarımsal Yatırım Rehberi, http://www.tarim.gov.tr/SGB/TARYAT/Belgeler/il_yatirim_rehberleri/hatay.pdf Erişim Tarihi: 06 Mart 2016. Aydin İ, Kav K, Çelik HA (2009). Identification and antimicrobial susceptibility of subclinical mastitis pathogens isolated from hair goats’ milk. J Anim Vet Adv, 8, 1086-1090. Bauer AU, Kirby WM, Sherris JC, Tack M (1966). Antibiotic susceptibility testing by a standardized single disc method. J Clin Pathol, 45, 493-494. Bergonier DB, De Crémoux R, Rupp R, Lagriffoul G, Berthelot X (2003). Mastitis of dairy small ruminants. Vet Res, 34, 689–716. Billington SJ, Post KW, Jost BH (2002). Isolation of Arcanobacterium (Actinomyces) pyogenes from cases of feline otitis externa and canine cystitis. J Vet Diagn Invest, 14(2),159-162. Boyazoglu J, Hatziminaoglou I, Morand-Fehr P (2005). The Role of the Goat in Society: Past, Present and Perspectives for the Future. Small Rum Res, 60, 13-23. Cantekin Z, Saidi R, Solmaz H, Ergun Y (2014). A Duplex PCR for Detection of S. aureus and Staphylococcus spp. from Culture and Bovine Milk Samples. YYU Vet Fak Derg, 2014, 25 (1), 11 – 13. Carter GR (1990). Isolation and identification of bacteria from clinical specimens. In: Carter GR, Cole JR. (Eds.) Diagnostic Procedures in Veterinary Bacteriology and Mycology. 5th edition, Academic Press Inc. San Diego, California. Çiftci MK, Berkin Ş, Erer H, Erganiş O, Kıran MM, Hatipoglu F, Saglam YS (1996). Keçi mastitisleri üzerinde patolojik ve bakteriyolojik çalişmalar. Vet Bil Derg, 12(2),105–114. CLSI (2013). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. Wayne, PA: Clinical and Laboratory Standards Institute. Dellal I, Dellal G (2005). Türkiye Keçi Yetiştiriciliğinin Ekonomisi. Süt Keçiciliği Ulusal Kongresi, 26-27 Mayıs, İzmir. Dogruer G, Saribay MK, Aslantas O, Ergun Y, Aslantas O, Demir C, Ates CT (2013). Treatment of subclinical mastitis in Damascus goats during lactation. Small Rum Res 90,153-155. Dogruer G, Saribay MK, Aslantaş Ö, Kireççi E, Ergün Y, Ülkü A, Demir,C (2013). Keçilerde subklinik mastitislerde prevalans, etiyoloji ve mikroorganizmaların antibiyotik duyarlılıkları V.Veteriner Doğum ve Jinekoloji Kongresi, 31 Ekim – 3 Kasım, Antalya, 2013. Foddai A, Idini G, Fusco M, Rosa N, de la Fe C, Zinellu S, Corona L, Tola S (2005). Rapid differential diagnosis of Mycoplasma agalactiae and Mycoplasma bovis based on a multiplex-PCR and a PCR-RFLP. Mol Cell Probes, 19(3), 207-212. Günlü A, Alaşahan S (2010). Türkiye ’de Keçi Yetiştiriciliği ve Geleceği Üzerine Bazı Değerlendirmeler (Evaluations on the Future of Goat Breeding in Turkey). Vet Hekim Der Derg, 81(2), 15–20. IDF (1985). International Dairy Federation, Milk and Milk Products: Guidance on Sampling. International Dairy Federation, Standard 50B, Brussels, Belgium. Ilhan Z, Taşal I, Sagcan S, Solmaz H (2011). Subklinik Mastitisli Keçi Sütlerinden Aerobik Bakterilerin İzolasyonu Isolation of Aerobic Bacteria from Goat Milk with Subclinical Mastitis. YYU Vet Fak Derg, 22(2), 89–91. Islam MA, Samad MA, Anisur Rahman AKM (2011). Bacterial Pathogens and Risk Factors Associated With Mastitis in Black Bengal Goats in Bangladesh. Bangl J Vet Med, 9(2), 155–159. Isnel NB, Kırkan S (2012). Isolation of Microorganisms from Goats with Subclinical Mastitis and Detection of Antibiotics Susceptibility. Subklinik Mastitisli Keçilerden Mikroorganizmaların İzolasyonu ve Antibiyotiklere Duyarlılıklarının Araştırılması. Animal Health Prod and Hyg, 1(2), 106 – 112. Najeeb MF, Anjum AA, Ahmad MUD, Khan HM, Ali MA, Sattar MMK (2013). Bacterial etiology of subclinical mastitis in dairy goats and multiple drug resistance of the isolates. J Anim Plant Sci, 23(6),1541–1544. Niesters HGM, Goessens WHF, Meis JFMG, Quint WGV (1993). Rapid, polymerase chain reaction-based identification assays for Candida species. J Clin Microbiol, 31,904–910. Poutrel B, De Crémoux R, Ducelliez M, Verneau D (1997). Control of intramammary infections in goats: impact on somatic cell counts. J Anim Sci, 75,566-570. Quinn PJ, Carter ME, Markey BK, Carter GR (1994). Clinical Veterinary Microbiology. Mosby-Year Book Europe Limited, Lynton House, London WC1H9LB, England.p. 209-236. Riffon R, Sayasith K, Khalil H, Dubreuil P, Drolet M, Lagace A (2001). Development of a Rapid and Sensitive Test for Identification of Major Pathogens in Bovine Mastitis by PCR. J Clin Microbiol, 39(7), 2584–2589. Sambrook J and Russell W (2001). Molecular cloning: a laboratory manual (3rd ed.), Cold Spring Harbor Press, New York.A8.9-A8.10. 2049-2050. White EC, Hinckley LS (1999). Prevalence of mastitis pathogens in goat milk. Small Rum Res, 33,117-121.
Abstract
Birçok ülkede keçi yetiştiriciliği belirli bir ekonomik öneme sahiptir. Son yıllarda keçi ürünlerine yönelik bir eğilim vardır. Süt kalitesi meme içi infeksiyonlar tarafından etkilenen somatik hücre sayısı ile ilişkilidir. Bu çalışmanın amacı, keçilerde mastitise neden olan bakteri, maya ve mantarların izolasyonu ve identifikasyonu ve ayrıca izole edilen bakterilerin antibiyotik duyarlılıklarının belirlenmesidir. Bu amaçla, 11 farklı keçi çiftliğinde 110 keçiden alınan toplam 220 süt örneğinde etken izolasyonu ve identifikasyonu yapıldı. Ayrıca izole edilen bakteriyel izolatların antibiyotik dirençleri disk diffüzyon testi ile araştırıldı. Çalışmada toplanan 220 adet süt örneğinin 28 (%12.73)’inden 30 adet bakteri izole edildi. Bu 30 izolatın, 15 (%50)’i koagülaz negatif stafilokok, 8 (%26.67)’i S. aureus, 5 (%16.67)’i, S. uberisve 2 (%6.67)’si de E. coliolarak identifiye edildi. İdentifikasyonlar, izolatların direkt kültürlerinden yapılan moleküler analizler ile teyit edildi. Bu suşlarda en yüksek antibiyotik direnci penisilin 21 (%70) ve amoksisilin 19 (%63.33)’e karşı bulundu, buna karşın izolatların 30 (%100)’u gentamisin’e, 28 (%93.3)’i enrofloksasin’e, 28 (%93.3)’i oksitetrasiklin’e ve 27 (%90)’si de amoksisilin + klavulanik asit’e duyarlı bulundu. Yapılacak periyodik çalışmalarla keçi mastitislerinden etkenlerin ve antibiyotik direnç durumlarının belirlenmesi hastalığın tedavi ve korunmasında oldukça yararlı veriler sağlayabilecektir.
Keywords
References
- Ali Z, Muhammad G, Ahmad T, Khan R, Nazet S (2010). Prevalence of Caprine Sub-Clinical Mastitis, its Etiological Agents and their Sensitivity to Antibiotics in Indigenous Breeds of Kohat, Pakistan. Pak J Life Soc Sci, 8(1), 63–67. Anonym (2016). Hatay ili Tarımsal Yatırım Rehberi, http://www.tarim.gov.tr/SGB/TARYAT/Belgeler/il_yatirim_rehberleri/hatay.pdf Erişim Tarihi: 06 Mart 2016. Aydin İ, Kav K, Çelik HA (2009). Identification and antimicrobial susceptibility of subclinical mastitis pathogens isolated from hair goats’ milk. J Anim Vet Adv, 8, 1086-1090. Bauer AU, Kirby WM, Sherris JC, Tack M (1966). Antibiotic susceptibility testing by a standardized single disc method. J Clin Pathol, 45, 493-494. Bergonier DB, De Crémoux R, Rupp R, Lagriffoul G, Berthelot X (2003). Mastitis of dairy small ruminants. Vet Res, 34, 689–716. Billington SJ, Post KW, Jost BH (2002). Isolation of Arcanobacterium (Actinomyces) pyogenes from cases of feline otitis externa and canine cystitis. J Vet Diagn Invest, 14(2),159-162. Boyazoglu J, Hatziminaoglou I, Morand-Fehr P (2005). The Role of the Goat in Society: Past, Present and Perspectives for the Future. Small Rum Res, 60, 13-23. Cantekin Z, Saidi R, Solmaz H, Ergun Y (2014). A Duplex PCR for Detection of S. aureus and Staphylococcus spp. from Culture and Bovine Milk Samples. YYU Vet Fak Derg, 2014, 25 (1), 11 – 13. Carter GR (1990). Isolation and identification of bacteria from clinical specimens. In: Carter GR, Cole JR. (Eds.) Diagnostic Procedures in Veterinary Bacteriology and Mycology. 5th edition, Academic Press Inc. San Diego, California. Çiftci MK, Berkin Ş, Erer H, Erganiş O, Kıran MM, Hatipoglu F, Saglam YS (1996). Keçi mastitisleri üzerinde patolojik ve bakteriyolojik çalişmalar. Vet Bil Derg, 12(2),105–114. CLSI (2013). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. Wayne, PA: Clinical and Laboratory Standards Institute. Dellal I, Dellal G (2005). Türkiye Keçi Yetiştiriciliğinin Ekonomisi. Süt Keçiciliği Ulusal Kongresi, 26-27 Mayıs, İzmir. Dogruer G, Saribay MK, Aslantas O, Ergun Y, Aslantas O, Demir C, Ates CT (2013). Treatment of subclinical mastitis in Damascus goats during lactation. Small Rum Res 90,153-155. Dogruer G, Saribay MK, Aslantaş Ö, Kireççi E, Ergün Y, Ülkü A, Demir,C (2013). Keçilerde subklinik mastitislerde prevalans, etiyoloji ve mikroorganizmaların antibiyotik duyarlılıkları V.Veteriner Doğum ve Jinekoloji Kongresi, 31 Ekim – 3 Kasım, Antalya, 2013. Foddai A, Idini G, Fusco M, Rosa N, de la Fe C, Zinellu S, Corona L, Tola S (2005). Rapid differential diagnosis of Mycoplasma agalactiae and Mycoplasma bovis based on a multiplex-PCR and a PCR-RFLP. Mol Cell Probes, 19(3), 207-212. Günlü A, Alaşahan S (2010). Türkiye ’de Keçi Yetiştiriciliği ve Geleceği Üzerine Bazı Değerlendirmeler (Evaluations on the Future of Goat Breeding in Turkey). Vet Hekim Der Derg, 81(2), 15–20. IDF (1985). International Dairy Federation, Milk and Milk Products: Guidance on Sampling. International Dairy Federation, Standard 50B, Brussels, Belgium. Ilhan Z, Taşal I, Sagcan S, Solmaz H (2011). Subklinik Mastitisli Keçi Sütlerinden Aerobik Bakterilerin İzolasyonu Isolation of Aerobic Bacteria from Goat Milk with Subclinical Mastitis. YYU Vet Fak Derg, 22(2), 89–91. Islam MA, Samad MA, Anisur Rahman AKM (2011). Bacterial Pathogens and Risk Factors Associated With Mastitis in Black Bengal Goats in Bangladesh. Bangl J Vet Med, 9(2), 155–159. Isnel NB, Kırkan S (2012). Isolation of Microorganisms from Goats with Subclinical Mastitis and Detection of Antibiotics Susceptibility. Subklinik Mastitisli Keçilerden Mikroorganizmaların İzolasyonu ve Antibiyotiklere Duyarlılıklarının Araştırılması. Animal Health Prod and Hyg, 1(2), 106 – 112. Najeeb MF, Anjum AA, Ahmad MUD, Khan HM, Ali MA, Sattar MMK (2013). Bacterial etiology of subclinical mastitis in dairy goats and multiple drug resistance of the isolates. J Anim Plant Sci, 23(6),1541–1544. Niesters HGM, Goessens WHF, Meis JFMG, Quint WGV (1993). Rapid, polymerase chain reaction-based identification assays for Candida species. J Clin Microbiol, 31,904–910. Poutrel B, De Crémoux R, Ducelliez M, Verneau D (1997). Control of intramammary infections in goats: impact on somatic cell counts. J Anim Sci, 75,566-570. Quinn PJ, Carter ME, Markey BK, Carter GR (1994). Clinical Veterinary Microbiology. Mosby-Year Book Europe Limited, Lynton House, London WC1H9LB, England.p. 209-236. Riffon R, Sayasith K, Khalil H, Dubreuil P, Drolet M, Lagace A (2001). Development of a Rapid and Sensitive Test for Identification of Major Pathogens in Bovine Mastitis by PCR. J Clin Microbiol, 39(7), 2584–2589. Sambrook J and Russell W (2001). Molecular cloning: a laboratory manual (3rd ed.), Cold Spring Harbor Press, New York.A8.9-A8.10. 2049-2050. White EC, Hinckley LS (1999). Prevalence of mastitis pathogens in goat milk. Small Rum Res, 33,117-121.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | August 20, 2016 |
| Submission Date | February 1, 2016 |
| Acceptance Date | March 28, 2016 |
| Published in Issue | Year 2016 Volume: 27 Issue: 2 |
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