Immunohistochemistry and PCR methods for the Diagnosis of BVDV in Cattle with Pneumonia in Erzurum Region

Year 2016, Volume: 27 Issue: 2, 85 - 89, 20.08.2016

Mustafa Özkaraca Mehmet Özkan Timurkan

Abstract

This study was aimed to present Bovine Viral Diarrhea Virus (BVDV)in bovine pneumonias with PCR and immunohistochemistry in Erzurum region, also aimed to determine what is the prevalence of agent in sampling term (01 December 2015-15 February 2016). For this purpose 600 bovine lungs were investigated in slaughterhouses that operated in Erzurum and surrounding counties. Due to evaluation of 72 (12%) lungs with pneumonia diagnosed macroscopically, the intersititial pneumonia n=43, suppurative bronchopneumonia n=23, and fibrinous pneumonia n=3 were classified according to histopathological findings. Immunpositivities of BVDV antigens were observed in 4 out of 72 (5.5%) samples. Same positive samples were confirmed with pestivirus genus specific primers to differentiate cross binding and dyeing in immunohistochemical procedures. Immunopositivity of the samples were located in bronchial ephitelium, peribronchial, peribronchioler, inflammatory cells in intersitital areas; intima layer of arterioles; lymphoid cells in BALT. As a result we found that BVDV is found in 5.5% of the mature cattle pneumonia cases in Erzurum Region an it is an aetiological agent in natural cases of pneumonia in our sampling time.

Keywords

Cattle, Bovine Viral Diarrhea Virus, Immunohictochemistry, Pneumonia

Erzurum Yöresinde Pnömonili Sığırlarda BVDV’ nin Teşhisinde İmmunohistokimya ve PCR Yöntemleri

Abstract

Bu çalışmada Bovine Viral Diarrhea Virus(BVD)’nin Erzurum yöresi sığır pnömonilerindeki varlığının immunohistokimyasal yöntemle teşhisi ve PCR ile konfirmasyonu ayrıca örneklenen dönem içerisinde ne oranda bulunduğunun tespiti amaçlanmıştır. Bu amaçla ilgili dönem içerisinde (01 Aralık 2015 – 15 Şubat 2016) Erzurum ve çevre ilçelerine hizmet veren bir mezbahada kesime alınan 600 sığır akciğer örneği incelendi. Makroskobik olarak pnömoni bulgusu gösteren 72 (%12) örneğin histopatolojik incelemesinde farklı türlerde pnömoni oldukları (43=intersitisyel pnömoni, 23=suppuratif bronkopnömoni, 3= fibrinöz bronkopnömoni) belirlendi. BVD yönünden immunohistokimyasal yöntemle tespiti yapılan 72 pnömoni örneğinden 4 (%5.5)’ ünde immunpozitiflikler görüldü. Aynı pozitiflikler çapraz bağlanma ve boyanmaları ayırt etmek amacıyla pestivirusgenus spesifik primerler kullanılarak PCR ile de doğrulandı. Bu dokulardaki İmmunpozitifliklere bronş epitel hücrelerinde, peribronşial, peribronşioler, intersitisyel alanlardaki yangısal hücrelerde, arteriollerin intima tabakasında, BALT’taki lenfoid hücrelerde rastlandı. Sonuç olarak; BVD’ nin ilgili dönem içerisinde Erzurum yöresinde erişkin sığır pnömonilerinde %5.5 oranında bulunduğu ve doğal pnömoni olgularında rol oynayan etiyolojik ajanlardan birisi olduğu tespit edilmiştir.

Keywords

Sığır, Bovine Viral Diarrhea Virus, İmmunohistokimya, Pnömoni

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