Determination of Serum Lipid Profile and Lipoprotein Levels of Sheep with Naturally Acute Babesiosis

Year 2017, Volume: 28 Issue: 1, 1 - 4, 20.04.2017

Yuksel Sengul , Handan Mert , Nihat Mert

Abstract

In this study, which was done to determine the
lipid profile and the variations about the levels of lipoprotein in the blood
of sheep naturally infected with Babesia
ovis, 3-5 year old, 30 infected and 15 healthy, totally 45 Morkaraman breed
sheep raised in Erciş county of Van city were used as materials. The
measurement of triglyceride, total cholesterol, LDL and HDL were studied by
using enzymatic colorimetric method. VLDL levels were calculated by formula.
The percentages of free fatty acids, triglyceride, phospholipids, cholesterol
and cholesteryl esters were measured by using thin layer chromatography. In the
infected group it was determined that the levels of triglyceride (P<0.001)
and VLDL (P<0.001) statistically increased, but the HDL (P<0.05) decreased
compared to control group. After the chromatographic determination of lipid
amounts (%), in the infected group it was determined that triglyceride amount
(P<0.001) statistically increased, but phospholipids (p<0.05) and
cholesterol esters amounts (P<0.01) decreased compared to control group. As
a result, it is said to be important that lipid levels in sheep babesiosis and lipoprotein levels are important for
animals in terms of prognosis.

Keywords

Babesia ovis, Cholesterol, HDL, LDL, Triglyceride, VLDL

Doğal Akut Babesiosis’li Koyunlarda Serum Lipit Profili ve Lipoprotein Düzeylerinin İncelenmesi

Abstract

Babesia
ovis ile doğal enfekte olan koyunlarda kandaki lipit profilini ve
lipoprotein düzeylerindeki değişimi saptamak için yapılan bu çalışmada canlı
materyal olarak Van ili Erciş ilçesinde yetiştirilen 3-5 yaşları arasındaki,
toplam 45 Morkaraman ırkı koyun kullanıldı (30 enfekte ve 15 sağlıklı koyun).
Serum örneklerinde trigliserit, total kolesterol, LDL ve HDL ölçümü enzimatik
kolorimetrik yöntem kullanılarak çalışıldı. VLDL düzeyleri formülle hesaplandı.
Serbest yağ asitleri, trigliserit, fosfolipit, kolesterol ve kolesterol esterlerinin
% miktarları ise ince tabaka kromatografisi kullanılarak ölçüldü. Enfekte
hayvanlarda sağlıklı kontrollere göre trigliserit (P<0.001) ve VLDL
düzeylerinin (P<0.001) istatistik olarak önemle arttığı, HDL düzeyinin
(P<0.05) ise azaldığı tespit edildi. Lipit miktarlarının (%) kromatografik
olarak incelenmesi sonucunda enfekte hayvanlarda sağlıklı kontrollere göre
trigliserit miktarının (P<0.001) istatistik olarak önemle arttığı,
fosfolipit (P<0.05) ve kolesterol esterlerinin miktarının (P<0.01) ise
azaldığı saptandı. Sonuç olarak, koyun babesiosisinde lipit profilinin ve
lipoprotein düzeylerinin bilinmesinin hayvanlarda prognoz açısından önemli
olduğu söylenebilir.

Keywords

Babesia ovis, Kolesterol, HDL, LDL, Trigliserit, VLDL

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