The Investigation and Phylogenetic Analysis of Canine Parvovirus 2 Infection from Blood and Rectal Swab Samples from Dogs in Van Province, Turkey

Year 2018, Volume: 29 Issue: 2, 83 - 86, 20.09.2018

Zeynep Karapınar Ender Dinçer Cumali Özkan

Abstract

In this study, it was aimed to determine the presence
of CPV infection in the blood and rectal swaps of suspected dogs that they were
handed to Yuzuncu Yıl University, Faculty of Veterinary, Department of Internal
Disease using PCR techniques and molecular characterization of local viruses.
57 (75%) of the 76 rectal swabs used in the study and 40 (64%) of the 62 blood
samples were determined to be positive by PCR obtained from dogs with clinical
symptoms.
Selected samples were positive with the virus carried the molecular
characterization. The results of the sequence analysis were similar
among themselves and they were found in CPV-2a and CPV-2b groups. It was
determined that the sequences of virus strains showed 99-100% homology with
other strains obtained from Genbank.

Keywords

CPV, PCR, Molecular characterization, Phylogenetic analysis

Türkiyede Van Yöresinde Bulunan Köpeklerden Sağlanan Kan ve Rektal Sıvap Örneklerinde Canine Parvovirus-2 Enfeksiyonunun Araştırılması ve Filogenetik Analizi

Abstract

Bu çalışmada, Van ili Yüzüncü Yıl Üniversitesi
Veteriner Fakültesi İç Hastalıkları Kliniğine getirilen Canine Parvovirus (CPV) Enfeksiyonu şüpheli köpeklerden alınan kan ve
rektal sıvap örneklerinde PZR tekniği kullanılarak CPV enfeksiyonunun
varlığının belirlenmesi ve yerel virusların moleküler karakterizasyonunun
yapılması amaçlandı. Klinik olarak enfeksiyon belirtisi gösteren köpeklerden
sağlanan 76 adet rektal sıvaptan 57 adedi (%75), 62 adet kan örneğinin ise 40
adedi (%64) PZR ile pozitif olarak belirlendi. Seçilen pozitif örnekler
ile virusun moleküler karakterizasyonu gerçekleştirildi. Elde edilen sekans
sonucu bilgileri değerlendirildiğinde örneklerin CPV-2a ve CPV-2b grublarında
bulunduğu ve kendi aralarında benzerlik gösterdikleri belirlendi. Elde edilen virus suşu sekanslarının Genbankasından sağlanan diğer
suşlarla %99-100 oranında homoloji gösterdiği belirlendi.

Keywords

CPV, PZR, Moleküler karakterizasyon, Filogenetik analiz

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