Erkek Sıçanlarda Talyum (I) Sülfatın Neden Olduğu Üreme Hasarına Karşı Vitis vinifera L. cv “Merlot” Tohum Ekstraktının Etkileri

Abstract

Bu çalışmanın amacı sıçanlarda talyum sülfatın neden olduğu üreme hasarına karşı Vitis vinifera L. tohum ekstraktının etkilerini belirlemektir. Bu amaçla çalışmada her grupta 6 adet olmak üzere toplam 24 adet erkek sıçan kullanıldı. Kontrol grubundaki sıçanlara %0,5 karboksimetil selüloz ve ardından serumm fizyolojik verildi. Talyum sülfat grubundaki sıçanlara intraperitoneal enjeksiyon yoluyla talyum sülfat (10 mg/kg) verildi. Talyum sülfat + Vitis vinifera L. ekstresi gruplarında sıçanlara talyum sülfat (10 mg/kg /intr aperi tonea l)+Vi tis vinifera L. ekstraktı (100 mg/kg) peros uygulama yapıldı. Vitis vinifera L. ekstresi grubundaki sıçanlara Vitis vinifera L. ekstresi (100 mg/ kg) peros verildi. Sonra deney süreci tamamlanarak sıçanlar sakrifiye edildi ve kan ve testis dokuları alındı. Çalışmamızın sonuçlarına göre kan ve testislerdeki malondialdehit düzeyinin Talyum sülfat grubunda diğer deney gruplarına göre istatistiksel olarak daha yüksekti (P < .05). Talyum sülfat grubunda katalaz, glutatyon peroksidaz aktivitesi ve glutatyon düzeyi en düşük seviyedeydi(P < .05). Histopatolojik incelemede talyum sülfat grubunda nekrotik ve dejeneratif bulgular görülmektedir. Talyum sülfat grubunda kaspaz-3 güçlü bir şekilde ifade edilirken talyum sülfat+- Vitis vinifera L. ekstrakt grubunda kaspaz-3 seviyesi inhibe edildi. Talyum sülfat grubunda total motilite değerinin diğer deney grupları ile karşılaştırıldığında daha düşük olduğu görüldü (P < .05). Sonuç olarak sıçanlarda Talyum sülfat tarafından bozulan fizyolojik fonksiyonlara karşı peros olarak uygulanan Vitis vinifera L. ekstraktı’nın biyokimyasal, histopatolojik ve spermatolojik iyileştirici etkileri olduğu belirlendi.

Keywords

• Kaspaz 3
• oksidatif stres
• testis
• talyum
• Vitis vinifera L

Effects of Vitis vinifera L. cv. “Merlot” Seed Extract Against Thallium (I) Sulfate-Induced Reproductive Damage in Male Rats

Abstract

The aim of the present study was to determine the effects of Vitis vinifera L. seed extract against reproductive damage induced by thallium sulfate in rats. For this purpose, a total of 24 male rats, 6 rats in each group, were used for the study. In the control group, the rats were given 0.5% carboxymethyl cellulose followed by physiological saline. In the thallium sulfate group, the rats were given thallium sulfate (10 mg/kg) by intraperitoneal injection. In thallium sulfate + Vitis vinifera L. extract groups,The rats were administered thallium sulfate (10 mg/kg/intraperitoneal) + Vitis vinifera L. extract (100 mg/kg) peros. In Vitis vinifera L. extract group, the rats were given Vitis vinifera L. extract (100 mg/kg) peros. After the experimental process was finished, the rats were sacrificed and the blood and testicular tissues were taken. The results of our study found that the malondialdehyde level in the blood and testicular tissues of the thallium sulfate group was statistically higher than the other experimental groups (P < .05). Catalase, glutathione peroxidase activity, and glutathione level were the lowest in the thallium sulfate group (P < .05). In the histopathological examination, necrotic and degenerative findings are seen in the thallium sulfate group. Cleaved caspase-3 was strongly expressed in seminiferous tubules in the thallium sulfate group, but cleaved caspase-3 level was inhibited in thallium sulfate + Vitis vinifera L. extract group. Total motility value was found to be lower in the thallium sulfate group compared to the other experimental groups (P < .05). As a result, the biochemical, histopathological, and spermatological effects of Vitis vinifera L. extract administered as a peros against the deteriorated physiological functions in rats given thallium sulfate were determined.

Keywords

• Cleaved caspase 3
• oxidative stress
• testis
• thallium
• Vitis vinifera L

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