yyu jinas Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 1300-5413 2667-467X Van Yüzüncü Yıl Üniversitesi 10.53433/yyufbed.1077700 Engineering Mühendislik Peynir Altı Suyu Proteini Konsantresinin Partikül Boyutu ve Emülsifiye Edici Özellikleri Üzerine Ultrason Sürelerinin ve Genliklerinin Etkileri The Effects of Ultrasound Times and Amplitudes on the Particle Size and Emulsifying Properties of Whey Protein Concentrate https://orcid.org/0000-0003-3902-6403 Bulut Menekşe IĞDIR ÜNİVERSİTESİ 08 30 2022 27 2 323 329 02 23 2022 05 29 2022 Copyright © 1995, Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences 1995 Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences

Mevcut çalışma, peynir altı suyu proteini konsantresinin (PASPK) partikül boyutları ve emülsifiye edici özellikleri üzerindeki ultrason sürelerinin ve genliklerinin etkisini araştırmak için yapılmıştır. Ultrason (US) uygulaması, 20 kHz frekans ile farklı zamanlarda (10, 20 ve 30 dakika %50 genlikte) ve amplitüdlerde (5 dakika için %60, %80 ve %100) VC-7500 ultrasonik güç ekipmanı kullanılarak yapıldı. Sonuçlar, US işleminin hem partikül boyutları hem de emülsifiye edici özellikler üzerinde önemli (p<0.05) bir etkiye sahip olduğunu gösterdi. En küçük parçacık boyutu, %100 genlikte (US310) (498.6 nm) 30 dakika (dak.) US'ye maruz bırakılan PASPK numunelerinde belirlendi. %100 genlikte işlenen PASPK numuneleri, %60 ve %80 genliklerde diğer PASPK numunelerine kıyasla daha küçük parçacık boyutu gösterdi. US ile 10 dak. muamele edilen PASPK numunelerinin partikül boyutu en büyük (790.3 nm) düzeyde iken, 30 dak. süre ile muamele edilen PASPK numunelerinde en küçük partikül boyutu (697.1 nm) tespit edilmiştir. Öte yandan, işlemler arasında US310 PASPK numuneleri en yüksek emülsiyon aktivitesi indeksine (EAİ) (198 m2/g) ve emülsiyon stabilitesi indeksine ( ESİ) (34.0 dak.) sahipken, işlem görmemiş PASK numunelerinde en düşük EAİ (56 m2/g) ve ESİ (13.0 dak.) saptandı. Genel olarak, %100 genlikte 30 dakikalık US uygulaması, diğer sonikasyon süreleri ve amplitüdleriyle karşılaştırıldığında en düşük parçacık boyutu (498.6 nm) ve en yüksek emülsiyon özellikleri (EAİ:198 m2/g ve ESİ: 34.0) gösterdi.

The current study was conducted to investigate the effects of ultrasound times and amplitudes on the particle sizes and emulsifying properties of samples of whey protein concentrate (WPC). The ultrasound (US) application was performed using VC-7500 ultrasonic power equipment at a 20 kHz frequency, at various times (10, 20, and 30 min at a 50% amplitude) and amplitudes (60%, 80%, and 100% for 5 min). The results showed that the US procedure had a significant effect (p<0.05) on both particle sizes and emulsifying properties (p<0.05). The smallest particle size was obtained for the WPC samples exposed to 30 min of US at a 100% amplitude (US310) (498.6 nm). The WPC samples treated at a 100% amplitude showed a smaller particle size compared to the other WPC samples at 60% and 80% amplitudes. While the WPC samples treated for 10 min had the biggest particle size (790.3 nm), those treated for 30 min had the smallest particle size (697.1 nm). On the other hand, among the treatments, US310 whey protein concentrate samples had the highest EAI (emulsifying activity index) (198 m2/g) and ESI (emulsion stability index) (34.0 min), whereas the untreated WPC samples had the lowest EAI (56 m2/g) and ESI (13.0 min). In general, 30-min US treatment at a 100% amplitude showed the lowest particle size (498.6 nm) and the highest emulsifying properties (EAI: 198 m2/g and ESI: 34.0) compared to the other sonication times and amplitudes.

 Amplitude Emulsifying properties Particle size Ultrasound Whey protein concentrate Emülsifiye edici özellikler Genlik Parçacık boyutu Peynir altı suyu proteini konsantresi Ultrason Abd El-Salam, M. H., El-Shibiny, S., & Salem, A. (2009). Factors affecting the functional properties of whey protein products: A review. Food Reviews International, 25(3), 251-270. doi:10.1080/87559120902956224 Arzeni, C., Martinez, K., Zema, P., Arias, A., Perez, O. E., & Pilosof, A. M. R. (2012). Comparative study of high intensity ultrasound effects on food proteins functionality. Journal of Food Engineering, 108, 463-472. doi:10.1016/j.jfoodeng.2011.08.018 Gordon, L., & Pilosof, A. M. (2010). Application of high-intensity ultrasounds to control the size of whey proteins particles. Food Biophysics, 5(3), 203-210. doi: 10.1007/s11483-010-9161-4 Jambrak, A. R., Mason, T. M., Lelas, V., Herceg, Z., & Herceg, I. L. (2008). Effect of ultrasound treatment on solubility & foaming properties of whey protein suspensions. Journal of Food Engineering, 86, 281–287. doi:10.1016/j.jfoodeng.2007.10.004 Jambrak, A. R., Mason, T. J., Lelas, V., Paniwnyk, L., & Herceg, Z. (2014). Effect of ultrasound treatment on particle size & molecular weight of whey proteins. Journal of Food Engineering, 121, 15-23. doi:10.1016/j.jfoodeng.2013.08.012 Jiang, S., Yıldız, G., Ding, J., Andrade, J., Rababah, T. M., Almajwalc, A., Abulmeatyc, M. M., & Feng, H. (2019). Pea protein nanoemulsion and nanocomplex as carriers for protection of cholecalciferol (vitamin D3). Food and Bioprocess Technology, 12(6), 1031-1040. doi:10.1007/s11947-019-02276-0 Karki, B., Lamsal, B. P., Jung, S., van Leeuwen, J. H., Pometto III, A. L., Grewell, D., & Khanal, S. K. (2010). Enhancing protein and sugar release from defatted soy flakes using ultrasound technology. Journal of Food Engineering, 96(2), 270-278. doi:10.1016/j.jfoodeng.2009.07.023 Krešić, G., Lelas, V., Jambrak, A. R., Herceg, Z., & Brnčić, S. R. (2008). Influence of novel food processing technologies on the rheological & thermophysical properties of whey proteins. Journal of Food Engineering, 87(1), 64–73. doi:10.1016%2Fj.jfoodeng.2007.10.024 Kumar, R., Chauhan, S. K., Shinde, G., Subramanian, V., & Nadanasabapathi, S. (2018). Whey Proteins: A potential ingredient for food industry- A review. Asian Journal of Dairy and Food Research, 37, 283-290. doi:10.18805/ajdfr.DR-1389 Lam, R. (2014). Tailoring of whey protein isoalte stabilized oil-water interfaces for improved emulsification. (PhD), Saskatchewan University, Department of Food and Bioproduct Sciences Saskatoon, SK. Lee, H., Yildiz, G., Dos Santos, L. C., Jiang, S., Andrade, J. E., Engeseth, N. J., & Feng, H. (2016). Soy protein nano-aggregates with improved functional properties prepared by sequential pH treatment and ultrasonication. Food Hydrocolloids, 55, 200-209. doi:10.1016/j.foodhyd.2015.11.022 Liu, Q., Li, J., Kong, B. H., Li, P. J., & Xia, X. F. (2014). Physicochemical & antioxidant properties of Maillard reaction products formed by heating whey protein isolate & reducing sugars. International Journal of Dairy Technology, 67, 220-228. doi:10.1111/1471-0307.12110 Mason, T. J., Paniwnyk, L., & Lorimer, J. P. (1996). The uses of ultrasound in food technology. Ultrasonics Sonochemistry, 3, 253–260. doi:10.1016/S1350-4177(96)00034-X Vargas, S. A., Delgado-Macuil, R. J., Ruiz-Espinosa, H., Rojas-López, M., & Amador-Espejo, G. G. (2021). High-intensity ultrasound pretreatment influence on whey protein isolate and its use on complex coacervation with kappa carrageenan: Evaluation of selected functional properties. Ultrasonics Sonochemistry, 70, 105340. doi:10.1016/j.ultsonch.2020.105340 Yanjun, S., Jianhang, C., Shuwen, Z., Hongjuan, L., Jing, L., Lu, L., Uluko, H., Yanling, S., Wenming, C., Wupeng, G., & Jiaping, L. (2014). Effect of power ultrasound pre-treatment on the physical and functional properties of reconstituted milk protein concentrate. Journal of Food Engineering, 124, 11-18. doi:10.1016/j.jfoodeng.2013.09.013 Yildiz, G., Andrade, J., Engeseth, N. E., & Feng, H. (2017). Functionalizing soy protein nano-aggregates with pH-shifting & mano-thermo-sonication. Journal of Colloid and Interface Science, 505, 836-846. doi:10.1016/j.jcis.2017.06.088 Yıldız, G. (2018). Physicochemical properties of soy protein concentrate treated with ultrasound at various amplitudes. Journal of the Institute of Science and Technology, 8(4), 133-139. doi:10.21597/jist.436852 Yıldız, G. (2019). Application of ultrasound & high pressure homogenization against high temperature-short time in peach juice. Journal of Food Process Engineering, 42(3), e12997. doi:10.1111/jfpe.12997 Yildiz, G., & Feng, H. (2019). Sonication of cherry juice: comparison of different sonication times on color, antioxidant activity, total phenolic and ascorbic acid content. Latin American Applied Research-An International Journal, 49(4), 255-260. Yıldız, G., & Aadil, R. M. (2020). Comparison of high temperature-short time & sonication on selected parameters of strawberry juice during room temperature storage. Journal of Food Science and Technology, 57(4), 1462-1468. doi:10.1007/s13197-019-04181-y