yyu jinas Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 1300-5413 2667-467X Van Yuzuncu Yıl University 10.53433/yyufbed.1452862 Phytopathology Fitopatoloji Absisik Asit ve Arbusküler Mikorizal Fungus Uygulamalarının Fasulyede Kömür Çürüklüğü Hastalığı [(Macrophomina phaseolina) (Tassi) Goid.] ve Bitki Gelişimine Etkisi Abscisic Effect of Abscisic Acid and Arbuscular Mycorrhizal Fungi Applications on Coal Rot Disease [(Macrophomina phaseolina) (Tassi) Goid.] and Plant Growth in Bean https://orcid.org/0009-0006-9053-9529 Vural Ali DSİ https://orcid.org/0000-0002-0177-7677 Demir Semra Van Yüzüncü Yıl Üniversitesi https://orcid.org/0000-0003-1208-032X Erdinç Çeknas VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ 08 31 2024 29 2 745 760 03 19 2024 05 16 2024 Copyright © 1995, Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences 1995 Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences

Bu çalışmada, fasulyede önemli bir patojen olan kömür çürüklüğü hastalığı etmenine karşı (Macrophomina phaseolina (Tassi) (Goidanich) (Mp) Arbusküler mikorizal fungus (AMF) ve absisik asit (ABA) uygulamasının etkileri incelenmiştir. İlk aşamada in vitro koşullarda ABA’nın 0, 1, 5, 10, 20 μM dozlarının Mp’ya karşı etkisine bakılmış ve 5 μM ABA dozunun Mp’nın koloni gelişimini yüksek oranda kısıtladığı görülmüştür. İkinci aşamada, iklim odası koşullarında yetiştirilen Gina fasulye çeşidine ait fidelerde AMF ve farklı ABA dozlarının bitki gelişimi ve kömür çürüklüğü hastalığına etkileri incelenmiştir. Bazı fide gelişim parametrelerinde (yaprak sayısı, toplam yaprak alanı, yaş ağırlık, kuru ağırlık) tüm ABA dozları kontrole göre azalmaya neden olurken, bazı parametrelerde ise (toplam yaprak alanı, yaş ve kuru ağırlık) 5 µM ABA’nın diğerlerine göre daha etkili olduğu bulunmuştur. Tek başına ABA dozlarının hastalık şiddeti üzerindeki etkisinde, 5 μM ABA dışındaki diğer dozların hastalık şiddetini arttırdığı belirlenmiştir. Genel olarak ABA dozlarının AMF ile birlikte uygulanmasının %33 oranında hastalık şiddetinde azalmaya neden olduğu saptanmıştır. Elde edilen sonuçlar, uygun ABA dozu ve AMF’nin kombine uygulanmasının hastalık yönetiminde etkili olabileceğini göstermiştir.

In this study, the effects of arbuscular mycorrhizal fungi (AMF) and abscisic acid (ABA) treatment against charcoal rot disease, an important pathogen of bean (Macrophomina phaseolina (Tassi) (Goidanich) (Mp) were investigated. In the first stage, the effects of 0, 1, 5, 10, 20 μM doses of ABA against Mp were investigated under in vitro conditions and it was observed that 5 μM ABA dose highly restricted the colony growth of Mp. In the second stage, the effects of AMF and different ABA doses on plant growth and charcoal rot disease in seedlings of Gina bean cultivar grown under climate chamber conditions were investigated. While all ABA doses caused a decrease in some seedling growth parameters (number of leaves, total leaf area, wet weight, dry weight) compared to the control, in some parameters (total leaf area, wet and dry weight) 5 µM ABA was found to be more effective than the others. In the effect of ABA doses alone on disease severity, it was determined that all doses except 5 μM ABA increased the disease severity. In general, it was determined that the application of ABA doses together with AMF caused a 33% decrease in disease severity. The results obtained showed that the combined application of appropriate ABA dose and AMF may be effective in disease management.

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