Antimicrobial, Antibiofilm and Antiurease Activities of Microbially Synthesized Silver Nanoparticles against Proteus mirabilis

Year 2023, Volume: 28 Issue: 2, 359 - 369, 31.08.2023

Sümeyra Gürkök , Murat Özdal

https://doi.org/10.53433/yyufbed.1194875

Abstract

Nanoparticles (NPs) are tiny materials ranging in size from 1 to 100 nm and have unique magnetic, electrical, and optical characteristics differing from bulk materials. They have a broad spectrum of applications in different industries. Several physical and chemical techniques have been applied to produce metal NPs. Alternatively, green synthesis offers an environmentally friendly and simple means for NP preparation. In the present study, silver NPs were produced by the Pseudomonas aeruginosa OG1 strain. Characterization of NPs was performed by TEM, SEM, and XRD. These NPs were used against pathogenic Proteus mirabilis, which shows high-level urease activity and forms clear biofilms. Silver NPs obtained in the present study were applied to inhibit the growth, urease production, and biofilm formation of P. mirabilis. Growth inhibition zones of 9 mm and 11 mm and, 60 % and 85% antibiofilm effects were obtained by 100 µg mL-1 and 200 µg mL-1 NPs, respectively. The urease activity of P. mirabilis was completely inhibited in both concentrations. These results show that AgNPs can be used as effective antimicrobial, antibiofilm, and antiurease agents in the fight against pathogens.

Keywords

Antibiofilm, Antimicrobial, Antiurease, Nanoparticles, P. aeruginosa Proteus mirabilis, Silver

Supporting Institution

Atatürk University

Project Number

No grant number

Thanks

This research was supported by Ataturk University (No grant number).

Mikrobiyal Olarak Sentezlenen Gümüş Nanopartiküllerin Proteus mirabilis'e Karşı Antimikrobiyal, Antibiyofilm ve Antiüreaz Aktiviteleri

Abstract

Nanopartiküller (NP), 1 ila 100 nm arasında değişen küçük malzemelerdir ve bulk malzemelerden farklı benzersiz manyetik, elektriksel, optik özelliklere sahiptir. Farklı endüstrilerde geniş bir uygulama alanlarına sahiptirler. Metal NP'leri üretmek için çeşitli fiziksel ve kimyasal teknikler uygulanmıştır. Alternatif olarak, yeşil sentez, NP hazırlama için çevre dostu ve basit bir yol sunar. Bu çalışmada, gümüş NP'ler Pseudomonas aeruginosa OG1 suşu tarafından üretilmiştir. NP'lerin karakterizasyonu TEM, SEM ve XRD ile yapılmıştır. Bu NP'ler, yüksek düzeyde üreaz aktivitesi gösteren ve berrak biyofilmler oluşturan patojenik Proteus mirabilis'e karşı kullanılmıştır. Bu çalışmada elde edilen gümüş NP'ler P. mirabilis'in büyümesini, üreaz üretimini ve biyofilm oluşumunu engellemek için uygulanmıştır. Sırasıyla 100 µg mL-1 ve 200 µg mL-1 konsantrasyonlardaki NP'lerle 9 mm ve 11 mm'lik büyüme inhibisyon bölgeleri ve %60 ve %85 antibiyofilm etkileri elde edildi. P. mirabilis'in üreaz aktivitesi her iki konsantrasyonda da tamamen inhibe edilmiştir. Bu sonuçlar, AgNP'lerin patojenlerle mücadelede etkili antimikrobiyal, antibiyofilm ve antiüreaz ajanları olarak kullanılabileceğini göstermektedir.

Keywords

Antibiyofilm, Antimikrobiyal, Antiüreaz, Gümüş, Nanopartiküller, P. aeruginosa Proteus mirabilis

Project Number

No grant number

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