Türkiye’nin Iğdır İli’nde sararma ve çalımsı görünüm gösteren biber bitkilerinde ‘Candidatus phytoplasma trifolii’nin varlığı

Year 2022, Volume: 59 Issue: 3, 429 - 437, 30.09.2022

Abdullah Güller , Mustafa Usta , Gülüstan Korkmaz , Serap Demirel , Zeynelabidin Kurt

https://doi.org/10.20289/zfdergi.1081067

Abstract

Amaç: Bu çalışmanın amacı fitoplazma belirtisi gösteren biberlerde fitoplazma etmeninin grup/alt grubunu araştırmaktır.
Materyal ve Yöntem: Bu çalışmada, 2020 yılında Iğdır ilinden toplanan bitkiler direct ve nested PCR testleri kullanılarak analiz edilmiş ve BLASTn, iphyClassifier, Mega 7 ve pDRAW32 programları kullanılmıştır.
Araştırma Bulguları: Testlerde, fitoplazmaya spesifik yaklaşık 1.2 kb DNA fragmentleri elde edilmiştir. Belirlenen 16S rRNA nükleotit dizisi (1254 bp) (OM663745) %99.44'ten fazla diğer ‘Ca. P. trifolii’ üyelerine nükleotit benzerliği göstermiştir. Gerçekleştirilen Sanal RFLP ve filogenetik analizler simptom gösteren biber bitkilerinde Clover proliferation group (16SrVI) grubu ve A alt grubundaki ‘Ca. P. trifolii’ etmeninin infeksiyonunu kanıtlamıştır.
Sonuç: Türkiye’nin Iğdır ilinde doğal olarak enfekte olmuş biberlerde ‘Ca. P. trifolii’ varlığı PCR-RFLP ve kladistik analiz kullanılarak tespit edilmiştir.

Keywords

: Clover proliferation group (16SrVI), Nested PCR, Capsicum annuum, RFLP

Supporting Institution

Bulunmamaktadır

Project Number

-

Thanks

Dergi Editörü ve sekretaryasına teşekkür ederim. Çalışmada emeği geçen tüm arkadaşlarıma teşekkür ederim.

Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey

Abstract

Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms.
Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used.
Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other ‘Ca. P. trifolii’ members. The tentative RFLP and phylogenetic analyzes performed proved the ‘Ca. P. trifolii’ the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants.
Conclusion: The presence of ‘Ca. P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.

Keywords

Clover proliferation group (16SrVI), Nested PCR, Capsicum annuum, RFLP

Project Number

-

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