Determination of Allelic Variation of Karahan-99 × BW3 Bread Wheat Population F4 Individuals Using DNA Markers

Year 2023, Issue: 378, 58 - 66, 30.12.2023

İlker Yüce , Münire Topsakal , Ali Tekin , İmren Çöken Tekin , Ayşe Nur Demirezen , Ali Şenay , Burakhan Korucu , Ali Korkmaz , Hüseyin Güngör , İlker Aydoğdu , Ziya Dumlupınar

https://doi.org/10.33724/zm.1373298

Abstract

Wheat is the main nutrient that mankind has used since settled life. Plant breeders are making great efforts to increase the yield of wheat plant in parallel with the increasing world population. In this study, 22 F4 lines obtained as a result of crossing Karahan-99 bread wheat variety with BW3 wheat genotypes were characterized with 11 functional DNA markers belong to some agronomic traits.
Of the 11 markers used in the study, seven worked (Xgwm18, Xwgp118, Xgwm131, Xgwm47, Sun479, Sun209 and UHW89) in Karahan-99 × BW3 Bread Wheat Population. While the highest number of alleles was obtained from the Sun479 marker (18 bands), the lowest band number was obtained from the markers UHW89 and Xgwmn131 (1 band). The mean number of alleles was 6.71, while the mean polymorphism information content (PIC) was 0.99. Xgwm131 marker encoding Yr39 yellow rust allele was interrogated at Karahan-99_M6_72-10/BW3_F4_3 genotype, while Xgwm18 marker amplified Yr26 stripe rust resistant gene at Karahan-99_M6_72-10/BW3_F4_22.

Keywords

Bread wheat, Molecular marker, Yellow rust

Project Number

2020/4-6 M (YLS)

Karahan-99 × BW3 Ekmeklik Buğday Popülasyonu F4 Bireylerinin Allelik Varyasyonunun DNA Markörleri ile Belirlenmesi

Abstract

Buğday insanoğlunun yerleşik hayata geçtiğinden beri en çok kullandığı temel besin maddesidir. Bitki ıslahçıları, artan Dünya nüfusuna paralel olarak buğday bitkisinde de verimi artırmak için çok büyük gayretler ortaya koymaktadırlar. Bu çalışmada Karahan-99 ekmeklik buğday çeşidi ile BW3 buğday genotipinin melezlenmesi sonucu elde edilen 22 F4 hattı bazı özellikler ile ilişkili olduğu bilinen 11 fonksiyonel DNA markörü ile karakterize edilmiştir.
Araştırmada kullanılan 11 markörden 7 tanesi (Xgwm18, Xwgp118, Xgwm131, Xgwm47, Sun479, Sun209 ve UHW89) Karahan-99 × BW3 ekmeklik buğday popülasyonunda polimorfik bant üretmiştir. En fazla allel sayısı Sun479 marköründen (18 adet) elde edilirken, en az bant sayısı da UHW89 ve Xgwm131 (1 adet) markörlerinden elde edilmiştir. Ortalama allel sayısı 6.71 olurken, ortalama polimorfizm bilgi içeriği 0.99 olarak tespit edilmiştir. Xgwm131 markörü Karahan-99_M6_72-10/BW3_F4_10 genotipinde Yr39 sarı pas geni ile ilişkili allele sahip olurken, Xgwm18 markörü Karahan-99_M6_72-10/BW3_F4_22 genotipinde Yr26 sarı pas dayanıklılık geni ile ilişkili beklenen uzunlukta bant vermiştir.

Keywords

Ekmeklik buğday, Moleküler markör, Sarı pas

Supporting Institution

KSÜ Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2020/4-6 M (YLS)

Thanks

Bu çalışma, KSÜ Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiş olup proje numarası 2020/4-6 M (YLS)’dir. Bu projeyi finansal olarak destekleyen KSÜ BAP Birimine teşekkürlerimizi sunarız.

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