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Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study

Yıl 2017, Cilt: 7 Sayı: 2, 57 - 61, 30.09.2017

Öz

Objective: The aim of the present pilot study was to evaluate the effects of a single local dose administration of humic acid on healing of subcondylar mandibular fractures in rats.
Methods: In this study, a randomized experimental protocol was used. The study was conducted with 16 male Wistar-albino rats that were 16–18 weeks old. The rats (n=16) were randomly divided into two groups: Group HA received humic acid (0.3 cc/site, n=8) and Group C received no additional medical administration (control group, n=8). A full-thickness surgical osteotomy was performed in the subcondylar area. A single dose of humic acid (0.3 cc/site) was administered locally by spraying on the bone surfaces of the fracture line. Mandible was dissected on postoperative day 21. Then, fractured hemimandibles were obtained for histopathological examination.
Results: The median score of bone fracture healing was 7.16 (range: 7 to 8) in the Group HA and 7.50 (range: 7 to 8) in the Group C. When the groups were compared in terms of bone healing scores, there was no statistical difference between the Group HA and the Group C (p>0.05).
Conclusion: Results of this study showed that local administration of humic acid was not efficient for healing of bone fractures. However, we are of the opinion that it is required to conduct more comprehensive studies, including humic acid’s different concentrations and administration manners, evaluating the effects of humic acid on tissue both histopathologically
and in terms of inflammatory and proinflammatory cytokine levels.

Kaynakça

  • 1. Schindeler A, McDonald MM, Bokko P, Little DG. Bone remodeling during fracture repair: the cellular picture. Semin Cell Dev Biol 2008;19:459–66. 2. Dimitriou R, Jones E, McGonagle D, Giannoudis PV. Bone regeneration: current concepts and future directions. BMC Med 2011:31;9:66. 3. Fischer C, Doll J, Tanner M, et al. Quantification of TGF-ß1, PDGF and IGF-1 cytokineexpression after fracture treatment vs. non-union therapy via masquelet. Injury 2016;47:342–9. 4. Tsiridis E, Upadhyay N, Giannoudis P. Molecular aspects of fracture healing: which are the important molecules? Injury 2007;38(Suppl 1):S11–25. 5. Moghaddam A, Müller U, Roth HJ, Wentzensen A, Grützner PA, Zimmermann G. TRACP 5b and CTX as osteological markers of delayed fracture healing. Injury 2011; 42:758–64. 6. Smith DD, Gowen M, Mundy GR. Effects of interferon-gamma and other cytokines on collagen synthesis in fetal rat bone cultures. Endocrinology 1987;120:2494–9. 7. Yang S, Ding W, Feng D, Gong H, Zhu D, Chen B, Chen J. Loss of B cell regulatory function is associated with delayed healing in patients with tibia fracture. APMIS 2015;123:975–85. 8. Asagiri M, Takayanagi H. The molecular understanding of osteoclast differentiation. Bone 2007;40:251–64. 9. Islam KMS, Schuhmacher A, Gropp JM. Humic acid substances in animal agriculture. Pakistan Journal of Nutrition 2005;4:126– 34. 10. Hu CW, Yen CC, Huang YL, Pan CH, Lu FJ, Chao MR. Oxidatively damaged DNA induced by humic acid and arsenic in maternal and neonatal mice. Chemosphere 2010;79:93–9. 11. Çal›fl›r M, Akp›nar A, Poyraz Ö, Göze F, Ç›nar Z. The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature-induced periodontitis in rats. J Periodontal Res 2016;51:499–507. 12. Jooné GK, Dekker J, van Rensburg CE. Investigation of the immunostimulatory properties of oxihumate. Z Naturforsch C 2003;58:263–7. 13. Klöcking R, Helbig B. Medical aspects and applications of humic substances. In: Steibüchel A, Marshessault RH, editors. Biopolymers of medical and pharmaceutical applications. Weinheim: Wiley-VCH Verlag, 2005. p. 3–16. 14. Schepetkin I, Khlebnikov A, Kwon BS. Medical drugs from humus matter: focus on mumie. Drug Dev Res 2002;57:140–59. 15. Schenkel JS, Jacobsen C, Rostetter C, Grätz KW, Rücker M, Gander T. Inferior alveolar nerve function after open reduction and internal fixation of mandibular fractures. J Craniomaxillofac Surg 2016;44:743–8. 16. Hackenberg B, Lee C, Caterson EJ. Management of subcondylar mandible fractures in the adult patient. J Craniofac Surg. 2014;25:166–71. 17. Altuntafl EE, Oztemur Z, Ozer H, Müderris S. Effect of extracorporeal shock waves on subcondylar mandibular fractures. J Craniofac Surg 2012;23:1645–8. 18. Huo MH, Troiano NW, Pelker RR, Gundberg CM, Friedlaender GE. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. J Orthop Res 1991;9:383–90. 19. Tkachenko SS, Rutski VV, Grachev IR. Reparative regeneration of the bone tissue under the effect of mumie-asyl. [Article in Russian] Ortop Travmatol Protez 1979;(11):49–52. 20. Kel’ginbaev NS, Sorokina VA, Stefanidu AG, Ismailova VN. Treatment of long tubular bone fractures with Mumie Assil preparations in experiments and clinical conditions. [Article in Russian] Eksp Khir Anesteziol 1973;18:31–5. 21. van Rensburg CE, Naude PJ. Potassium humate inhibits complement activation and the production of inflammatory cytokines in vitro. Inflammation 2009;32:270–6. 22. van Rensburg CE. The antiinflammatory properties of humic substances: a mini review. Phytother Res 2015;29:791–5.
Yıl 2017, Cilt: 7 Sayı: 2, 57 - 61, 30.09.2017

Öz

Kaynakça

  • 1. Schindeler A, McDonald MM, Bokko P, Little DG. Bone remodeling during fracture repair: the cellular picture. Semin Cell Dev Biol 2008;19:459–66. 2. Dimitriou R, Jones E, McGonagle D, Giannoudis PV. Bone regeneration: current concepts and future directions. BMC Med 2011:31;9:66. 3. Fischer C, Doll J, Tanner M, et al. Quantification of TGF-ß1, PDGF and IGF-1 cytokineexpression after fracture treatment vs. non-union therapy via masquelet. Injury 2016;47:342–9. 4. Tsiridis E, Upadhyay N, Giannoudis P. Molecular aspects of fracture healing: which are the important molecules? Injury 2007;38(Suppl 1):S11–25. 5. Moghaddam A, Müller U, Roth HJ, Wentzensen A, Grützner PA, Zimmermann G. TRACP 5b and CTX as osteological markers of delayed fracture healing. Injury 2011; 42:758–64. 6. Smith DD, Gowen M, Mundy GR. Effects of interferon-gamma and other cytokines on collagen synthesis in fetal rat bone cultures. Endocrinology 1987;120:2494–9. 7. Yang S, Ding W, Feng D, Gong H, Zhu D, Chen B, Chen J. Loss of B cell regulatory function is associated with delayed healing in patients with tibia fracture. APMIS 2015;123:975–85. 8. Asagiri M, Takayanagi H. The molecular understanding of osteoclast differentiation. Bone 2007;40:251–64. 9. Islam KMS, Schuhmacher A, Gropp JM. Humic acid substances in animal agriculture. Pakistan Journal of Nutrition 2005;4:126– 34. 10. Hu CW, Yen CC, Huang YL, Pan CH, Lu FJ, Chao MR. Oxidatively damaged DNA induced by humic acid and arsenic in maternal and neonatal mice. Chemosphere 2010;79:93–9. 11. Çal›fl›r M, Akp›nar A, Poyraz Ö, Göze F, Ç›nar Z. The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature-induced periodontitis in rats. J Periodontal Res 2016;51:499–507. 12. Jooné GK, Dekker J, van Rensburg CE. Investigation of the immunostimulatory properties of oxihumate. Z Naturforsch C 2003;58:263–7. 13. Klöcking R, Helbig B. Medical aspects and applications of humic substances. In: Steibüchel A, Marshessault RH, editors. Biopolymers of medical and pharmaceutical applications. Weinheim: Wiley-VCH Verlag, 2005. p. 3–16. 14. Schepetkin I, Khlebnikov A, Kwon BS. Medical drugs from humus matter: focus on mumie. Drug Dev Res 2002;57:140–59. 15. Schenkel JS, Jacobsen C, Rostetter C, Grätz KW, Rücker M, Gander T. Inferior alveolar nerve function after open reduction and internal fixation of mandibular fractures. J Craniomaxillofac Surg 2016;44:743–8. 16. Hackenberg B, Lee C, Caterson EJ. Management of subcondylar mandible fractures in the adult patient. J Craniofac Surg. 2014;25:166–71. 17. Altuntafl EE, Oztemur Z, Ozer H, Müderris S. Effect of extracorporeal shock waves on subcondylar mandibular fractures. J Craniofac Surg 2012;23:1645–8. 18. Huo MH, Troiano NW, Pelker RR, Gundberg CM, Friedlaender GE. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. J Orthop Res 1991;9:383–90. 19. Tkachenko SS, Rutski VV, Grachev IR. Reparative regeneration of the bone tissue under the effect of mumie-asyl. [Article in Russian] Ortop Travmatol Protez 1979;(11):49–52. 20. Kel’ginbaev NS, Sorokina VA, Stefanidu AG, Ismailova VN. Treatment of long tubular bone fractures with Mumie Assil preparations in experiments and clinical conditions. [Article in Russian] Eksp Khir Anesteziol 1973;18:31–5. 21. van Rensburg CE, Naude PJ. Potassium humate inhibits complement activation and the production of inflammatory cytokines in vitro. Inflammation 2009;32:270–6. 22. van Rensburg CE. The antiinflammatory properties of humic substances: a mini review. Phytother Res 2015;29:791–5.
Toplam 1 adet kaynakça vardır.

Ayrıntılar

Konular Sağlık Kurumları Yönetimi
Bölüm Makaleler
Yazarlar

Kasım Durmuş

Adem Bora Bu kişi benim

Mehtap Doğan Bu kişi benim

Hatice Özer Bu kişi benim

Ersin Tuncer Bu kişi benim

Emine Elif Altuntaş Bu kişi benim

Yayımlanma Tarihi 30 Eylül 2017
Gönderilme Tarihi 31 Ekim 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 7 Sayı: 2

Kaynak Göster

APA Durmuş, K., Bora, A., Doğan, M., Özer, H., vd. (2017). Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates, 7(2), 57-61.
AMA Durmuş K, Bora A, Doğan M, Özer H, Tuncer E, Altuntaş EE. Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates. Eylül 2017;7(2):57-61.
Chicago Durmuş, Kasım, Adem Bora, Mehtap Doğan, Hatice Özer, Ersin Tuncer, ve Emine Elif Altuntaş. “Can Local Administration of Humic Acid Shorten Recovery Time of Mandibular Fractures? Experimental Study”. ENT Updates 7, sy. 2 (Eylül 2017): 57-61.
EndNote Durmuş K, Bora A, Doğan M, Özer H, Tuncer E, Altuntaş EE (01 Eylül 2017) Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates 7 2 57–61.
IEEE K. Durmuş, A. Bora, M. Doğan, H. Özer, E. Tuncer, ve E. E. Altuntaş, “Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study”, ENT Updates, c. 7, sy. 2, ss. 57–61, 2017.
ISNAD Durmuş, Kasım vd. “Can Local Administration of Humic Acid Shorten Recovery Time of Mandibular Fractures? Experimental Study”. ENT Updates 7/2 (Eylül 2017), 57-61.
JAMA Durmuş K, Bora A, Doğan M, Özer H, Tuncer E, Altuntaş EE. Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates. 2017;7:57–61.
MLA Durmuş, Kasım vd. “Can Local Administration of Humic Acid Shorten Recovery Time of Mandibular Fractures? Experimental Study”. ENT Updates, c. 7, sy. 2, 2017, ss. 57-61.
Vancouver Durmuş K, Bora A, Doğan M, Özer H, Tuncer E, Altuntaş EE. Can local administration of humic acid shorten recovery time of mandibular fractures? Experimental study. ENT Updates. 2017;7(2):57-61.