Year 2019, Volume 5, Issue 1, Pages 148 - 158 2019-01-04

Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods

Zeliha Türkyılmaz [1] , Ahmet Hatipoğlu [2] , Mahmut Yüksel [3] , Nurettin Aydoğdu [4] , Gülara Hüseyinova [5]

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Objectives: Liver transplantation is increasingly being used in the treatment of end-stage liver disease. Ischemia-reperfusion injury is one of the major problems encountered in transplantation. In this study, we aimed to compare the effects of melatonin, pentoxifylline, and dimethyl sulfoxide (DMSO), in hepatic ischemia-reperfusion injury with different methods such as biochemical/ultrastructural changes and hepatobiliary scintigraphy.

Methods: Thirty rabbits were used in the Laboratory of Experimental Animals of Trakya University under appropriate conditions. Sham laparotomy and only ischemia reperfusion group were planned. They were used melatonin, pentoxifylline, and DMSO after ischemia-reperfusion in the other three groups. 6 rabbits were randomly selected for each group. Rabbits in all groups were subjected to liver scintigraphy. Following scintigraphy, 2 cm2 of liver tissue was removed to examining for liver antioxidant enzyme levels (superoxide dismutase [SOD] and glutathione peroxidase [GPx]) and for liver electron microscopy.

Results: Pentoxifylline and melatonin protected significantly uptake and excretion functions in liver scintigraphy. When the effects of all three substances were examined by electron microscopy, it was found that the three substances protected the liver from the effects of ischemia-reperfusion damage at varying rates. All three agents were found to protect SOD and GPx from falling in various amounts.

Conclusions: Studies to prevent ischemia-reperfusion injury, which may develop as a result of the Pringle maneuver applied to liver transplantations as well as to liver resections or liver injuries, still maintain their popularity. In our study, the effects of agents were identified in three different ways. Ischemia-reperfusion injury-reducing effect of pentoxifylline gave parallel results with three methods. 

Pentoxifylline, melatonin, dimethyl sulfoxide, ischemia, reperfusion
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Primary Language en
Subjects Health Care Sciences and Services
Journal Section Original Articles

Orcid: 0000-0002-0012-2089
Author: Zeliha Türkyılmaz (Primary Author)
Institution: Department of General Surgery, Edirne 1. Murat State Hospital, Edirne, Turkey
Country: Turkey

Orcid: 0000-0003-2691-2529
Author: Ahmet Hatipoğlu
Institution: Department of General Surgery, Trakya University School of Medicine, Edirne, Turkey
Country: Turkey

Orcid: 0000-0003-0159-6873
Author: Mahmut Yüksel
Institution: Department of Nucleer Medicine, Altınbaş University Medical Park Bahçelievler Hostipal, İstanbul, Turkey
Country: Turkey

Orcid: 0000-0002-6341-5137
Author: Nurettin Aydoğdu
Institution: Department of Physiology, Trakya University School of Medicine, Edirne, Turkey
Country: Turkey

Orcid: 0000-0001-7941-2080
Author: Gülara Hüseyinova
Institution: Department of Pathology, Trakya University School of Medicine, Edirne, Turkey
Country: Turkey


Publication Date: January 4, 2019

EndNote %0 The European Research Journal Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods %A Zeliha Türkyılmaz , Ahmet Hatipoğlu , Mahmut Yüksel , Nurettin Aydoğdu , Gülara Hüseyinova %T Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods %D 2019 %J The European Research Journal %P -2149-3189 %V 5 %N 1 %R doi: 10.18621/eurj.421296 %U 10.18621/eurj.421296